Molecular changes in skin pigmented lesions of the coral trout Plectropomus leopardus

Lerebours, Adelaide; Chapman, Emma C.; Rotchell, Jeanette M.; Heupel, Michelle R.; Sweet, Michael J.

Abstract

© 2016 Elsevier Ltd A high prevalence of skin pigmented lesions of 15% was recently reported in coral trout Plectropomus leopardus, a commercially important marine fish, inhabiting the Great Barrier Reef. Herein, fish were sampled at two offshore sites, characterised by high and low lesion prevalence. A transcriptomic approach using the suppressive subtractive hybridisation (SSH) method was used to analyse the differentially expressed genes between lesion and normal skin samples. Transcriptional changes of 14 genes were observed in lesion samples relative to normal skin samples. These targeted genes encoded for specific proteins which are involved in general cell function but also in different stages disrupted during the tumourigenesis process of other organisms, such as cell cycling, cell proliferation, skeletal organisation and cell migration. The results highlight transcripts that are associated with the lesion occurrence, contributing to a better understanding of the molecular aetiology of this coral trout skin disease.

Citation

Lerebours, A., Chapman, E. C., Rotchell, J. M., Heupel, M. R., & Sweet, M. J. (2016). Molecular changes in skin pigmented lesions of the coral trout Plectropomus leopardus. Marine environmental research, 120, 130-135. https://doi.org/10.1016/j.marenvres.2016.07.009

• Journal Article Type: Article
• Acceptance Date: Jul 16, 2016
• Online Publication Date: Jul 28, 2016
• Publication Date: Sep 1, 2016
• Deposit Date: Jul 21, 2016
• Publicly Available Date: Jul 28, 2016
• Journal: Marine environmental research
• Print ISSN: 0141-1136
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 120
• Pages: 130-135
• DOI: https://doi.org/10.1016/j.marenvres.2016.07.009
• Keywords: Fish, Skin lesion, Melanosis, Gene expression
• Public URL: https://hull-repository.worktribe.com/output/441515
• Publisher URL: http://www.sciencedirect.com/science/article/pii/S0141113616301271
• Additional Information: This article is maintained by: Elsevier; Article Title: Molecular changes in skin pigmented lesions of the coral trout Plectropomus leopardus; Journal Title: Marine Environmental Research; CrossRef DOI link to publisher maintained version: http://dx.doi.org/10.1016/j.marenvres.2016.07.009; Content Type: article; Copyright: © 2016 Elsevier Ltd. All rights reserved.
• Contract Date: Jul 21, 2016