Ras gene in marine mussels: A molecular level response to petrochemical exposure

Rotchell, J. M.; Rendon-Von Osten, J.; Soares, A. Mn. M.; Lima, I.; Peck, M. R.; Rendón-Von Osten, J.; Soares, A. M. V. M.; Guilhermino, L.; Rotchell, Jeanette

Abstract

Mussels are susceptible to numerous toxicants and are often employed as bioindicators. This study investigated the status of the ras proto-oncogene in Mytilus galloprovincialis following petrochemical exposure. A M. galloprovincialis homologue of the vertebrate ras gene was isolated, showing conserved sequence in regions of functional importance and a high incidence of polymorphic variation. Mutational damage was investigated in mussels chronically exposed to the water-accommodated fraction of #4 fuel-oil (WAF), and in mussels collected along the NW coast of Portugal in sites with different levels of petrochemical contamination. A ras gene point mutation was identified in the codon 35 of one individual exposed to 12.5% WAF. No mutations were detected in mussels from the WAF control or environmental samples. This represents the first report of a ras gene mutation, experimentally-induced by petrochemical exposure, in an invertebrate species. © 2008 Elsevier Ltd. All rights reserved.

Citation

Lima, I., Peck, M. R., Rendón-Von Osten, J., Soares, A. M. V. M., Guilhermino, L., & Rotchell, J. (2008). Ras gene in marine mussels: A molecular level response to petrochemical exposure. Marine pollution bulletin, 56(4), 633-640. https://doi.org/10.1016/j.marpolbul.2008.01.018

• Journal Article Type: Article
• Online Publication Date: Mar 7, 2008
• Publication Date: 2008-04
• Journal: MARINE POLLUTION BULLETIN
• Print ISSN: 0025-326X
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 56
• Issue: 4
• Pages: 633-640
• DOI: https://doi.org/10.1016/j.marpolbul.2008.01.018
• Keywords: ras gene Mytilus galloprovincialis mutation biomarker genotoxicity petroleum hydrocarbon polycyclic aromatic-hydrocarbons mytilus-edulis complex dna-adducts mya-arenaria oncogene mutations hemic neoplasia softshell clams macoma-balthica harbor sediment bl
• Public URL: https://hull-repository.worktribe.com/output/400021
• Publisher URL: https://www.sciencedirect.com/science/article/pii/S0025326X0800026X?via%3Dihub