Isolation and characterization of polymorphic microsatellite loci in the endangered Portuguese freshwater fish Squalius aradensis (Cyprinidae)

Ze-Ze, L.; Hanfling, B; Mesquita, N.; Haenfling, Bernd; Cunha, C.; Hänfling, B.; Carvalho, G. R.; Zé-Zé, L.; Tenreiro, R.; Coelho, M. M.

Abstract

Here we describe the isolation and characterization of six polymorphic microsatellite loci for Squalius aradensis. The number of observed alleles per locus ranged from three to 16 and the observed heterozygosity from 0.22 to 0.95. These primers will be useful in determining the population structure of S. aradensis and for conservation genetics of this endangered and endemic species. Furthermore, successful cross-species amplification in S. alburnoides and Chondrostoma lusitanicum suggests that a wider amplification of these markers is possible.

Citation

Mesquita, N., Cunha, C., Hänfling, B., Carvalho, G. R., Zé-Zé, L., Tenreiro, R., & Coelho, M. M. (2003). Isolation and characterization of polymorphic microsatellite loci in the endangered Portuguese freshwater fish Squalius aradensis (Cyprinidae). Molecular ecology notes, 3(4), 572-574. https://doi.org/10.1046/j.1471-8286.2003.00515.x

• Journal Article Type: Article
• Acceptance Date: Dec 31, 2003
• Publication Date: Dec 1, 2003
• Journal: MOLECULAR ECOLOGY NOTES
• Print ISSN: 1471-8278
• Peer Reviewed: Peer Reviewed
• Volume: 3
• Issue: 4
• Pages: 572-574
• DOI: https://doi.org/10.1046/j.1471-8286.2003.00515.x
• Keywords: General Biochemistry, Genetics and Molecular Biology; Ecology; Biochemistry
• Public URL: https://hull-repository.worktribe.com/output/391277