Rapid and direct magnetization of GFP-reporter yeast for micro-screening systems

García-Alonso, Javier; Fakhrullin, Rawil F.; Paunov, Vesselin N.

Abstract

Saccharomyces cerevisiae containing fluorescent markers are ideal candidates for applications in microfluidic screening systems as fluorescence signal is emitted without the need of additional reagents. Here we develop a method for magnetic functionalisation of such cells which allows their handling and immobilization in micro-screening devices. After exposure of the magnetized GFP-reporter yeast (GreenScreen™) to a genotoxic compound, the fluorescence emission was detected using fluorescent spectrometer and an Epi-fluorescent microscope. Results demonstrate that GFP production and fluorescence emission is not altered by their magnetic functionalization, indicating its potential employment on biosensors, bioreactors and micro-screening studies. © 2009 Elsevier B.V.

Citation

García-Alonso, J., Fakhrullin, R. F., & Paunov, V. N. (2010). Rapid and direct magnetization of GFP-reporter yeast for micro-screening systems. Biosensors & bioelectronics, 25(7), 1816-1819. https://doi.org/10.1016/j.bios.2009.11.016

• Journal Article Type: Article
• Acceptance Date: Nov 17, 2009
• Online Publication Date: Nov 24, 2009
• Publication Date: Mar 15, 2010
• Journal: BIOSENSORS & BIOELECTRONICS
• Print ISSN: 0956-5663
• Electronic ISSN: 1873-4235
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 25
• Issue: 7
• Pages: 1816-1819
• DOI: https://doi.org/10.1016/j.bios.2009.11.016
• Keywords: Cell magnetization; GFP; Toxicity screening; Magnetic nanoparticles
• Public URL: https://hull-repository.worktribe.com/output/391304
• Publisher URL: https://www.sciencedirect.com/science/article/pii/S0956566309006241?via%3Dihub