Two CYP3A-like genes in the marine mussel Mytilus edulis: mRNA expression modulation following short-term exposure to endocrine disruptors

Rotchell, Jeanette; Cubero-Leon, Elena; Puinean, A. Mirel; Labadie, Pierre; Ciocan, Corina; Itoh, Naoki; Kishida, Mitsuyo; Osada, Makoto; Minier, Christophe; Hill, Elizabeth M.; Rotchell, Jeanette M.

Abstract

Members of the vertebrate CYP3A subfamily are involved in the metabolism of steroids and a wide range of xenobiotics. In this study two CYP3A- like mRNAs have been isolated from the mussel (Mytilus edulis), and their seasonal expression profile and modulation by estrogens examined. Sexual dimorphism of CYP3A- like mRNA expression was not observed in mussel gonads of individuals collected throughout a year. Nevertheless, natural variation in gonadal CYP3A- like mRNA expression was observed, with highest levels of CYP3A isoform1 and lowest levels of CYP3A isoform2 mRNA during the maturation and spawning season. Exposure to a 10% sewage treatment works extract did not result in any significant changes in mRNA expression of CYP3A-like. In contrast, exposure to E2 (200ng/L) and TBT (100ng/L) significantly down-regulated the expression of CYP3A- like isoform1 but not CYP3A-like isoform2 suggesting differential regulation. Understanding xenobiotic metabolism pathways in aquatic invertebrates.

• Journal Article Type: Article
• Publication Date: 2012-03
• Journal: Marine environmental research
• Print ISSN: 0141-1136
• Electronic ISSN: 1879-0291
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 74
• Pages: 32-39
• APA6 Citation: Cubero-Leon, E., Puinean, A. M., Labadie, P., Ciocan, C., Itoh, N., Kishida, M., …Rotchell, J. M. (2012). Two CYP3A-like genes in the marine mussel Mytilus edulis: mRNA expression modulation following short-term exposure to endocrine disruptors. Marine environmental research, 74, (32-39). doi:10.1016/j.marenvres.2011.11.012. ISSN 0141-1136
• DOI: https://doi.org/10.1016/j.marenvres.2011.11.012
• Keywords: Pollution; Ecotoxicology; Mussel; CYP3A
• Publisher URL: https://www.sciencedirect.com/science/article/pii/S0141113611001644?via%3Dihub