Nanoarchitectonics of the aggregation-induced emission luminescent molecule Tetraphenylethylene-COOH (TPE-COOH): Fluorescence imaging of targeted cancer microsphere cells

Luo, Fang; Shen, Lingyi; Zhang, Jinjuan; Huang, Yali; Chen, Tengxiang; Xu, Hong; Zhang, Qilong; Redshaw, Carl; Feng, Xing

Abstract

Cancer microspheres are an ideal cellular model for studying cancer stem cells. However, the lack of effective and rapid fluorescent imaging reagents for cancer microspheres has severely hampered cytological studies associated with cancer microspheres. Here, we have identified an aggregation-induced emission (AIE) probe, TPE-COOH, to bind to a targeting cyclic heptapeptide molecule to form a new TPE-Peptide probe. This probe has been employed for fluorescence imaging of normal, cancer and cancer microsphere cells. A novel analogue of TPAPy-1 was synthesized to verify the cell-specific targeting. For the first time, the fluorescence imaging of cancer microspheres was achieved and microspheres were imaged due to changes in viscosity, thereby providing a means to visualize and identify cancer microspheres.

Citation

Luo, F., Shen, L., Zhang, J., Huang, Y., Chen, T., Xu, H., …Feng, X. (2023). Nanoarchitectonics of the aggregation-induced emission luminescent molecule Tetraphenylethylene-COOH (TPE-COOH): Fluorescence imaging of targeted cancer microsphere cells. Dyes and pigments : an international journal, 214, Article 111192. https://doi.org/10.1016/j.dyepig.2023.111192

• Journal Article Type: Article
• Acceptance Date: Feb 26, 2023
• Online Publication Date: Feb 27, 2023
• Publication Date: Jun 1, 2023
• Deposit Date: Feb 28, 2023
• Publicly Available Date: Feb 28, 2024
• Journal: Dyes and Pigments
• Print ISSN: 0143-7208
• Electronic ISSN: 1873-3743
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 214
• Article Number: 111192
• DOI: https://doi.org/10.1016/j.dyepig.2023.111192
• Keywords: Process Chemistry and Technology; General Chemical Engineering
• Public URL: https://hull-repository.worktribe.com/output/4226642