Dopamine induces functional extracellular traps in microglia

Agrawal, Ishan; Sharma, Nidhi; Saxena, Shivanjali; Arvind, S.; Chakraborty, Debayani; Chakraborty, Debarati Bhunia; Jha, Deepak; Ghatak, Surajit; Epari, Sridhar; Gupta, Tejpal; Jha, Sushmita

Abstract

Dopamine (DA) plays many roles in the brain, especially in movement, motivation, and reinforcement of behavior; however, its role in regulating innate immunity is not clear. Here, we show that DA can induce DNA-based extracellular traps in primary, adult, human microglia and BV2 microglia cell line. These DNA-based extracellular traps are formed independent of reactive oxygen species, actin polymerization, and cell death. These traps are functional and capture fluorescein (FITC)-tagged Escherichia coli even when reactive oxygen species production or actin polymerization is inhibited. We show that microglial extracellular traps are present in Glioblastoma multiforme. This is crucial because Glioblastoma multiforme cells are known to secrete DA. Our findings demonstrate that DA plays a significant role in sterile neuro-inflammation by inducing microglia extracellular traps.

Citation

Agrawal, I., Sharma, N., Saxena, S., Arvind, S., Chakraborty, D., Chakraborty, D. B., Jha, D., Ghatak, S., Epari, S., Gupta, T., & Jha, S. (2021). Dopamine induces functional extracellular traps in microglia. iScience, 24(1), Article 101968. https://doi.org/10.1016/j.isci.2020.101968

• Journal Article Type: Article
• Acceptance Date: Nov 16, 2020
• Online Publication Date: Jan 6, 2021
• Publication Date: Jan 22, 2021
• Deposit Date: Mar 13, 2024
• Publicly Available Date: Mar 13, 2024
• Journal: iScience
• Print ISSN: 2589-0042
• Electronic ISSN: 2589-0042
• Publisher: Cell Press
• Peer Reviewed: Peer Reviewed
• Volume: 24
• Issue: 1
• Article Number: 101968
• DOI: https://doi.org/10.1016/j.isci.2020.101968
• Keywords: Molecular biology; Cellular neuroscience; Immunology; Cell biology
• Public URL: https://hull-repository.worktribe.com/output/4588967