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Molecular characterization of an estrogen receptor and estrogen-related receptor and their autoregulatory capabilities in two Mytilus species

Nagasawa, Kazue; Treen, Nicholas; Kondo, Reki; Otoki, Yurika; Itoh, Naoki; Rotchell, Jeanette M.; Osada, Makoto

Authors

Kazue Nagasawa

Nicholas Treen

Reki Kondo

Yurika Otoki

Naoki Itoh

Makoto Osada



Abstract

Vertebrate-like sex steroid hormones have been widely detected in mollusks, and numerous experiments have shown the importance of steroids in gonad development. Nevertheless, their signaling pathways in invertebrates have not been uncovered yet. Steroid receptors are an ancient class of transcription factors with multiple roles in not only vertebrates but also invertebrates. Estrogen signaling is thought to have major roles in mollusk physiology, but the full repertoire of estrogen receptors is unknown. We presented the successful cloning of two novel forms of estrogen receptor-like genes. These receptors are present in two closely related species of Mytilus: Mytilus edulis and Mytilus galloprovincialis, commonly known and widely distributed sentinel species. Our phylogenetic analysis revealed that one of these receptors is an estrogen receptor (ER) and the other one is an estrogen-related receptor (ERR). Studies of expression analysis showed that both receptor mRNAs were localized in the oocytes and follicle cells in contact with developing oocytes in the ovary and Sertoli cells in the testis, and in the ciliated cells of the gill. In addition, we have evidence that one (ER) of these may have a capacity to autoregulate its own expression in the gonadal cells by estrogen (E2) and that this gene is responsive to estrogenic compounds.

Journal Article Type Article
Publication Date Jun 15, 2015
Journal Gene
Print ISSN 0378-1119
Electronic ISSN 1879-0038
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 564
Issue 2
Pages 153-159
Institution Citation Nagasawa, K., Treen, N., Kondo, R., Otoki, Y., Itoh, N., Rotchell, J. M., & Osada, M. (2015). Molecular characterization of an estrogen receptor and estrogen-related receptor and their autoregulatory capabilities in two Mytilus species. Gene, 564(2), 153-159. https://doi.org/10.1016/j.gene.2015.03.073
DOI https://doi.org/10.1016/j.gene.2015.03.073
Keywords Mussels; Reproduction; Endocrine disruption; Autoregulation
Publisher URL http://www.sciencedirect.com/science/article/pii/S0378111915003996
Copyright Statement © 2016, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/
Additional Information Author's accepted manuscript of article published in: Gene, 2015, v.564, issue 2.

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Copyright Statement
© 2016, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/









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