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Ras gene in marine mussels: A molecular level response to petrochemical exposure

Rotchell, J. M.; Rendon-Von Osten, J.; Soares, A. Mn. M.; Lima, I.; Peck, M. R.; Rendón-Von Osten, J.; Soares, A. M. V. M.; Guilhermino, L.; Rotchell, Jeanette

Authors

J. Rendon-Von Osten

A. Mn. M. Soares

I. Lima

M. R. Peck

J. Rendón-Von Osten

A. M. V. M. Soares

L. Guilhermino



Abstract

Mussels are susceptible to numerous toxicants and are often employed as bioindicators. This study investigated the status of the ras proto-oncogene in Mytilus galloprovincialis following petrochemical exposure. A M. galloprovincialis homologue of the vertebrate ras gene was isolated, showing conserved sequence in regions of functional importance and a high incidence of polymorphic variation. Mutational damage was investigated in mussels chronically exposed to the water-accommodated fraction of #4 fuel-oil (WAF), and in mussels collected along the NW coast of Portugal in sites with different levels of petrochemical contamination. A ras gene point mutation was identified in the codon 35 of one individual exposed to 12.5% WAF. No mutations were detected in mussels from the WAF control or environmental samples. This represents the first report of a ras gene mutation, experimentally-induced by petrochemical exposure, in an invertebrate species. © 2008 Elsevier Ltd. All rights reserved.

Journal Article Type Article
Publication Date 2008-04
Journal MARINE POLLUTION BULLETIN
Print ISSN 0025-326X
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 56
Issue 4
Pages 633-640
APA6 Citation Lima, I., Peck, M. R., Rendón-Von Osten, J., Soares, A. M. V. M., Guilhermino, L., & Rotchell, J. (2008). Ras gene in marine mussels: A molecular level response to petrochemical exposure. Marine pollution bulletin, 56(4), (633-640). doi:10.1016/j.marpolbul.2008.01.018. ISSN 0025-326X
DOI https://doi.org/10.1016/j.marpolbul.2008.01.018
Keywords ras gene Mytilus galloprovincialis mutation biomarker genotoxicity petroleum hydrocarbon polycyclic aromatic-hydrocarbons mytilus-edulis complex dna-adducts mya-arenaria oncogene mutations hemic neoplasia softshell clams macoma-balthica harbor sediment bl
Publisher URL https://www.sciencedirect.com/science/article/pii/S0025326X0800026X?via%3Dihub
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