Prevalence and properties of mecC methicillin-resistant Staphylococcus aureus (MRSA) in bovine bulk tank milk in Great Britain

Paterson, G. K.; Morgan, F. J. E.; Harrison, E. M.; Peacock, S. J.; Parkhill, J.; Zadoks, R. N.; Holmes, M. A.

Abstract

Objectives mecC methicillin-resistant Staphylococcus aureus (MRSA) represent a newly recognized form of MRSA, distinguished by the possession of a divergent mecA homologue, mecC. The first isolate to be identified came from bovine milk, but there are few data on the prevalence of mecC MRSA among dairy cattle. The aim of this study was to conduct a prevalence study of mecC MRSA among dairy farms in Great Britain. Methods Test farms were randomly selected by random order generation and bulk tank samples were tested for the presence of mecC MRSA by broth enrichment and plating onto chromogenic agar. All MRSA isolated were screened by PCR for mecA and mecC, and mecC MRSA were further characterized by multilocus sequence typing, spa typing and antimicrobial susceptibility testing. Results mecC MRSA were detected on 10 of 465 dairy farms sampled in England and Wales (prevalence 2.15%, 95% CI 1.17%–3.91%), but not from 625 farms sampled in Scotland (95% CI of prevalence 0%–0.61%). Seven isolates belonged to sequence type (ST) 425, while the other three belonged to clonal complex 130. Resistance to non-β-lactam antibiotics was uncommon. All 10 isolates produced a negative result by slide agglutination for penicillin-binding protein 2a. mecA MRSA ST398 was detected on one farm in England. Conclusions mecC MRSA is widely distributed among dairy farms in Great Britain, but this distribution is not uniform across the whole country. These results provide an important baseline dataset to monitor the epidemiology of this emerging form of MRSA.

Citation

Paterson, G. K., Morgan, F. J. E., Harrison, E. M., Peacock, S. J., Parkhill, J., Zadoks, R. N., & Holmes, M. A. (2014). Prevalence and properties of mecC methicillin-resistant Staphylococcus aureus (MRSA) in bovine bulk tank milk in Great Britain. The journal of antimicrobial chemotherapy, 69(3), 598-602. https://doi.org/10.1093/jac/dkt417

• Journal Article Type: Article
• Acceptance Date: Sep 25, 2013
• Online Publication Date: Oct 23, 2013
• Publication Date: Mar 1, 2014
• Deposit Date: Jun 12, 2015
• Publicly Available Date: Jun 12, 2015
• Journal: Journal of antimicrobial chemotherapy
• Print ISSN: 0305-7453
• Publisher: Oxford University Press
• Peer Reviewed: Peer Reviewed
• Volume: 69
• Issue: 3
• Pages: 598-602
• DOI: https://doi.org/10.1093/jac/dkt417
• Keywords: Bovine mastitis; Antibiotic resistance; Molecular epidemiology
• Public URL: https://hull-repository.worktribe.com/output/375187
• Publisher URL: https://academic.oup.com/jac/article/69/3/598/785208
• Additional Information: Copy of article originally published: Journal of antimicrobial chemotherapy, 2014, v.69, issue 3
• Contract Date: Jun 12, 2015