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Isolation and characterization of polymorphic microsatellite loci in the endangered Portuguese freshwater fish Squalius aradensis (Cyprinidae)

Ze-Ze, L.; Hanfling, B; Mesquita, N.; Haenfling, Bernd; Cunha, C.; Hänfling, B.; Carvalho, G. R.; Zé-Zé, L.; Tenreiro, R.; Coelho, M. M.

Authors

L. Ze-Ze

Dr Bernd Haenfling B.Haenfling@hull.ac.uk
Senior Lecturer/ Chair of the Evolutionary Biology Group (EvoHull)

N. Mesquita

Dr Bernd Haenfling B.Haenfling@hull.ac.uk
Senior Lecturer/ Chair of the Evolutionary Biology Group (EvoHull)

C. Cunha

Dr Bernd Haenfling B.Haenfling@hull.ac.uk
Senior Lecturer/ Chair of the Evolutionary Biology Group (EvoHull)

G. R. Carvalho

L. Zé-Zé

R. Tenreiro

M. M. Coelho



Abstract

Here we describe the isolation and characterization of six polymorphic microsatellite loci for Squalius aradensis. The number of observed alleles per locus ranged from three to 16 and the observed heterozygosity from 0.22 to 0.95. These primers will be useful in determining the population structure of S. aradensis and for conservation genetics of this endangered and endemic species. Furthermore, successful cross-species amplification in S. alburnoides and Chondrostoma lusitanicum suggests that a wider amplification of these markers is possible.

Journal Article Type Article
Publication Date Dec 1, 2003
Journal MOLECULAR ECOLOGY NOTES
Print ISSN 1471-8278
Electronic ISSN 1471-8286
Peer Reviewed Peer Reviewed
Volume 3
Issue 4
Pages 572-574
APA6 Citation Mesquita, N., Cunha, C., Hänfling, B., Carvalho, G. R., Zé-Zé, L., Tenreiro, R., & Coelho, M. M. (2003). Isolation and characterization of polymorphic microsatellite loci in the endangered Portuguese freshwater fish Squalius aradensis (Cyprinidae). Molecular ecology notes, 3(4), 572-574. https://doi.org/10.1046/j.1471-8286.2003.00515.x
DOI https://doi.org/10.1046/j.1471-8286.2003.00515.x
Keywords General Biochemistry, Genetics and Molecular Biology; Ecology; Biochemistry