Circadian oscillations of cytosolic free calcium regulate the Arabidopsis circadian clock

Martí Ruiz, María Carmen; Hubbard, Katharine E.; Gardner, Michael J.; Jung, Hyun Ju; Aubry, Sylvain; Hotta, Carlos T.; Mohd-Noh, Nur Izzati; Robertson, Fiona C.; Hearn, Timothy J.; Tsai, Yu-Chang; Dodd, Antony N.; Hannah, Matthew; Carré, Isabelle A.; Davies, Julia M.; Braam, Janet; Webb, Alex A.R.

Abstract

In the last decade, the view of circadian oscillators has expanded from transcriptional feedback to incorporate post-transcriptional, post-translational, metabolic processes and ionic signalling. In plants and animals, there are circadian oscillations in the concentration of cytosolic free Ca2+ ([Ca2+]cyt), though their purpose has not been fully characterized. We investigated whether circadian oscillations of [Ca2+]cyt regulate the circadian oscillator of Arabidopsis thaliana. We report that in Arabidopsis, [Ca2+]cyt circadian oscillations can regulate circadian clock function through the Ca2+-dependent action of CALMODULIN-LIKE24 (CML24). Genetic analyses demonstrate a linkage between CML24 and the circadian oscillator, through pathways involving the circadian oscillator gene TIMING OF CAB2 EXPRESSION1 (TOC1).

Citation

Martí Ruiz, M. C., Hubbard, K. E., Gardner, M. J., Jung, H. J., Aubry, S., Hotta, C. T., Mohd-Noh, N. I., Robertson, F. C., Hearn, T. J., Tsai, Y.-C., Dodd, A. N., Hannah, M., Carré, I. A., Davies, J. M., Braam, J., & Webb, A. A. (2018). Circadian oscillations of cytosolic free calcium regulate the Arabidopsis circadian clock. Nature Plants, 4(9), 690-698. https://doi.org/10.1038/s41477-018-0224-8

• Journal Article Type: Article
• Acceptance Date: Jul 17, 2018
• Online Publication Date: Aug 20, 2018
• Publication Date: Sep 1, 2018
• Deposit Date: Oct 3, 2018
• Publicly Available Date: Feb 21, 2019
• Journal: Nature Plants
• Print ISSN: 2055-0278
• Publisher: Nature Publishing Group
• Peer Reviewed: Peer Reviewed
• Volume: 4
• Issue: 9
• Pages: 690-698
• DOI: https://doi.org/10.1038/s41477-018-0224-8
• Keywords: Circadian rhythms in plants; Plant signalling
• Public URL: https://hull-repository.worktribe.com/output/989681
• Publisher URL: https://www.nature.com/articles/s41477-018-0224-8
• Related Public URLs: https://www.repository.cam.ac.uk/handle/1810/280204
• Contract Date: Oct 4, 2018