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Ras gene in marine mussels: A molecular level response to petrochemical exposure

Rotchell, J. M.; Rendon-Von Osten, J.; Soares, A. Mn. M.; Lima, I.; Peck, M. R.; Rendón-Von Osten, J.; Soares, A. M. V. M.; Guilhermino, L.; Rotchell, Jeanette


J. Rendon-Von Osten

A. Mn. M. Soares

I. Lima

M. R. Peck

J. Rendón-Von Osten

A. M. V. M. Soares

L. Guilhermino


Mussels are susceptible to numerous toxicants and are often employed as bioindicators. This study investigated the status of the ras proto-oncogene in Mytilus galloprovincialis following petrochemical exposure. A M. galloprovincialis homologue of the vertebrate ras gene was isolated, showing conserved sequence in regions of functional importance and a high incidence of polymorphic variation. Mutational damage was investigated in mussels chronically exposed to the water-accommodated fraction of #4 fuel-oil (WAF), and in mussels collected along the NW coast of Portugal in sites with different levels of petrochemical contamination. A ras gene point mutation was identified in the codon 35 of one individual exposed to 12.5% WAF. No mutations were detected in mussels from the WAF control or environmental samples. This represents the first report of a ras gene mutation, experimentally-induced by petrochemical exposure, in an invertebrate species. © 2008 Elsevier Ltd. All rights reserved.


Lima, I., Peck, M. R., Rendón-Von Osten, J., Soares, A. M. V. M., Guilhermino, L., & Rotchell, J. (2008). Ras gene in marine mussels: A molecular level response to petrochemical exposure. Marine pollution bulletin, 56(4), 633-640.

Journal Article Type Article
Online Publication Date Mar 7, 2008
Publication Date 2008-04
Print ISSN 0025-326X
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 56
Issue 4
Pages 633-640
Keywords ras gene Mytilus galloprovincialis mutation biomarker genotoxicity petroleum hydrocarbon polycyclic aromatic-hydrocarbons mytilus-edulis complex dna-adducts mya-arenaria oncogene mutations hemic neoplasia softshell clams macoma-balthica harbor sediment bl
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