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Alteration in endothelial permeability occurs in response to the activation of PAR2 by factor Xa but not directly by the TF-factor VIIa complex (2019)
Journal Article
Benelhaj, N. E., Maraveyas, A., Featherby, S., Collier, M. E., Johnson, M. J., & Ettelaie, C. (2019). Alteration in endothelial permeability occurs in response to the activation of PAR2 by factor Xa but not directly by the TF-factor VIIa complex. Thrombosis Research, 175, 13-20. https://doi.org/10.1016/j.thromres.2019.01.009

Alterations in the endothelial permeability occur in response to the activation of coagulation mechanisms in order to control clot formation. The activation of the protease activated receptors (PAR) can induce signals that regulate such cellular resp... Read More about Alteration in endothelial permeability occurs in response to the activation of PAR2 by factor Xa but not directly by the TF-factor VIIa complex.

Elucidation of the signalling mechanisms involved in TF-mediated apoptosis in endothelial cells (2018)
Thesis
Ethaeb, A. M. (2018). Elucidation of the signalling mechanisms involved in TF-mediated apoptosis in endothelial cells. (Thesis). University of Hull. Retrieved from https://hull-repository.worktribe.com/output/4220989

Tissue factor (TF) is the main initiator of blood coagulation. In addition to its procoagulant property, TF has the ability to regulate various functions within cells including proliferation, angiogenesis and apoptosis. These outcomes appear to depen... Read More about Elucidation of the signalling mechanisms involved in TF-mediated apoptosis in endothelial cells.

Investigation of the filamin A-Dependent mechanisms of tissue factor incorporation into microvesicles (2017)
Journal Article
Collier, M. E. W., Ettelaie, C., Goult, B. T., Maraveyas, A., & Goodall, A. H. (2017). Investigation of the filamin A-Dependent mechanisms of tissue factor incorporation into microvesicles. Thrombosis and haemostasis, 117(11), 2034-2044. https://doi.org/10.1160/TH17-01-0009

We have previously shown that phosphorylation of tissue factor (TF) at Ser253 increases the incorporation of TF into microvesicles (MVs) following protease-activated receptor 2 (PAR2) activation through a process involving filamin-A, whereas Ser258 p... Read More about Investigation of the filamin A-Dependent mechanisms of tissue factor incorporation into microvesicles.

Tissue factor-bearing microparticles and inflammation: a potential mechanism for the development of venous thromboembolism in cancer (2017)
Journal Article
Date, K., Ettelaie, C., & Maraveyas, A. (2017). Tissue factor-bearing microparticles and inflammation: a potential mechanism for the development of venous thromboembolism in cancer. Journal of thrombosis and haemostasis : JTH, 15(12), 2289-2299. https://doi.org/10.1111/jth.13871

© 2017 International Society on Thrombosis and Haemostasis Summary: Cancer is associated with an increased risk of venous thromboembolism (VTE); the exact mechanisms for the induction of VTE remain to be fully elucidated, but it is widely acknowledge... Read More about Tissue factor-bearing microparticles and inflammation: a potential mechanism for the development of venous thromboembolism in cancer.

Peptidyl-prolyl isomerase 1 (Pin1) preserves the phosphorylation state of tissue factor and prolongs its release within microvesicles (2017)
Journal Article
Ettelaie, C., Collier, M., Featherby, S., Greenman, J., & Maraveyas, A. (2018). Peptidyl-prolyl isomerase 1 (Pin1) preserves the phosphorylation state of tissue factor and prolongs its release within microvesicles. BBA - Molecular Cell Research, 1865(1), 12-24. https://doi.org/10.1016/j.bbamcr.2017.09.016

© 2017 Elsevier B.V. The exposure and release of TF is regulated by post-translational modifications of its cytoplasmic domain. Here, the potential of Pin1 to interact with the cytoplasmic domain of TF, and the outcome on TF function was examined. MD... Read More about Peptidyl-prolyl isomerase 1 (Pin1) preserves the phosphorylation state of tissue factor and prolongs its release within microvesicles.

