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Analysis of the potential of cancer cell lines to release tissue factor-containing microvesicles: correlation with tissue factor and PAR2 expression

Ettelaie, Camille; Collier, Mary E. W.; Featherby, Sophie; Benelhaj, Naima E.; Greenman, John; Maraveyas, Anthony


Camille Ettelaie

Mary E. W. Collier

Sophie Featherby

Naima E. Benelhaj

Anthony Maraveyas


Despite the association of cancer-derived circulating tissue factor (TF)-containing microvesicles and hypercoagulable state, correlations with the incidence of thrombosis remain unclear.

In this study the upregulation of TF release upon activation of various cancer cell lines, and the correlation with TF and PAR2 expression and/or activity was examined. Microvesicle release was induced by PAR2 activation in seventeen cell lines and released microvesicle density, microvesicle-associated TF activity, and phoshpatidylserine-mediated activity were measured. The time-course for TF release was monitored over 90 min in each cell line. In addition, TF mRNA expression, cellular TF protein and cell-surface TF activities were quantified. Moreover, the relative expression of PAR2 mRNA and cellular protein were analysed. Any correlations between the above parameters were examined by determining the Pearson’s correlation coefficients.

TF release as microvesicles peaked between 30–60 min post-activation in the majority of cell lines tested. The magnitude of the maximal TF release positively correlated with TF mRNA (c = 0.717; p 

Journal Article Type Article
Publication Date Jan 19, 2016
Journal Thrombosis journal
Print ISSN 1477-9560
Electronic ISSN 1477-9560
Publisher Springer Verlag
Peer Reviewed Peer Reviewed
Volume 14
Issue 2
Article Number ARTN 2
Keywords Tissue factor, Microvesicles, PAR 2, Blood coagulation, Cell line
Publisher URL
Copyright Statement © Ettelaie et al. 2016
Additional Information Copy of article first published in: Thrombosis journal, 2016, v.14, issue 2.


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