Skip to main content

Research Repository

Advanced Search

Drosophila Primary Neuronal Cultures as a Useful Cellular Model to Study and Image Axonal Transport

Voelzmann, André; Sanchez-Soriano, Natalia

Authors

Natalia Sanchez-Soriano



Contributors

Alessio Vagnoni
Editor

Abstract

The use of primary neuronal cultures generated from Drosophila tissue provides a powerful model for studies of transport mechanisms. Cultured fly neurons provide similarly detailed subcellular resolution and applicability of pharmacology or fluorescent dyes as mammalian primary neurons. As an experimental advantage for the mechanistic dissection of transport, fly primary neurons can be combined with the fast and highly efficient combinatorial genetics of Drosophila, and genetic tools for the manipulation of virtually every fly gene are readily available. This strategy can be performed in parallel to in vivo transport studies to address relevance of any findings. Here we will describe the generation of primary neuronal cultures from Drosophila embryos and larvae, the use of external fluorescent dyes and genetic tools to label cargo, and the key strategies for live imaging and subsequent analysis.

Citation

Voelzmann, A., & Sanchez-Soriano, N. (2022). Drosophila Primary Neuronal Cultures as a Useful Cellular Model to Study and Image Axonal Transport. In A. Vagnoni (Ed.), Axonal Transport (429-449). New York: Humana Press. https://doi.org/10.1007/978-1-0716-1990-2_23

Online Publication Date Apr 13, 2022
Publication Date Apr 13, 2022
Deposit Date Feb 8, 2024
Publicly Available Date Feb 22, 2024
Publisher Humana Press
Pages 429-449
Series Title Methods in Molecular Biology
Series Number 2431
Series ISSN 1064-3745 ; 1940-6029
Book Title Axonal Transport
ISBN 9781071619896
DOI https://doi.org/10.1007/978-1-0716-1990-2_23
Keywords Live imaging; Axonal transport; Drosophila primary neurons; Motors; Organelles; Mitochondria; Kinesin; Dynein; Lysotracker; Mitotracker
Public URL https://hull-repository.worktribe.com/output/4371931

Files

Chapter (719 Kb)
PDF

Publisher Licence URL
http://creativecommons.org/licenses/by/4.0

Copyright Statement
© The Author(s) 2022.
Open Access This chapter is licensed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

The images or other third party material in this chapter are included in the chapter's Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the chapter's Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.




You might also like



Downloadable Citations