Identification and functional characterization of putative mitochondrial iron transporters from the human pathogen Trypanosoma brucei (2017)
Thesis
Zheng, F. (2017). Identification and functional characterization of putative mitochondrial iron transporters from the human pathogen Trypanosoma brucei. (Thesis). University of Hull. Retrieved from https://hull-repository.worktribe.com/output/4221817

Trypanosoma brucei is a medically important protozoan parasite causing African sleeping sickness in humans. Treatment of this disease is possible, but is hindered by the limited availability of effective drugs as well as the rapid emergence of drug r... Read More about Identification and functional characterization of putative mitochondrial iron transporters from the human pathogen Trypanosoma brucei.

Oligoubiquitination of tissue factor on Lys255 promotes Ser253-dephosphorylation and terminates TF release (2016)
Journal Article
Ettelaie, C., Collier, M. E., Featherby, S., Greenman, J., & Maraveyas, A. (2016). Oligoubiquitination of tissue factor on Lys255 promotes Ser253-dephosphorylation and terminates TF release. BBA - Molecular Cell Research, 1863(11), 2846-2857. https://doi.org/10.1016/j.bbamcr.2016.09.005

Restriction of tissue factor (TF) activity at the cell surface and TF release are critical for prevention of excessive coagulation. This study examined the regulation of TF dephosphorylation and its release through ubiquitination. A plasmid containin... Read More about Oligoubiquitination of tissue factor on Lys255 promotes Ser253-dephosphorylation and terminates TF release.

Biomarkers of coronary endothelial health: correlation with invasive measures of collateral function, flow and resistance in chronically occluded coronary arteries and the effect of recanalization (2016)
Journal Article
Ladwiniec, A., Ettelaie, C., Cunnington, M. S., Rossington, J., Thackray, S., Alamgir, F., & Hoye, A. (2016). Biomarkers of coronary endothelial health: correlation with invasive measures of collateral function, flow and resistance in chronically occluded coronary arteries and the effect of recanalization. Coronary Artery Disease, 27(4), 287-294. https://doi.org/10.1097/mca.0000000000000360

Objectives: In the presence of a chronically occluded coronary artery, the collateral circulation matures by a process of arteriogenesis; however, there is considerable variation between individuals in the functional capacity of that collateral netwo... Read More about Biomarkers of coronary endothelial health: correlation with invasive measures of collateral function, flow and resistance in chronically occluded coronary arteries and the effect of recanalization.

Analysis of the potential of cancer cell lines to release tissue factor-containing microvesicles: correlation with tissue factor and PAR2 expression (2016)
Journal Article
Ettelaie, C., Collier, M. E. W., Featherby, S., Benelhaj, N. E., Greenman, J., & Maraveyas, A. (2016). Analysis of the potential of cancer cell lines to release tissue factor-containing microvesicles: correlation with tissue factor and PAR2 expression. Thrombosis journal, 14(2), Article ARTN 2. https://doi.org/10.1186/s12959-016-0075-3

Background
Despite the association of cancer-derived circulating tissue factor (TF)-containing microvesicles and hypercoagulable state, correlations with the incidence of thrombosis remain unclear.

Methods
In this study the upregulation of TF re... Read More about Analysis of the potential of cancer cell lines to release tissue factor-containing microvesicles: correlation with tissue factor and PAR2 expression.

Accumulation of tissue factor in endothelial cells induces cell apoptosis, mediated through p38 and p53 activation (2015)
Journal Article
ElKeeb, A. M., Collier, M. E. W., Maraveyas, A., & Ettelaie, C. (2015). Accumulation of tissue factor in endothelial cells induces cell apoptosis, mediated through p38 and p53 activation. Thrombosis and haemostasis, 114(2), 364-378. https://doi.org/10.1160/th14-09-0795

We previously reported that high levels of tissue factor (TF) can induce cellular apoptosis in endothelial. In this study, TF-mediated mechanisms of induction of apoptosis were explored. Endothelial cells were transfected to express wild-type TF. Add... Read More about Accumulation of tissue factor in endothelial cells induces cell apoptosis, mediated through p38 and p53 activation.

