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Functional artificial free-standing yeast biofilms

Konnova, Svetlana A.; Kahraman, Mehmet; Zamaleeva, Alsu I.; Culha, Mustafa; Paunov, Vesselin N.; Fakhrullin, Rawil F.

Authors

Svetlana A. Konnova

Mehmet Kahraman

Alsu I. Zamaleeva

Mustafa Culha

Rawil F. Fakhrullin



Abstract

Here we report fabrication of artificial free-standing yeast biofilms built using sacrificial calcium carbonate-coated templates and layer-by-layer assembly of extracellular matrix-mimicking polyelectro lyte multilayers. The free-standing biofilms are freely floating multilayered films of oppositely charged polyelectrolytes and live cells incorporated in the polyelectrolyte layers. Such biofilms were initially formed on glass substrates of circular and ribbon-like shapes coated with thin layers of calcium carbonate microparticles. The templates were then coated with cationic and anionic polyelectrolytes to produce a supporting multilayered thin film. Then the yeast alone or mixed with various micro- and nanoparticle inclusions was deposited onto the multilayer composite films and further coated with outer polyelectrolyte multilayers. To detach the biofilms from the glass substrates the calcium carbonate layer was chemically dissolved yielding free-standing composite biofilms. These artificial biofilms to a certain degree mimic the primitive multicellular and colonial species. We have demonstrated the added functionality of the free-standing artificial biofilms containing magnetic, latex and silver micro- and nanoparticles. We have also developed "symbiotic" multicellular biofilms containing yeast and bacteria. This approach for fabrication of free-standing artificial biofilms can be potentially helpful in development of artificial colonial microorganisms composed of several different unicellular species and an important tool for growing cell cultures free of supporting substrates.

Journal Article Type Article
Publication Date Dec 1, 2011
Journal COLLOIDS AND SURFACES B-BIOINTERFACES
Print ISSN 0927-7765
Electronic ISSN 1873-4367
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 88
Issue 2
Pages 656-663
APA6 Citation Konnova, S. A., Kahraman, M., Zamaleeva, A. I., Culha, M., Paunov, V. N., & Fakhrullin, R. F. (2011). Functional artificial free-standing yeast biofilms. Colloids and surfaces. B, Biointerfaces, 88(2), 656-663. https://doi.org/10.1016/j.colsurfb.2011.07.056
DOI https://doi.org/10.1016/j.colsurfb.2011.07.056
Keywords Artificial biofilms; Multicellular clusters; Polyelectrolytes; Layer-by-layer assembly; Viability
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