Circadian oscillations of cytosolic free calcium regulate the Arabidopsis circadian clock

Martí Ruiz, María Carmen; Hubbard, Katharine E.; Gardner, Michael J.; Jung, Hyun Ju; Aubry, Sylvain; Hotta, Carlos T.; Mohd-Noh, Nur Izzati; Robertson, Fiona C.; Hearn, Timothy J.; Tsai, Yu-Chang; Dodd, Antony N.; Hannah, Matthew; Carré, Isabelle A.; Davies, Julia M.; Braam, Janet; Webb, Alex A.R.

doi:10.1038/s41477-018-0224-8

Circadian oscillations of cytosolic free calcium regulate the Arabidopsis circadian clock

Martí Ruiz, María Carmen; Hubbard, Katharine E.; Gardner, Michael J.; Jung, Hyun Ju; Aubry, Sylvain; Hotta, Carlos T.; Mohd-Noh, Nur Izzati; Robertson, Fiona C.; Hearn, Timothy J.; Tsai, Yu-Chang; Dodd, Antony N.; Hannah, Matthew; Carré, Isabelle A.; Davies, Julia M.; Braam, Janet; Webb, Alex A.R.

Authors

María Carmen Martí Ruiz

Dr Katharine Hubbard K.Hubbard@hull.ac.uk
Reader in Biology Education

Michael J. Gardner

Hyun Ju Jung

Sylvain Aubry

Carlos T. Hotta

Nur Izzati Mohd-Noh

Fiona C. Robertson

Timothy J. Hearn

Yu-Chang Tsai

Antony N. Dodd

Matthew Hannah

Isabelle A. Carré

Julia M. Davies

Janet Braam

Alex A.R. Webb

Abstract

In the last decade, the view of circadian oscillators has expanded from transcriptional feedback to incorporate post-transcriptional, post-translational, metabolic processes and ionic signalling. In plants and animals, there are circadian oscillations in the concentration of cytosolic free Ca2+ ([Ca2+]cyt), though their purpose has not been fully characterized. We investigated whether circadian oscillations of [Ca2+]cyt regulate the circadian oscillator of Arabidopsis thaliana. We report that in Arabidopsis, [Ca2+]cyt circadian oscillations can regulate circadian clock function through the Ca2+-dependent action of CALMODULIN-LIKE24 (CML24). Genetic analyses demonstrate a linkage between CML24 and the circadian oscillator, through pathways involving the circadian oscillator gene TIMING OF CAB2 EXPRESSION1 (TOC1).

Citation

Martí Ruiz, M. C., Hubbard, K. E., Gardner, M. J., Jung, H. J., Aubry, S., Hotta, C. T., …Webb, A. A. (2018). Circadian oscillations of cytosolic free calcium regulate the Arabidopsis circadian clock. Nature Plants, 4(9), 690-698. https://doi.org/10.1038/s41477-018-0224-8

Journal Article Type	Article
Acceptance Date	Jul 17, 2018
Online Publication Date	Aug 20, 2018
Publication Date	Sep 1, 2018
Deposit Date	Oct 3, 2018
Publicly Available Date	Oct 27, 2022
Journal	Nature Plants
Print ISSN	2055-0278
Electronic ISSN	2055-0278
Publisher	Nature Publishing Group
Peer Reviewed	Peer Reviewed
Volume	4
Issue	9
Pages	690-698
DOI	https://doi.org/10.1038/s41477-018-0224-8
Keywords	Circadian rhythms in plants; Plant signalling
Public URL	https://hull-repository.worktribe.com/output/989681
Publisher URL	https://www.nature.com/articles/s41477-018-0224-8
Related Public URLs	https://www.repository.cam.ac.uk/handle/1810/280204