Extracellular vesicles, tissue factor, cancer and thrombosis – discussion themes of the ISEV 2014 Educational Day (2015)
Journal Article
Gardiner, C., Harrison, P., Belting, M., Böing, A., Campello, E., Carter, B. S., Collier, M. E., Coumans, F., Ettelaie, C., Van Es, N., Hochberg, F. H., Mackman, N., Rennert, R. C., Thaler, J., Rak, J., & Nieuwland, R. (2015). Extracellular vesicles, tissue factor, cancer and thrombosis – discussion themes of the ISEV 2014 Educational Day. Journal of Extracellular Vesicles, 4(1), 1-14. https://doi.org/10.3402/jev.v4.26901

Although the association between cancer and venous thromboembolism (VTE) has long been known, the mechanisms are poorly understood. Circulating tissue factor-bearing extracellular vesicles have been proposed as a possible explanation for the increase... Read More about Extracellular vesicles, tissue factor, cancer and thrombosis – discussion themes of the ISEV 2014 Educational Day.

An investigation into the mechanisms of tissue factor-mediated apoptosis in endothelial cells (2014)
Thesis
ElKeeb, A. M. (2014). An investigation into the mechanisms of tissue factor-mediated apoptosis in endothelial cells. (Thesis). University of Hull. Retrieved from https://hull-repository.worktribe.com/output/4217145

Cells are known to express and release tissue factor (TF) following activation. In addition, endothelial cells are capable of acquiring TF carried by circulating microvesicles. Accumulation of TF in the endothelium contributes to chronic pathological... Read More about An investigation into the mechanisms of tissue factor-mediated apoptosis in endothelial cells.

Characterization of physical properties of tissue factor-containing microvesicles and a comparison of ultracentrifuge-based recovery procedures (2014)
Journal Article
Ettelaie, C., Collier, M., Maraveyas, A., & Ettelaie, R. (2014). Characterization of physical properties of tissue factor-containing microvesicles and a comparison of ultracentrifuge-based recovery procedures. Journal of Extracellular Vesicles, 3(1), 1-14. https://doi.org/10.3402/jev.v3.23592

© 2014 Camille Ettelaie et al. Microvesicles were isolated from the conditioned media of 3 cell lines (MDA-MB-231, AsPC-1 and A375) by ultracentrifugation at a range of relative centrifugal forces, and the tissue factor (TF) protein and activity, mic... Read More about Characterization of physical properties of tissue factor-containing microvesicles and a comparison of ultracentrifuge-based recovery procedures.

Filamin-A is required for the incorporation of tissue factor into cell-derived microvesicles (2013)
Journal Article
Collier, M. E. W., Ettelaie, C., & Maraveyas, A. (2014). Filamin-A is required for the incorporation of tissue factor into cell-derived microvesicles. Thrombosis and haemostasis, 111(4), 647-655. https://doi.org/10.1160/TH13-09-0769

We previously reported that the incorporation of tissue factor (TF) into cell-derived microvesicles (MVs) is regulated by the phosphorylation of the cytoplasmic domain of TF. Since the cytoskeletal protein filamin-A is known to bind to the cytoplasmi... Read More about Filamin-A is required for the incorporation of tissue factor into cell-derived microvesicles.

Microparticle-associated tissue factor is recycled by endothelial cells resulting in enhanced surface tissue factor activity (2013)
Journal Article
Collier, M. E. W., Mah, P.-M., Xiao, Y., Maraveyas, A., & Ettelaie, C. (2013). Microparticle-associated tissue factor is recycled by endothelial cells resulting in enhanced surface tissue factor activity. Thrombosis and haemostasis, 110(5), 966-976. https://doi.org/10.1160/TH13-01-0055

In this study the uptake of tissue factor (TF)-positive microparticles by endothelial cells and the recycling of the TF component were examined. Human dermal blood endothelial cells (HDBEC) were incubated with microparticles derived from cancer cell... Read More about Microparticle-associated tissue factor is recycled by endothelial cells resulting in enhanced surface tissue factor activity.

