Rapid and direct magnetization of GFP-reporter yeast for micro-screening systems
García-Alonso, Javier; Fakhrullin, Rawil F.; Paunov, Vesselin N.
Rawil F. Fakhrullin
Vesselin N. Paunov V.N.Paunov@hull.ac.uk
Saccharomyces cerevisiae containing fluorescent markers are ideal candidates for applications in microfluidic screening systems as fluorescence signal is emitted without the need of additional reagents. Here we develop a method for magnetic functionalisation of such cells which allows their handling and immobilization in micro-screening devices. After exposure of the magnetized GFP-reporter yeast (GreenScreen™) to a genotoxic compound, the fluorescence emission was detected using fluorescent spectrometer and an Epi-fluorescent microscope. Results demonstrate that GFP production and fluorescence emission is not altered by their magnetic functionalization, indicating its potential employment on biosensors, bioreactors and micro-screening studies. © 2009 Elsevier B.V.
García-Alonso, J., Fakhrullin, R. F., & Paunov, V. N. (2010). Rapid and direct magnetization of GFP-reporter yeast for micro-screening systems. Biosensors & bioelectronics, 25(7), 1816-1819. https://doi.org/10.1016/j.bios.2009.11.016
|Journal Article Type||Article|
|Acceptance Date||Nov 17, 2009|
|Online Publication Date||Nov 24, 2009|
|Publication Date||Mar 15, 2010|
|Journal||BIOSENSORS & BIOELECTRONICS|
|Peer Reviewed||Peer Reviewed|
|Keywords||Cell magnetization; GFP; Toxicity screening; Magnetic nanoparticles|
This file is under embargo due to copyright reasons.
You might also like
Advanced biomedical applications based on emerging 3D cell culturing platforms