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Metabolomic analysis of sex specific metabolites in gonads of the mussel, Mytilus edulis

Minier, Christophe; Cubero-Leon, Elena; Hill, Elizabeth M.; Rotchell, Jeanette

Authors

Christophe Minier

Elena Cubero-Leon

Elizabeth M. Hill



Abstract

Marine mussels have been used as sentinel organisms to monitor exposure to a variety of chemical contaminants, including endocrine disrupting chemicals, in the aquatic environment. Although they are an important species for use in ecotoxicology investigations, information on their reproductive physiology and biochemistry is fragmentary. Mass spectrometry-based profiling techniques are increasingly being used to study how the metabolome of an organism changes as a result of tissue differentiation, disease or in response to environmental stressors. In this study, ultraperformance liquid chromatography-time-of-flight- mass spectrometry (UPLC-TOFMS) was used to investigate sex specific differences in the mussel metabolome in order to further investigate the reproductive physiology of this species. Using this method, a comparison of female and male mantle tissues containing mature gonad, revealed significant differences in glycerophosphatidylcholine (PC) and lysophosphatidylcholine (LPC) metabolites. A number of other unidentified metabolites, including those putatively identified as conjugated sterols, were also differentially expressed between male and female mantle/gonadal tissue. © 2012 Elsevier Inc. All rights reserved.

Citation

Cubero-Leon, E., Minier, C., Hill, E. M., & Rotchell, J. (2012). Metabolomic analysis of sex specific metabolites in gonads of the mussel, Mytilus edulis. Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics, 7(2), (212-219). doi:10.1016/j.cbd.2012.03.002. ISSN 1744-117X

Journal Article Type Article
Acceptance Date Mar 12, 2012
Online Publication Date Mar 21, 2012
Publication Date Jun 1, 2012
Journal COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
Print ISSN 1744-117X
Electronic ISSN 1878-0407
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 7
Issue 2
Pages 212-219
DOI https://doi.org/10.1016/j.cbd.2012.03.002
Keywords Metabolomics; Mytilus edulis; Phospholipids; Sex; UPLC-TOFMS
Public URL https://hull-repository.worktribe.com/output/417615
Publisher URL https://www.sciencedirect.com/science/article/pii/S1744117X1200010X?via%3Dihub