Two CYP3A-like genes in the marine mussel Mytilus edulis: mRNA expression modulation following short-term exposure to endocrine disruptors

Rotchell, Jeanette; Cubero-Leon, Elena; Puinean, A. Mirel; Labadie, Pierre; Ciocan, Corina; Itoh, Naoki; Kishida, Mitsuyo; Osada, Makoto; Minier, Christophe; Hill, Elizabeth M.; Rotchell, Jeanette M.

doi:10.1016/j.marenvres.2011.11.012

Two CYP3A-like genes in the marine mussel Mytilus edulis: mRNA expression modulation following short-term exposure to endocrine disruptors

Rotchell, Jeanette; Cubero-Leon, Elena; Puinean, A. Mirel; Labadie, Pierre; Ciocan, Corina; Itoh, Naoki; Kishida, Mitsuyo; Osada, Makoto; Minier, Christophe; Hill, Elizabeth M.; Rotchell, Jeanette M.

Authors

Jeanette Rotchell

Elena Cubero-Leon

A. Mirel Puinean

Pierre Labadie

Corina Ciocan

Naoki Itoh

Mitsuyo Kishida

Makoto Osada

Christophe Minier

Elizabeth M. Hill

Jeanette M. Rotchell

Abstract

Members of the vertebrate CYP3A subfamily are involved in the metabolism of steroids and a wide range of xenobiotics. In this study two CYP3A- like mRNAs have been isolated from the mussel (Mytilus edulis), and their seasonal expression profile and modulation by estrogens examined. Sexual dimorphism of CYP3A- like mRNA expression was not observed in mussel gonads of individuals collected throughout a year. Nevertheless, natural variation in gonadal CYP3A- like mRNA expression was observed, with highest levels of CYP3A isoform1 and lowest levels of CYP3A isoform2 mRNA during the maturation and spawning season. Exposure to a 10% sewage treatment works extract did not result in any significant changes in mRNA expression of CYP3A-like. In contrast, exposure to E2 (200ng/L) and TBT (100ng/L) significantly down-regulated the expression of CYP3A- like isoform1 but not CYP3A-like isoform2 suggesting differential regulation. Understanding xenobiotic metabolism pathways in aquatic invertebrates.

Citation

Cubero-Leon, E., Puinean, A. M., Labadie, P., Ciocan, C., Itoh, N., Kishida, M., Osada, M., Minier, C., Hill, E. M., & Rotchell, J. M. (2012). Two CYP3A-like genes in the marine mussel Mytilus edulis: mRNA expression modulation following short-term exposure to endocrine disruptors. Marine environmental research, 74, 32-39. https://doi.org/10.1016/j.marenvres.2011.11.012

Journal Article Type	Article
Acceptance Date	Nov 21, 2011
Online Publication Date	Dec 7, 2012
Publication Date	2012-03
Journal	Marine environmental research
Print ISSN	0141-1136
Publisher	Elsevier
Peer Reviewed	Peer Reviewed
Volume	74
Pages	32-39
DOI	https://doi.org/10.1016/j.marenvres.2011.11.012
Keywords	Pollution; Ecotoxicology; Mussel; CYP3A
Public URL	https://hull-repository.worktribe.com/output/417621
Publisher URL	https://www.sciencedirect.com/science/article/pii/S0141113611001644?via%3Dihub