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Rapid and direct magnetization of GFP-reporter yeast for micro-screening systems

García-Alonso, Javier; Fakhrullin, Rawil F.; Paunov, Vesselin N.

Authors

Javier García-Alonso

Rawil F. Fakhrullin

Vesselin N. Paunov V.N.Paunov@hull.ac.uk



Abstract

Saccharomyces cerevisiae containing fluorescent markers are ideal candidates for applications in microfluidic screening systems as fluorescence signal is emitted without the need of additional reagents. Here we develop a method for magnetic functionalisation of such cells which allows their handling and immobilization in micro-screening devices. After exposure of the magnetized GFP-reporter yeast (GreenScreen™) to a genotoxic compound, the fluorescence emission was detected using fluorescent spectrometer and an Epi-fluorescent microscope. Results demonstrate that GFP production and fluorescence emission is not altered by their magnetic functionalization, indicating its potential employment on biosensors, bioreactors and micro-screening studies. © 2009 Elsevier B.V.

Citation

GarcĂ­a-Alonso, J., Fakhrullin, R. F., & Paunov, V. N. (2010). Rapid and direct magnetization of GFP-reporter yeast for micro-screening systems. Biosensors & bioelectronics, 25(7), 1816-1819. https://doi.org/10.1016/j.bios.2009.11.016

Journal Article Type Article
Acceptance Date Nov 17, 2009
Online Publication Date Nov 24, 2009
Publication Date Mar 15, 2010
Journal BIOSENSORS & BIOELECTRONICS
Print ISSN 0956-5663
Electronic ISSN 1873-4235
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 25
Issue 7
Pages 1816-1819
DOI https://doi.org/10.1016/j.bios.2009.11.016
Keywords Cell magnetization; GFP; Toxicity screening; Magnetic nanoparticles
Public URL https://hull-repository.worktribe.com/output/391304
Publisher URL https://www.sciencedirect.com/science/article/pii/S0956566309006241?via%3Dihub