Abu Saleh Md Moin
Classical and alternate complement factor overexpression in non-obese weight matched women with polycystic ovary syndrome does not correlate with vitamin D
Moin, Abu Saleh Md; Sathyapalan, Thozhukat; Butler, Alexandra E.; Atkin, Stephen L.
Authors
Professor Thozhukat Sathyapalan T.Sathyapalan@hull.ac.uk
Professor of Diabetes, Endocrinology and Metabolism
Alexandra E. Butler
Stephen L. Atkin
Abstract
Introduction: Women with polycystic ovary syndrome (PCOS) exhibit complement factor expression changes that may be obesity-driven rather than an intrinsic facet of PCOS; furthermore, complement changes have been associated with vitamin D deficiency, a common feature of PCOS. Therefore, complement pathway proteins and vitamin D levels may be linked in PCOS. Methods: We measured plasma levels of complement pathway proteins by Slow Off-rate Modified Aptamer (SOMA)-scan plasma protein measurement for the classical (C4, C4a, and C4b) and alternative pathways (C3, C3b, iC3b, properdin, and factors B, D, and H) in weight and age-matched non-obese non-insulin resistant women with PCOS (n = 24) and control women (n = 24). Proteins that differed between groups were correlated with 25-hydroxyvitamin D3 (25(OH)D3) and 1,25-dihydroxyvitamin D3 (1,25(OH)2D3), measured by isotope-dilution liquid chromatography tandem mass spectrometry. Results: Women with PCOS had a higher free androgen index and anti-Mullerian hormone, though insulin resistance was comparable to controls; likewise, C-reactive protein, a marker of inflammation, was comparable between cohorts. In the alternative complement pathway, C3, iC3b, and properdin were increased in PCOS (p <0.05), while C4 in the classical pathway was increased (p <0.05). 25(OH)D3 levels positively correlated with C3b only in control subjects, with no correlation of 1,25(OH)2D3 with any of the proteins. Conclusion: In a non-obese PCOS population matched for age, insulin resistance and inflammation, initiating proteins of the classical and alternate complement cascades were increased. However, a positive correlation with 25(OH)D3 was only seen for C3b in control subjects, with no correlation to 1,25(OH)2D3, suggesting that the increase in complement proteins in PCOS is vitamin D-independent.
Citation
Moin, A. S. M., Sathyapalan, T., Butler, A. E., & Atkin, S. L. (2022). Classical and alternate complement factor overexpression in non-obese weight matched women with polycystic ovary syndrome does not correlate with vitamin D. Frontiers in endocrinology, 13, Article 935750. https://doi.org/10.3389/fendo.2022.935750
Journal Article Type | Article |
---|---|
Acceptance Date | Dec 7, 2022 |
Online Publication Date | Dec 21, 2022 |
Publication Date | Dec 21, 2022 |
Deposit Date | Feb 18, 2023 |
Publicly Available Date | Feb 20, 2023 |
Journal | Frontiers in Endocrinology |
Print ISSN | 1664-2392 |
Publisher | Frontiers Media |
Peer Reviewed | Peer Reviewed |
Volume | 13 |
Article Number | 935750 |
DOI | https://doi.org/10.3389/fendo.2022.935750 |
Keywords | Polycystic ovary syndrome; Complement factors; C3; C4; vitamin D |
Public URL | https://hull-repository.worktribe.com/output/4176396 |
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Copyright © 2022 Moin, Sathyapalan, Butler and Atkin. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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