Rapid and direct magnetization of GFP-reporter yeast for micro-screening systems
García-Alonso, Javier; Fakhrullin, Rawil F.; Paunov, Vesselin N.
Rawil F. Fakhrullin
Professor Vesselin Paunov V.N.Paunov@hull.ac.uk
Professor of Physical Chemistry and Advanced Materials
Saccharomyces cerevisiae containing fluorescent markers are ideal candidates for applications in microfluidic screening systems as fluorescence signal is emitted without the need of additional reagents. Here we develop a method for magnetic functionalisation of such cells which allows their handling and immobilization in micro-screening devices. After exposure of the magnetized GFP-reporter yeast (GreenScreen™) to a genotoxic compound, the fluorescence emission was detected using fluorescent spectrometer and an Epi-fluorescent microscope. Results demonstrate that GFP production and fluorescence emission is not altered by their magnetic functionalization, indicating its potential employment on biosensors, bioreactors and micro-screening studies. © 2009 Elsevier B.V.
|Journal Article Type||Article|
|Publication Date||Mar 15, 2010|
|Journal||BIOSENSORS & BIOELECTRONICS|
|Peer Reviewed||Peer Reviewed|
|APA6 Citation||García-Alonso, J., Fakhrullin, R. F., & Paunov, V. N. (2010). Rapid and direct magnetization of GFP-reporter yeast for micro-screening systems. Biosensors & bioelectronics, 25(7), 1816-1819. https://doi.org/10.1016/j.bios.2009.11.016|
|Keywords||Cell magnetization; GFP; Toxicity screening; Magnetic nanoparticles|
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