Characterization of cellular chemical dynamics using combined microfluidic and Raman techniques

Zhang, Xunli; Yin, Huabing; Cooper, Jon M.; Haswell, Stephen J.

Abstract

The integration of a range of technologies including microfluidics, surface-enhanced Raman scattering and confocal microspectroscopy has been successfully used to characterize in situ single living CHO (Chinese hamster ovary) cells with a high degree of spatial (in three dimensions) and temporal (1 s per spectrum) resolution. Following the introduction of a continuous flow of ionomycin, the real time spectral response from the cell was monitored during the agonist-evoked Ca2+ flux process. The methodology described has the potential to be used for the study of the cellular dynamics of a range of signalling processes.

Citation

Zhang, X., Yin, H., Cooper, J. M., & Haswell, S. J. (2008). Characterization of cellular chemical dynamics using combined microfluidic and Raman techniques. Analytical and Bioanalytical Chemistry, 390(3), 833-840. https://doi.org/10.1007/s00216-007-1564-9

• Journal Article Type: Article
• Acceptance Date: Aug 17, 2007
• Online Publication Date: Sep 12, 2007
• Publication Date: 2008-02
• Deposit Date: Nov 13, 2014
• Publicly Available Date: Nov 13, 2014
• Journal: Analytical and bioanalytical chemistry
• Print ISSN: 1618-2642
• Electronic ISSN: 1618-2650
• Publisher: Springer Verlag
• Peer Reviewed: Peer Reviewed
• Volume: 390
• Issue: 3
• Pages: 833-840
• DOI: https://doi.org/10.1007/s00216-007-1564-9
• Keywords: Microfluidics, Chinese hamster ovary (CHO) cells, Confocal microspectroscopy, Surface-enhanced Raman scattering (SERS), Dynamic monitoring
• Public URL: https://hull-repository.worktribe.com/output/460327
• Publisher URL: http://link.springer.com/article/10.1007%2Fs00216-007-1564-9
• Additional Information: Copy of article first published in: Analytical and bioanalytical chemistry, 2008, v.390, issue 3.