Regulation of photosynthetic electron flow on dark to light transition by ferredoxin:Nadp(h) oxidoreductase interactions

Kramer, Manuela; Rodriguez-Heredia, Melvin; Saccon, Francesco; Mosebach, Laura; Twachtmann, Manuel; Krieger-Liszkay, Anja; Duffy, Chris; Knell, Robert J.; Finazzi, Giovanni; Hanke, Guy Thomas

Abstract

During photosynthesis, electron transport is necessary for carbon assimilation and must be regulated to minimize free radical damage. There is a longstanding controversy over the role of a critical enzyme in this process (ferredoxin:NADP(H) oxidoreductase, or FNR), and in particular its location within chloroplasts. Here we use immunogold labelling to prove that FNR previously assigned as soluble is in fact membrane associated. We combined this technique with a genetic approach in the model plant Arabidopsis to show that the distribution of this enzyme between different membrane regions depends on its interaction with specific tether proteins. We further demonstrate a correlation between the interaction of FNR with different proteins and the activity of alternative photosynthetic electron transport pathways. This supports a role for FNR location in regulating photosynthetic electron flow during the transition from dark to light.

Citation

Kramer, M., Rodriguez-Heredia, M., Saccon, F., Mosebach, L., Twachtmann, M., Krieger-Liszkay, A., Duffy, C., Knell, R. J., Finazzi, G., & Hanke, G. T. (2021). Regulation of photosynthetic electron flow on dark to light transition by ferredoxin:Nadp(h) oxidoreductase interactions. eLife, 10, Article e56088. https://doi.org/10.7554/eLife.56088

• Journal Article Type: Article
• Acceptance Date: Feb 25, 2021
• Online Publication Date: Mar 9, 2021
• Publication Date: Mar 22, 2021
• Deposit Date: Jul 9, 2023
• Publicly Available Date: Jul 28, 2023
• Journal: eLife
• Electronic ISSN: 2050-084X
• Publisher: eLife Sciences Publications
• Peer Reviewed: Peer Reviewed
• Volume: 10
• Article Number: e56088
• DOI: https://doi.org/10.7554/eLife.56088
• Public URL: https://hull-repository.worktribe.com/output/4330619