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Isolation and characterization of polymorphic microsatellite loci in the endangered Portuguese freshwater fish Squalius aradensis (Cyprinidae)

Ze-Ze, L.; Hanfling, B; Mesquita, N.; Haenfling, Bernd; Cunha, C.; Hänfling, B.; Carvalho, G. R.; Zé-Zé, L.; Tenreiro, R.; Coelho, M. M.

Authors

L. Ze-Ze

B Hanfling

N. Mesquita

Bernd Haenfling

C. Cunha

B. Hänfling

G. R. Carvalho

L. Zé-Zé

R. Tenreiro

M. M. Coelho



Abstract

Here we describe the isolation and characterization of six polymorphic microsatellite loci for Squalius aradensis. The number of observed alleles per locus ranged from three to 16 and the observed heterozygosity from 0.22 to 0.95. These primers will be useful in determining the population structure of S. aradensis and for conservation genetics of this endangered and endemic species. Furthermore, successful cross-species amplification in S. alburnoides and Chondrostoma lusitanicum suggests that a wider amplification of these markers is possible.

Citation

Mesquita, N., Cunha, C., Hänfling, B., Carvalho, G. R., Zé-Zé, L., Tenreiro, R., & Coelho, M. M. (2003). Isolation and characterization of polymorphic microsatellite loci in the endangered Portuguese freshwater fish Squalius aradensis (Cyprinidae). Molecular ecology notes, 3(4), 572-574. https://doi.org/10.1046/j.1471-8286.2003.00515.x

Journal Article Type Article
Acceptance Date Dec 31, 2003
Publication Date Dec 1, 2003
Publicly Available Date Mar 29, 2024
Journal MOLECULAR ECOLOGY NOTES
Print ISSN 1471-8278
Electronic ISSN 1471-8286
Peer Reviewed Peer Reviewed
Volume 3
Issue 4
Pages 572-574
DOI https://doi.org/10.1046/j.1471-8286.2003.00515.x
Keywords General Biochemistry, Genetics and Molecular Biology; Ecology; Biochemistry
Public URL https://hull-repository.worktribe.com/output/391277