Synthesis of a novel HER2 targeted aza-BODIPY–antibody conjugate: synthesis, photophysical characterisation and in vitro evaluation

Cheng, Miffy. H. Y.; Cheng, Miffy H.Y.; Maruani, Antoine; Savoie, Huguette; Chudasama, Vijay; Boyle, Ross. W.

Abstract

We herein report the synthesis and analysis of a novel aza-BODIPY–antibody conjugate, formed by controlled and regioselective bioconjugation methodology. Employing the clinically relevant antibody, which targets HER2 positive cancers, represents the first example of an antibody targeting strategy for this class of near-IR emitting fluorophore. The NIR fluorescence and binding properties were validated through in vitro studies using live cell confocal imaging.

Citation

Cheng, M. H. Y., Cheng, M. H., Maruani, A., Savoie, H., Chudasama, V., & Boyle, R. W. (2018). Synthesis of a novel HER2 targeted aza-BODIPY–antibody conjugate: synthesis, photophysical characterisation and in vitro evaluation. Organic & biomolecular chemistry, 16(7), 1144-1149. https://doi.org/10.1039/c7ob02957h

• Journal Article Type: Article
• Acceptance Date: Jan 16, 2018
• Publication Date: Jan 16, 2018
• Deposit Date: Jan 17, 2018
• Publicly Available Date: Jan 17, 2019
• Journal: Organic & biomolecular chemistry
• Print ISSN: 1477-0520
• Publisher: Royal Society of Chemistry
• Peer Reviewed: Peer Reviewed
• Volume: 16
• Issue: 7
• Pages: 1144-1149
• DOI: https://doi.org/10.1039/c7ob02957h
• Keywords: Physical and Theoretical Chemistry; Organic Chemistry; Biochemistry
• Public URL: https://hull-repository.worktribe.com/output/546953
• Publisher URL: http://pubs.rsc.org/en/content/articlelanding/2018/ob/c7ob02957h#!divAbstract
• Additional Information: : This document is Similarity Check deposited; : Supplementary Information; : Antoine Maruani (ORCID); : Antoine Maruani (ResearcherID); : Vijay Chudasama (ORCID); : Ross. W. Boyle (ORCID); : Single-blind; : Received 30 November 2017; Accepted 16 January 2018; Accepted Manuscript published 16 January 2018; Advance Article published 24 January 2018; Version of Record published 14 February 2018
• Contract Date: Jan 17, 2018