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Diminution in sperm quantity and quality in mouse models of Duchenne Muscular Dystrophy induced by a myostatin-based muscle growth-promoting intervention (2020)
Journal Article
Vaughan, D., Kretz, O., Alqallaf, A., Mitchell, R., von der Heide, J., Vaiyapuri, S., …Patel, K. (in press). Diminution in sperm quantity and quality in mouse models of Duchenne Muscular Dystrophy induced by a myostatin-based muscle growth-promoting intervention. European Journal of Translational Myology, 30(2), 276-285. https://doi.org/10.4081/ejtm.0.8904

Duchenne Muscular Dystrophy is a devastating disease caused by the absence of a functional rod-shaped cytoplasmic protein called dystrophin. Several avenues are being developed aimed to restore dystrophin expression in boys affected by this X-linked... Read More about Diminution in sperm quantity and quality in mouse models of Duchenne Muscular Dystrophy induced by a myostatin-based muscle growth-promoting intervention.

Metabolic profile of in-vitro derived human embryos is not affected by the mode of fertilization (2020)
Journal Article
Sturmey, R. G., & Leary, C. (2020). Metabolic profile of in-vitro derived human embryos is not affected by the mode of fertilization. Molecular Human Reproduction, 26(4), 277-287. https://doi.org/10.1093/molehr/gaaa015

The pattern of metabolism by early embryos in vitro has been linked to a range of phenotypes, including viability. However, the extent to which metabolic function of embryos is modified by specific methods used during ART has yet to be fully describ... Read More about Metabolic profile of in-vitro derived human embryos is not affected by the mode of fertilization.

Spatial and Pregnancy-Related Changes in the Protein, Amino Acid, and Carbohydrate Composition of Bovine Oviduct Fluid (2020)
Journal Article
Rodríguez-Alonso, B., Maillo, V., Acuña, O. S., López-Úbeda, R., Torrecillas, A., Simintiras, C. A., …Rizos, D. (2020). Spatial and Pregnancy-Related Changes in the Protein, Amino Acid, and Carbohydrate Composition of Bovine Oviduct Fluid. International Journal of Molecular Sciences, 21(5), https://doi.org/10.3390/ijms21051681

Knowledge of how the biochemical composition of the bovine oviduct is altered due to the oviduct anatomy or the presence of an embryo is lacking. Thus, the aim of this study was to assess the effect of (І) oviduct anatomy and (ІІ) embryo presence on... Read More about Spatial and Pregnancy-Related Changes in the Protein, Amino Acid, and Carbohydrate Composition of Bovine Oviduct Fluid.

Good practice recommendations for the use of time-lapse technology† (2020)
Journal Article
Apter, S., Ebner, T., Freour, T., Guns, Y., Kovacic, B., Le Clef, N., …ESHRE Working group on Time-lapse technology, . (2020). Good practice recommendations for the use of time-lapse technology†. Human Reproduction Open, 2020(2), https://doi.org/10.1093/hropen/hoaa008

STUDY QUESTION: What recommendations can be provided on the approach to and use of time-lapse technology (TLT) in an IVF laboratory? SUMMARY ANSWER: The present ESHRE document provides 11 recommendations on how to introduce TLT in the IVF laboratory... Read More about Good practice recommendations for the use of time-lapse technology†.

Removal of Human Leukemic Cells from Peripheral Blood Mononuclear Cells by Cell Recognition Chromatography with Size Matched Particle Imprints (2020)
Journal Article
Chester, R., Das, A. A. K., Medlock, J., Nees, D., Allsup, D. J., Madden, L. A., & Paunov, V. N. (2020). Removal of Human Leukemic Cells from Peripheral Blood Mononuclear Cells by Cell Recognition Chromatography with Size Matched Particle Imprints. ACS Applied Bio Materials, 3(2), 789-800. https://doi.org/10.1021/acsabm.9b00770

We report a cell recognition chromatography approach for blood cancer cell separation from healthy peripheral blood mononuclear cells (PBMCs) based on sizematched functionalized particle imprints. Negative imprints were prepared from layers of 15 μm... Read More about Removal of Human Leukemic Cells from Peripheral Blood Mononuclear Cells by Cell Recognition Chromatography with Size Matched Particle Imprints.

Biochemical and immunocytochemical characterization of coronins in platelets (2019)
Journal Article
Riley, D. R., Khalil, J. S., Naseem, K. M., & Rivero, F. (in press). Biochemical and immunocytochemical characterization of coronins in platelets. Platelets, 1-12. https://doi.org/10.1080/09537104.2019.1696457

Results Platelets express at least four coronins Proteomics and transcriptomics studies indicate that both human and mouse platelets express Coro1, 2, 3 and 7, while other coronins are practically undetectable (Supplemental Table 1 and 2). To demonst... Read More about Biochemical and immunocytochemical characterization of coronins in platelets.

