Structural insights into magnetic clusters grown inside virus capsids

Jaafar, M.; Aljabali, A. A. A.; Berlanga, I.; Mas-Ballesté, R.; Saxena, P.; Warren, S.; Lomonossoff, G. P.; Evans, D. J.; de Pablo, P. J.

Abstract

Magnetic nanoparticles have multiple applications in materials science. In particular, virus capsids have been suggested as promising templates for building up nanometric-sized magnetic clusters by taking advantage of their inner cavity as a nanoreactor. In this study we investigate the magnetization of individual cobalt-filled cowpea mosaic virus empty virus-like particles using atomic force microscopy. We also combine the analysis of the effects of dehydration on the structure of virus particles with a comparison of their magnetic signal to that provided by commercially available magnetic nanoparticles of similar size. These two approaches allow the evaluation of the structure of the metallic cluster grown inside the virus capsid. We conclude that, rather than forming solid clusters, cobalt inside viruses forms a discontinuous structure that does not completely fill the virus cavity and reaches about 10% of its volume.

Citation

Jaafar, M., Aljabali, A. A. A., Berlanga, I., Mas-Ballesté, R., Saxena, P., Warren, S., Lomonossoff, G. P., Evans, D. J., & de Pablo, P. J. (2014). Structural insights into magnetic clusters grown inside virus capsids. ACS Applied Materials & Interfaces, 6(23), 20936-20942. https://doi.org/10.1021/am505682x

• Acceptance Date: Nov 18, 2014
• Online Publication Date: Nov 26, 2014
• Publication Date: Dec 10, 2014
• Deposit Date: Apr 17, 2015
• Publicly Available Date: Apr 17, 2015
• Journal: ACS applied materials & interfaces
• Print ISSN: 1944-8244
• Publisher: American Chemical Society
• Peer Reviewed: Peer Reviewed
• Volume: 6
• Issue: 23
• Pages: 20936-20942
• DOI: https://doi.org/10.1021/am505682x
• Keywords: Magnetic nanoparticles; Nanoreactors; Protein cages; Virus capsid; Magnetic force microscopy
• Public URL: https://hull-repository.worktribe.com/output/372636
• Publisher URL: http://pubs.acs.org/doi/abs/10.1021/am505682x
• Additional Information: This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS applied materials & interfaces, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://dx.doi.org/10.1021/am505682x
• Contract Date: Apr 17, 2015