Xiaohui Wang
Lipid Droplet-Specific Red Aggregation-Induced Emission Luminogens: Fast Light-Up of Gram-Positive Pathogens for Identification of Bacteria
Wang, Xiaohui; Song, Meirong; Liu, Yiwei; Feng, Xing; Redshaw, Carl; Wang, Dong; Zhu, Kui; Li, Ying; Tang, Ben Zhong
Authors
Meirong Song
Yiwei Liu
Xing Feng
Professor Carl Redshaw C.Redshaw@hull.ac.uk
Professor of Inorganic Materials Chemistry and REF Lead for Chemistry
Dong Wang
Kui Zhu
Ying Li
Ben Zhong Tang
Abstract
Gram-positive (G+) and Gram-negative (G-) bacteria require considerable differences in their therapeutic strategies. Thus, the development of highly efficient techniques for differentiating G+and G-bacteria is of vital importance for practical clinical applications. Herein, we present 2-((10-ethyl-10H-phenothiazin-3-yl)methylene)-1H-indene-1,3(2H)-dione(PH-ID), which is an electrically neutral, red fluorophore with aggregation-induced enhanced emission (AIEE) characteristic, to selectively stain G+bacteria membranes with high specificity and sensitivity. The staining result can be accomplished within 10 min and can be read by the naked eye. Lipopolysaccharide (LPS) in the outer leaflet of the membrane of G-bacteria prevents the staining of the bacteria by PH-ID, which is the mechanism underlying the Gram selectivity of PH-ID. This study not only provides a highly efficient method for bacteria imaging and discrimination, but also provides insight into the bacteria imaging mechanism, which is beneficial for the exploration of new electroneutral AIE luminogens for bacteria surveillance applications.
Citation
Wang, X., Song, M., Liu, Y., Feng, X., Redshaw, C., Wang, D., …Tang, B. Z. (2022). Lipid Droplet-Specific Red Aggregation-Induced Emission Luminogens: Fast Light-Up of Gram-Positive Pathogens for Identification of Bacteria. ACS Materials Letters, 4(8), 1523-1530. https://doi.org/10.1021/acsmaterialslett.2c00551
Journal Article Type | Article |
---|---|
Acceptance Date | Jul 1, 2022 |
Online Publication Date | Jul 18, 2022 |
Publication Date | Aug 1, 2022 |
Deposit Date | Jul 21, 2022 |
Publicly Available Date | Jul 19, 2023 |
Journal | ACS Materials Letters |
Print ISSN | 2639-4979 |
Electronic ISSN | 2639-4979 |
Publisher | American Chemical Society |
Peer Reviewed | Peer Reviewed |
Volume | 4 |
Issue | 8 |
Pages | 1523-1530 |
DOI | https://doi.org/10.1021/acsmaterialslett.2c00551 |
Keywords | General Materials Science; Biomedical Engineering; General Chemical Engineering |
Public URL | https://hull-repository.worktribe.com/output/4032967 |
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Copyright Statement
©2022 The authors.
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