Application of extracellular flux analysis for determining mitochondrial function in mammalian oocytes and early embryos (2019)
Journal Article
Muller, B., Lewis, N., Adeniyi, T., Leese, H. J., Brison, D. R., & Sturmey, R. G. (2019). Application of extracellular flux analysis for determining mitochondrial function in mammalian oocytes and early embryos. Scientific reports, 9, https://doi.org/10.1038/s41598-019-53066-9

Mitochondria provide the major source of ATP for mammalian oocyte maturation and early embryo development. Oxygen Consumption Rate (OCR) is an established measure of mitochondrial function. OCR by mammalian oocytes and embryos has generally been rest... Read More about Application of extracellular flux analysis for determining mitochondrial function in mammalian oocytes and early embryos.

Enhanced Clearing of Wound-Related Pathogenic Bacterial Biofilms Using Protease-Functionalized Antibiotic Nanocarriers (2019)
Journal Article
Weldrick, P. J., Hardman, M. J., & Paunov, V. N. (2019). Enhanced Clearing of Wound-Related Pathogenic Bacterial Biofilms Using Protease-Functionalized Antibiotic Nanocarriers. ACS applied materials & interfaces, 11(47), 43902-43919. https://doi.org/10.1021/acsami.9b16119

© 2019 American Chemical Society. Biofilms are prevalent in chronic wounds and once formed are very hard to remove, which is associated with poor outcomes and high mortality rates. Biofilms are comprised of surface-attached bacteria embedded in an ex... Read More about Enhanced Clearing of Wound-Related Pathogenic Bacterial Biofilms Using Protease-Functionalized Antibiotic Nanocarriers.

Optimising platelet secretomes to deliver robust tissue‐specific regeneration (2019)
Journal Article
Scully, D., Sfyri, P., Wilkinson, H. N., Acebes‐Huerta, A., Verpoorten, S., Muñoz‐Turrillas, M. C., …Matsakas, A. (in press). Optimising platelet secretomes to deliver robust tissue‐specific regeneration. Journal of tissue engineering and regenerative medicine, https://doi.org/10.1002/term.2965

Promoting cell proliferation is the cornerstone of most tissue regeneration therapies. As platelet‐based applications promote cell division and can be customised for tissue‐specific efficacy, this makes them strong candidates for developing novel r... Read More about Optimising platelet secretomes to deliver robust tissue‐specific regeneration.

Loss of CD36 protects against diet‐induced obesity but results in impaired muscle stem cell function, delayed muscle regeneration and hepatic steatosis (2019)
Journal Article
Verpoorten, S., Sfyri, P., Scully, D., Mitchell, R., Tzimou, A., Mougios, V., …Matsakas, A. (in press). Loss of CD36 protects against diet‐induced obesity but results in impaired muscle stem cell function, delayed muscle regeneration and hepatic steatosis. Acta Physiologica, https://doi.org/10.1111/apha.13395

Aim: The prevalence of obesity is a major risk factor for cardiovascular and metabolic diseases including impaired skeletal muscle regeneration. Since skeletal muscle regenerative capacity is regulated by satellite cells, we aimed to investigate whet... Read More about Loss of CD36 protects against diet‐induced obesity but results in impaired muscle stem cell function, delayed muscle regeneration and hepatic steatosis.

Tissue iron promotes wound repair via M2 macrophage polarisation and the chemokines CCL17 and CCL22 (2019)
Journal Article
Wilkinson, H. N., Roberts, E. R., Stafford, A. R., Banyard, K. L., Matteucci, P., Mace, K. A., & Hardman, M. J. (2019). Tissue iron promotes wound repair via M2 macrophage polarisation and the chemokines CCL17 and CCL22. American Journal of Pathology, https://doi.org/10.1016/j.ajpath.2019.07.015

Macrophages are important for effective iron recycling and erythropoiesis, but they also play a crucial role in wound healing, orchestrating tissue repair. Recently, we demonstrated a significant accumulation of iron in healing wounds and a requireme... Read More about Tissue iron promotes wound repair via M2 macrophage polarisation and the chemokines CCL17 and CCL22.

T-type Ca 2+ channel blocker mibefradil blocks ORAI channels via acting on extracellular surface (2019)
Journal Article
Li, P., Rubaiy, H. N., Chen, G., Hallett, T., Zaibi, N., Zeng, B., …Xu, S. (in press). T-type Ca 2+ channel blocker mibefradil blocks ORAI channels via acting on extracellular surface. British Journal of Pharmacology, https://doi.org/10.1111/bph.14788

Background and purpose Mibefradil (Mib), a T‐type Ca2+ channel blocker, has been investigated for treating solid tumours. However, its underlying mechanisms are still unclear. Here we aimed to investigate the pharmacological aspect of Mib on ORAI st... Read More about T-type Ca 2+ channel blocker mibefradil blocks ORAI channels via acting on extracellular surface.

Germline selection shapes human mitochondrial DNA diversity (2019)
Journal Article
Wei, W., Tuna, S., Keogh, M. J., Smith, K. R., Aitman, T. J., Beales, P. L., …Chinnery, P. F. (2019). Germline selection shapes human mitochondrial DNA diversity. Science, 364(6442), https://doi.org/10.1126/science.aau6520

INTRODUCTION Only 2.4% of the 16.5-kb mitochondrial DNA (mtDNA) genome shows homoplasmic variation at >1% frequency in humans. Migration patterns have contributed to geographic differences in the frequency of common genetic variants, but population... Read More about Germline selection shapes human mitochondrial DNA diversity.

Breathing new life into old antibiotics: Overcoming antibacterial resistance by antibiotic-loaded nanogel carriers with cationic surface functionality (2019)
Journal Article
Weldrick, P. J., Iveson, S., Hardman, M. J., & Paunov, V. N. (2019). Breathing new life into old antibiotics: Overcoming antibacterial resistance by antibiotic-loaded nanogel carriers with cationic surface functionality. Nanoscale, 11(21), 10472-10485. https://doi.org/10.1039/c8nr10022e

Multidrug-resistant pathogens are prevalent in chronic wounds. There is an urgent need to develop novel antimicrobials and formulation strategies that can overcome antibiotic resistance and provide a safe alternative to traditional antibiotics. This... Read More about Breathing new life into old antibiotics: Overcoming antibacterial resistance by antibiotic-loaded nanogel carriers with cationic surface functionality.

Bioimprint aided cell recognition and depletion of human leukemic HL60 cells from peripheral blood (2019)
Journal Article
Das, A. A., Medlock, J., Liang, H., Nees, D., Allsup, D. J., Madden, L. A., & Paunov, V. N. (2019). Bioimprint aided cell recognition and depletion of human leukemic HL60 cells from peripheral blood. Journal of materials chemistry B, Materials for biology and medicine /, 7(22), 3497-3504. https://doi.org/10.1039/c9tb00679f

We report a large scale preparation of bioimprints of layers of cultured human leukemic HL60 cells which can perform cell shape and size recognition from a mixture with peripheral blood mononuclear cells (PBMCs). We demonstrate that the bioimprint-ce... Read More about Bioimprint aided cell recognition and depletion of human leukemic HL60 cells from peripheral blood.

Application of extracellular flux analysis for determining mitochondrial function in mammalian oocytes and early embryos (2019)
Working Paper
Muller, B., Lewis, N., Adeniyi, T., Leese, H. J., Brison, D., & Sturmey, R. G. Application of extracellular flux analysis for determining mitochondrial function in mammalian oocytes and early embryos

Background Mitochondria provide the major source of ATP for mammalian oocyte maturation and early embryo development. Oxygen Consumption Rate (OCR) is an established measure of mitochondrial function. OCR by mammalian oocytes and embryos has generall... Read More about Application of extracellular flux analysis for determining mitochondrial function in mammalian oocytes and early embryos.

Gene expression and metabolic response of bovine oviduct epithelial cells to the early embryo (2019)
Journal Article
Hamdi, M., Sánchez-Calabuig, M. J., Rodríguez-Alonso, B., Bagés-Arnal, S., Roussi, K., Sturmey, R., …Rizos, D. (2019). Gene expression and metabolic response of bovine oviduct epithelial cells to the early embryo. Reproduction, 158(1), 85-94. https://doi.org/10.1530/REP-18-0561

During its journey through the oviduct, the bovine embryo may induce transcriptomic and metabolic responses, via direct or indirect contact, from bovine oviduct epithelial cells (BOEC). An in vitro model using polyester mesh was established, allowing... Read More about Gene expression and metabolic response of bovine oviduct epithelial cells to the early embryo.

Compression of morbidity in a progeroid mouse model through the attenuation of myostatin/activin signalling (2019)
Journal Article
Alyodawi, K., Vermeij, W., Omairi, S., Kretz, O., Solagna, F., Joch, B., …Hoeijmakers, J. (2019). Compression of morbidity in a progeroid mouse model through the attenuation of myostatin/activin signalling. Journal of Cachexia, Sarcopenia and Muscle, 10(3), 662-686. https://doi.org/10.1002/jcsm.12404

Background One of the principles underpinning our understanding of ageing is that DNA damage induces a stress response that shifts cellular resources from growth towards maintenance. A contrasting and seemingly irreconcilable view is that prompting... Read More about Compression of morbidity in a progeroid mouse model through the attenuation of myostatin/activin signalling.


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