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Anchoring novel molecular biomarker responses to traditional responses in fish exposed to environmental contamination

Pacheco, Mário; Nogueira, Patrícia; Lourdes Pereira, M.; Mendo, Sónia; Rotchell, Jeanette M.

Authors

Mário Pacheco

Patrícia Nogueira

M. Lourdes Pereira

Sónia Mendo



Abstract

The responses of Dicentrarchus labrax and Liza aurata to aquatic pollution were assessed in a contaminated coastal lagoon, using both traditional and novel biomarkers combined. DNA damage, assessed by comet assay, was higher in both fish species from the contaminated sites, whereas levels of cytochrome P450 1A1 gene expression were not significantly altered. The liver histopathological analysis also revealed significant lesions in fish from contaminated sites. Alterations in ras and xpf genes were analysed and additional pollutant-responsive genes were identified. While no alterations were found in ras gene, a downregulation of xpf gene was observed in D. labrax from a contaminated site. Suppression subtractive hybridization applied to D. labrax collected at a contaminated site, revealed altered expression in genes involved in energy metabolism, immune system activity and antioxidant response. The approach and results reported herein demonstrate the utility of anchoring traditional biomarker responses alongside novel biomarker responses. (C) 2009 Elsevier Ltd. All rights reserved.

Citation

Nogueira, P., Pacheco, M., Lourdes Pereira, M., Mendo, S., & Rotchell, J. M. (2010). Anchoring novel molecular biomarker responses to traditional responses in fish exposed to environmental contamination. Environmental pollution, 158(5), (1783-1790). doi:10.1016/j.envpol.2009.11.007. ISSN 0269-7491

Journal Article Type Article
Acceptance Date Nov 6, 2009
Online Publication Date Nov 26, 2009
Publication Date 2010-05
Journal ENVIRONMENTAL POLLUTION
Print ISSN 0269-7491
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 158
Issue 5
Pages 1783-1790
DOI https://doi.org/10.1016/j.envpol.2009.11.007
Keywords Pollution; Biomarker; Toxicology
Public URL https://hull-repository.worktribe.com/output/400013