Dr Andre Voelzmann A.Voelzmann@hull.ac.uk
Lecturer in Cell Biology & Biotechnology
Drosophila Primary Neuronal Cultures as a Useful Cellular Model to Study and Image Axonal Transport
Voelzmann, André; Sanchez-Soriano, Natalia
Authors
Natalia Sanchez-Soriano
Contributors
Alessio Vagnoni
Editor
Abstract
The use of primary neuronal cultures generated from Drosophila tissue provides a powerful model for studies of transport mechanisms. Cultured fly neurons provide similarly detailed subcellular resolution and applicability of pharmacology or fluorescent dyes as mammalian primary neurons. As an experimental advantage for the mechanistic dissection of transport, fly primary neurons can be combined with the fast and highly efficient combinatorial genetics of Drosophila, and genetic tools for the manipulation of virtually every fly gene are readily available. This strategy can be performed in parallel to in vivo transport studies to address relevance of any findings. Here we will describe the generation of primary neuronal cultures from Drosophila embryos and larvae, the use of external fluorescent dyes and genetic tools to label cargo, and the key strategies for live imaging and subsequent analysis.
Citation
Voelzmann, A., & Sanchez-Soriano, N. (2022). Drosophila Primary Neuronal Cultures as a Useful Cellular Model to Study and Image Axonal Transport. In A. Vagnoni (Ed.), Axonal Transport (429-449). New York: Humana Press. https://doi.org/10.1007/978-1-0716-1990-2_23
Online Publication Date | Apr 13, 2022 |
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Publication Date | Apr 13, 2022 |
Deposit Date | Feb 8, 2024 |
Publicly Available Date | Feb 22, 2024 |
Publisher | Humana Press |
Pages | 429-449 |
Series Title | Methods in Molecular Biology |
Series Number | 2431 |
Series ISSN | 1064-3745 ; 1940-6029 |
Book Title | Axonal Transport |
ISBN | 9781071619896 |
DOI | https://doi.org/10.1007/978-1-0716-1990-2_23 |
Keywords | Live imaging; Axonal transport; Drosophila primary neurons; Motors; Organelles; Mitochondria; Kinesin; Dynein; Lysotracker; Mitotracker |
Public URL | https://hull-repository.worktribe.com/output/4371931 |
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© The Author(s) 2022.
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