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Application of extracellular flux analysis for determining mitochondrial function in mammalian oocytes and early embryos (2019)
Working Paper
Muller, B., Lewis, N., Adeniyi, T., Leese, H. J., Brison, D., & Sturmey, R. G. Application of extracellular flux analysis for determining mitochondrial function in mammalian oocytes and early embryos

Background Mitochondria provide the major source of ATP for mammalian oocyte maturation and early embryo development. Oxygen Consumption Rate (OCR) is an established measure of mitochondrial function. OCR by mammalian oocytes and embryos has generall... Read More

Bioimprint aided cell recognition and depletion of human leukemic HL60 cells from peripheral blood (2019)
Journal Article
Das, A. A., Medlock, J., Liang, H., Nees, D., Allsup, D. J., