All Outputs (8)

Optimising species detection probability and sampling effort in lake fish eDNA surveys (2024)
Journal Article
Sellers, G. S., Jerde, C. L., Harper, L. R., Benucci, M., Muri, C. D., Li, J., Peirson, G., Walsh, K., Hatton-Ellis, T., Duncan, W., Duguid, A., Ottewell, D., Willby, N., Law, A., Bean, C. W., Winfield, I. J., Read, D. S., Handley, L. L., & Hänfling, B. (2024). Optimising species detection probability and sampling effort in lake fish eDNA surveys. Metabarcoding and Metagenomics, 8, 121–143. https://doi.org/10.3897/mbmg.8.104655

Environmental DNA (eDNA) metabarcoding is transforming biodiversity monitoring in aquatic environments. Such an approach has been developed and deployed for monitoring lake fish communities in Great Britain, where the method has repeatedly shown a co... Read More about Optimising species detection probability and sampling effort in lake fish eDNA surveys.

Environmental DNA metabarcoding for fish diversity assessment in a macrotidal estuary: A comparison with established fish survey methods (2023)
Journal Article
Gibson, T. I., Carvalho, G., Ellison, A., Gargiulo, E., Hatton-Ellis, T., Lawson-Handley, L., …Creer, S. (2023). Environmental DNA metabarcoding for fish diversity assessment in a macrotidal estuary: A comparison with established fish survey methods. Estuarine, coastal and shelf science, 294, Article 108522. https://doi.org/10.1016/j.ecss.2023.108522

Fishes are a dominant component of the macrofauna in estuaries and are important for assessing the health of these threatened ecosystems. Several studies have applied environmental DNA (eDNA) metabarcoding to assess the biodiversity of fishes in estu... Read More about Environmental DNA metabarcoding for fish diversity assessment in a macrotidal estuary: A comparison with established fish survey methods.

Integrating environmental DNA monitoring to inform eel (Anguilla anguilla) status in freshwaters at their easternmost range—A case study in Cyprus (2023)
Journal Article
Griffiths, N. P., Wright, R. M., Hänfling, B., Bolland, J. D., Drakou, K., Sellers, G. S., …Vasquez, M. I. (2023). Integrating environmental DNA monitoring to inform eel (Anguilla anguilla) status in freshwaters at their easternmost range—A case study in Cyprus. Ecology and Evolution, 13(2), Article e9800. https://doi.org/10.1002/ece3.9800

Despite significant population declines and targeted European Union regulations aimed at Anguilla anguilla conservation, little attention has been given to their status at their easternmost range. This study applies wide-scale integrated monitoring t... Read More about Integrating environmental DNA monitoring to inform eel (Anguilla anguilla) status in freshwaters at their easternmost range—A case study in Cyprus.

DNA metabarcoding reveals the dietary profiles of a benthic marine crustacean, Nephrops norvegicus (2023)
Journal Article
Shum, P., Wäge-Recchioni, J., Sellers, G. S., Johnson, M. L., & Joyce, D. A. (2023). DNA metabarcoding reveals the dietary profiles of a benthic marine crustacean, Nephrops norvegicus. PLoS ONE, 18(11 November), Article e0289221. https://doi.org/10.1371/journal.pone.0289221

Norwegian lobster, Nephrops norvegicus, are a generalist scavenger and predator capable of short foraging excursions but can also suspension feed. Existing knowledge about their diet relies on a combination of methods including morphology-based stoma... Read More about DNA metabarcoding reveals the dietary profiles of a benthic marine crustacean, Nephrops norvegicus.

Identification of individual root-knot nematodes using low coverage long-read sequencing (2021)
Journal Article
Sellers, G. S., Jeffares, D. C., Lawson, B., Prior, T., & Lunt, D. H. (2021). Identification of individual root-knot nematodes using low coverage long-read sequencing. PLoS ONE, 16(12), Article e0253248. https://doi.org/10.1371/journal.pone.0253248

Root-knot nematodes (RKN; genus Meloidogyne) are polyphagous plant pathogens of great economic importance to agriculturalists globally. These species are small, diverse, and can be challenging for accurate taxonomic identification. Many of the most i... Read More about Identification of individual root-knot nematodes using low coverage long-read sequencing.

Simple, sensitive and species-specific assays for detecting quagga and zebra mussels (Dreissena rostriformis bugensis and D. polymorpha) using environmental DNA (2020)
Journal Article
Blackman, R. C., Benucci, M., Donnelly, R., Hänfling, B., Harper, L. R., Sellers, G. S., & Lawson-Handley, L. (2020). Simple, sensitive and species-specific assays for detecting quagga and zebra mussels (Dreissena rostriformis bugensis and D. polymorpha) using environmental DNA. Management of Biological Invasions, 11(2), 218-236. https://doi.org/10.3391/mbi.2020.11.2.04

Early detection is paramount for attempts to remove invasive non-native species (INNS). Traditional methods rely on physical sampling and morphological identification, which can be problematic when species are in low densities and/or are cryptic. The... Read More about Simple, sensitive and species-specific assays for detecting quagga and zebra mussels (Dreissena rostriformis bugensis and D. polymorpha) using environmental DNA.

Mu-DNA: a modular universal DNA extraction method adaptable for a wide range of sample types (2018)
Journal Article
Sellers, G. S., Di Muri, C., Gómez, A., & Hänfling, B. (2018). Mu-DNA: a modular universal DNA extraction method adaptable for a wide range of sample types. Metabarcoding and Metagenomics, 2, e24556. https://doi.org/10.3897/mbmg.2.24556

Efficient DNA extraction is fundamental to molecular studies. However, commercial kits are expensive when a large number of samples need to be processed. Here we present a simple, modular and adaptable DNA extraction ‘toolkit’ for the isolation of hi... Read More about Mu-DNA: a modular universal DNA extraction method adaptable for a wide range of sample types.

A new molecular diagnostic tool for surveying and monitoring Triops cancriformis populations (2017)
Journal Article
Sellers, G. S., Griffin, L. R., Hänfling, B., & Gómez, A. (2017). A new molecular diagnostic tool for surveying and monitoring Triops cancriformis populations. PeerJ, 2017(5), Article e3228. https://doi.org/10.7717/peerj.3228

© 2017 Sellers et al. The tadpole shrimp, Triops cancriformis, is a freshwater crustacean listed as endangered in the UK and Europe living in ephemeral pools. Populations are threatened by habitat destruction due to land development for agriculture a... Read More about A new molecular diagnostic tool for surveying and monitoring Triops cancriformis populations.