Enhanced antimicrobial effect of berberine in nanogel carriers with cationic surface functionality

Al-Awady, Mohammed J.; Fauchet, Adelaide; Greenway, Gillian M.; Paunov, Vesselin N.

Abstract

We report a strong enhancement in the antimicrobial action of berberine encapsulated into polyacrylic acid-based nanogels followed by further surface functionalisation with a cationic polyelectrolyte (PDAC). Due to the highly developed surface area, the nanogel carrier amplifies the contact of berberine with microbial cells and increases its antimicrobial efficiency. We show that such cationic nanogel carriers of berberine can adhere directly to the cell membranes and maintain a very high concentration of berberine directly on the cell surface. We demonstrated that the antimicrobial action of the PDAC-coated nanogel loaded with berberine on E. coli and C. reinhardtii is much higher than that of the equivalent solution of free berberine due to the electrostatic adhesion between the positively charged nanogel particles and the cell membranes. Our results also showed a marked increase in their antimicrobial action at shorter incubation times compared to the non-coated nanogel particles loaded with berberine under the same conditions. We attribute this boost in the antimicrobial effect of these cationic nanocarriers to their accumulation on the cell membranes which sustains a high concentration of released berberine causing cell death within much shorter incubation times. This study can provide a blueprint for boosting the action of other cationic antimicrobial agents by encapsulating them into nanogel carriers functionalised with a cationic surface layer. This nanotechnology-based approach could lead to the development of more effective wound dressings, disinfecting agents, antimicrobial surfaces, and antiseptic and antialgal/antibiofouling formulations.

Citation

Al-Awady, M. J., Fauchet, A., Greenway, G. M., & Paunov, V. N. (2017). Enhanced antimicrobial effect of berberine in nanogel carriers with cationic surface functionality. Journal of Materials Chemistry B, 5(38), 7885-7897. https://doi.org/10.1039/c7tb02262j

• Acceptance Date: Sep 14, 2017
• Online Publication Date: Sep 26, 2017
• Publication Date: Sep 26, 2017
• Deposit Date: Oct 9, 2017
• Publicly Available Date: Sep 27, 2018
• Journal: Journal of materials chemistry B
• Print ISSN: 2050-750X
• Electronic ISSN: 2050-7518
• Publisher: Royal Society of Chemistry
• Peer Reviewed: Peer Reviewed
• Volume: 5
• Issue: 38
• Pages: 7885-7897
• DOI: https://doi.org/10.1039/c7tb02262j
• Keywords: General Materials Science; General Chemistry; Biomedical Engineering; General Medicine
• Public URL: https://hull-repository.worktribe.com/output/455463
• Publisher URL: http://pubs.rsc.org/en/content/articlelanding/2017/tb/c7tb02262j#!divAbstract
• Additional Information: This is the accepted manuscript of an article published in Journal of materials chemistry B, 2017. The version of record is available at the DOI link in this record.