p38 alpha phosphorylates serine 258 within the cytoplasmic domain of tissue factor and prevents its incorporation into cell-derived microparticles (2013)
Journal Article
Ettelaie, C., ElKeeb, A. M., Maraveyas, A., & Collier, M. E. W. (2013). p38 alpha phosphorylates serine 258 within the cytoplasmic domain of tissue factor and prevents its incorporation into cell-derived microparticles. BBA - Molecular Cell Research, 1833(3), 613-621. https://doi.org/10.1016/j.bbamcr.2012.11.010

We previously showed that the phosphorylation of Ser253 within the cytoplasmic domain of human tissue factor (TF) initiates the incorporation and release of this protein into cell-derived microparticles. Furthermore, subsequent phosphorylation of Ser... Read More about p38 alpha phosphorylates serine 258 within the cytoplasmic domain of tissue factor and prevents its incorporation into cell-derived microparticles.

Examination of the up-regulation and transfer of tissue factor to endothelial cells by tumour-derived microparticles (2013)
Thesis
Mah, P. M. (2013). Examination of the up-regulation and transfer of tissue factor to endothelial cells by tumour-derived microparticles. (Thesis). University of Hull. Retrieved from https://hull-repository.worktribe.com/output/4215976

Overexpression of tissue factor and its release as microparticles is a common feature of aggressive cancer. Furthermore, increased levels of tissue factor have been detected in the tumour associated microvascular endothelial cells. The accumulation o... Read More about Examination of the up-regulation and transfer of tissue factor to endothelial cells by tumour-derived microparticles.

Low molecular weight heparin downregulates tissue factor expression and activity by modulating growth factor receptor-mediated induction of nuclear factor κB (2013)
Thesis
Fountain, D. L. (2013). Low molecular weight heparin downregulates tissue factor expression and activity by modulating growth factor receptor-mediated induction of nuclear factor κB. (Thesis). University of Hull. Retrieved from https://hull-repository.worktribe.com/output/4215936

The association between thrombosis and cancer has long been established. Tissue factor (TF), the initiator of the extrinsic pathway in the blood coagulation cascade, has been implicated as one of the most important physiological factors causing such... Read More about Low molecular weight heparin downregulates tissue factor expression and activity by modulating growth factor receptor-mediated induction of nuclear factor κB.

Enhanced binding of tissue factor-microparticles to collagen-IV and fibronectin leads to increased tissue factor activity in vitro (2012)
Journal Article
Ettelaie, C., Collier, M. E. W., Mei, M. P., Xiao, Y. P., & Maraveyas, A. (2013). Enhanced binding of tissue factor-microparticles to collagen-IV and fibronectin leads to increased tissue factor activity in vitro. Thrombosis and haemostasis, 109(1), 61-71. https://doi.org/10.1160/TH12-05-0279

The role of tissue factor (TF)-containing microparticles in clot propagation has been established, but the ability of circulating microparticles to initiate coagulation has been disputed. However, TF-bearing microparticles, particularly endothelial-m... Read More about Enhanced binding of tissue factor-microparticles to collagen-IV and fibronectin leads to increased tissue factor activity in vitro.

Low molecular weight heparin downregulates tissue factor expression and activity by modulating growth factor receptor-mediated induction of nuclear factor-κB (2011)
Journal Article
Ettelaie, C., Fountain, D., Collier, M. E. W., ElKeeb, A. M., Xiao, Y. P., & Maraveyas, A. (2011). Low molecular weight heparin downregulates tissue factor expression and activity by modulating growth factor receptor-mediated induction of nuclear factor-κB. Biochimica et Biophysica Acta (BBA): Molecular Basis of Disease, 1812(12), 1591-1600. https://doi.org/10.1016/j.bbadis.2011.09.007

Treatment of cancer patients with low molecular weight heparin (LMWH) appears to have beneficial effects. In this study, the influence of low molecular weight heparin (LMWH) on tissue factor (TF) expression and activity in five cell lines from variou... Read More about Low molecular weight heparin downregulates tissue factor expression and activity by modulating growth factor receptor-mediated induction of nuclear factor-κB.