Analysis of the potential of cancer cell lines to release tissue factor-containing microvesicles: correlation with tissue factor and PAR2 expression

Ettelaie, Camille; Collier, Mary E. W.; Featherby, Sophie; Benelhaj, Naima E.; Greenman, John; Maraveyas, Anthony

Abstract

Background
Despite the association of cancer-derived circulating tissue factor (TF)-containing microvesicles and hypercoagulable state, correlations with the incidence of thrombosis remain unclear.

Methods
In this study the upregulation of TF release upon activation of various cancer cell lines, and the correlation with TF and PAR2 expression and/or activity was examined. Microvesicle release was induced by PAR2 activation in seventeen cell lines and released microvesicle density, microvesicle-associated TF activity, and phoshpatidylserine-mediated activity were measured. The time-course for TF release was monitored over 90 min in each cell line. In addition, TF mRNA expression, cellular TF protein and cell-surface TF activities were quantified. Moreover, the relative expression of PAR2 mRNA and cellular protein were analysed. Any correlations between the above parameters were examined by determining the Pearson’s correlation coefficients.

Results
TF release as microvesicles peaked between 30–60 min post-activation in the majority of cell lines tested. The magnitude of the maximal TF release positively correlated with TF mRNA (c = 0.717; p 

Citation

Ettelaie, C., Collier, M. E. W., Featherby, S., Benelhaj, N. E., Greenman, J., & Maraveyas, A. (2016). Analysis of the potential of cancer cell lines to release tissue factor-containing microvesicles: correlation with tissue factor and PAR2 expression. Thrombosis journal, 14(2), Article ARTN 2. https://doi.org/10.1186/s12959-016-0075-3

• Journal Article Type: Article
• Acceptance Date: Jan 10, 2016
• Online Publication Date: Jan 19, 2016
• Publication Date: Jan 19, 2016
• Deposit Date: Feb 16, 2016
• Publicly Available Date: Feb 16, 2016
• Journal: Thrombosis journal
• Print ISSN: 1477-9560
• Publisher: Springer Verlag
• Peer Reviewed: Peer Reviewed
• Volume: 14
• Issue: 2
• Article Number: ARTN 2
• DOI: https://doi.org/10.1186/s12959-016-0075-3
• Keywords: Tissue factor, Microvesicles, PAR 2, Blood coagulation, Cell line
• Public URL: https://hull-repository.worktribe.com/output/471712
• Publisher URL: http://thrombosisjournal.biomedcentral.com/articles/10.1186/s12959-016-0075-3
• Additional Information: Copy of article first published in: Thrombosis journal, 2016, v.14, issue 2.
• Contract Date: Feb 16, 2016