An internal polyadenylation signal substantially increases expression levels of lentivirus-delivered transgenes but has the potential to reduce viral titer in a promoter-dependent manner

Hager, Stefanie; Frame, Fiona M.; Collins, Anne T.; Burns, Julie E.; Maitland, Norman J.

doi:10.1089/hum.2007.165

All Outputs (2)

Prostate cancer stem cells (2008)
Journal Article
Lang, S., Frame, F., & Collins, A. (2009). Prostate cancer stem cells. Journal of Pathology, 217(2), 299-306. https://doi.org/10.1002/path.2478

Despite the discovery over 60 years ago by Huggins and Hodges that prostate cancers respond to androgen deprivation therapy, hormone‐refractory prostate cancer remains a major clinical challenge. There is now mounting evidence that solid tumours orig... Read More about Prostate cancer stem cells.

An internal polyadenylation signal substantially increases expression levels of lentivirus-delivered transgenes but has the potential to reduce viral titer in a promoter-dependent manner (2008)
Journal Article
Hager, S., Frame, F. M., Collins, A. T., Burns, J. E., & Maitland, N. J. (2008). An internal polyadenylation signal substantially increases expression levels of lentivirus-delivered transgenes but has the potential to reduce viral titer in a promoter-dependent manner. Human Gene Therapy, 19(8), 840-850. https://doi.org/10.1089/hum.2007.165

In lentiviral gene delivery systems, transgene expression cassettes are commonly cloned without a polyadenylation signal to prevent disruption of full-length lentiviral genomes on mRNA maturation in producer cells. The lack of the polyadenylation sig... Read More about An internal polyadenylation signal substantially increases expression levels of lentivirus-delivered transgenes but has the potential to reduce viral titer in a promoter-dependent manner.