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Detection of a new non-native freshwater species by DNA metabarcoding of environmental samples — first record of Gammarus fossarum in the UK

Blackman, Rosetta C.; Constable, Drew; Hahn, Christoph; Sheard, Andrew M.; Durkota, Jessica; Hänfling, Bernd; Handley, Lori Lawson

Authors

Rosetta C. Blackman

Drew Constable

Christoph Hahn

Andrew M. Sheard

Jessica Durkota

Dr Bernd Haenfling B.Haenfling@hull.ac.uk
Senior Lecturer/ Chair of the Evolutionary Biology Group (EvoHull)

Lori Lawson Handley



Abstract

© 2017 The Author(s). and 2017 REABIC. We report the discovery of a non-native gammarid, Gammarus fossarum (Koch, 1836) (Crustacea, Amphipoda), in UK rivers. Gammarus fossarum is a common freshwater gammarid in many parts of mainland Europe, but was previously considered absent from the UK. Gammarus fossarum was detected in a number of UK rivers following DNA metabarcoding of a mini-barcode region of the COI gene in macroinvertebrate kick samples, and environmental DNA (eDNA) from water and sediment samples. Subsequent morphological analysis and standard DNA barcoding showed that the species could be reliably identified and separated from Gammarus pulex (Linnaeus, 1758), the most dominant and widespread native freshwater gammarid in the UK. Our data demonstrate extensive geographical coverage of G. fossarum in the UK, spanning distant river catchments. At present there is no data to confirm the likely origin of G. fossarum’s introduction. Subsequent re-examination of historic archive material shows the species to have been present in the UK since at least 1964. This study is among the first to demonstrate the potential of eDNA metabarcoding for detection of new non-native species.

Citation

Blackman, R. C., Constable, D., Hahn, C., Sheard, A. M., Durkota, J., Hänfling, B., & Handley, L. L. (2017). Detection of a new non-native freshwater species by DNA metabarcoding of environmental samples — first record of Gammarus fossarum in the UK. Aquatic Invasions, 12(2), 177-189. https://doi.org/10.3391/ai.2017.12.2.06

Journal Article Type Article
Acceptance Date Mar 20, 2017
Online Publication Date Apr 24, 2017
Publication Date Jun 1, 2017
Deposit Date Jul 5, 2018
Publicly Available Date Jul 10, 2018
Journal Aquatic Invasions
Print ISSN 1798-6540
Electronic ISSN 1818-5487
Peer Reviewed Peer Reviewed
Volume 12
Issue 2
Pages 177-189
DOI https://doi.org/10.3391/ai.2017.12.2.06
Keywords Environmental DNA; Metabarcoding; Passive detection; Early warning; Cryptic species; Gammaridae; Non-native
Public URL https://hull-repository.worktribe.com/output/912837
Publisher URL http://www.aquaticinvasions.net/2017/issue2.html
Copyright Statement © 2017 The Author(s). Journal compilation © 2017 REABIC

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Copyright Statement
© 2017 The Author(s). Journal compilation © 2017 REABIC





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