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Regulation of photosynthetic electron flow on dark to light transition by ferredoxin:Nadp(h) oxidoreductase interactions

Kramer, Manuela; Rodriguez-Heredia, Melvin; Saccon, Francesco; Mosebach, Laura; Twachtmann, Manuel; Krieger-Liszkay, Anja; Duffy, Chris; Knell, Robert J.; Finazzi, Giovanni; Hanke, Guy Thomas

Authors

Manuela Kramer

Melvin Rodriguez-Heredia

Francesco Saccon

Laura Mosebach

Manuel Twachtmann

Anja Krieger-Liszkay

Chris Duffy

Giovanni Finazzi

Guy Thomas Hanke



Abstract

During photosynthesis, electron transport is necessary for carbon assimilation and must be regulated to minimize free radical damage. There is a longstanding controversy over the role of a critical enzyme in this process (ferredoxin:NADP(H) oxidoreductase, or FNR), and in particular its location within chloroplasts. Here we use immunogold labelling to prove that FNR previously assigned as soluble is in fact membrane associated. We combined this technique with a genetic approach in the model plant Arabidopsis to show that the distribution of this enzyme between different membrane regions depends on its interaction with specific tether proteins. We further demonstrate a correlation between the interaction of FNR with different proteins and the activity of alternative photosynthetic electron transport pathways. This supports a role for FNR location in regulating photosynthetic electron flow during the transition from dark to light.

Citation

Kramer, M., Rodriguez-Heredia, M., Saccon, F., Mosebach, L., Twachtmann, M., Krieger-Liszkay, A., …Hanke, G. T. (2021). Regulation of photosynthetic electron flow on dark to light transition by ferredoxin:Nadp(h) oxidoreductase interactions. eLife, 10, Article e56088. https://doi.org/10.7554/eLife.56088

Journal Article Type Article
Acceptance Date Feb 25, 2021
Online Publication Date Mar 9, 2021
Publication Date Mar 22, 2021
Deposit Date Jul 9, 2023
Publicly Available Date Jul 28, 2023
Journal eLife
Electronic ISSN 2050-084X
Publisher eLife Sciences Publications
Peer Reviewed Peer Reviewed
Volume 10
Article Number e56088
DOI https://doi.org/10.7554/eLife.56088
Public URL https://hull-repository.worktribe.com/output/4330619

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Publisher Licence URL
http://creativecommons.org/licenses/by/4.0

Copyright Statement
Copyright Kramer et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.




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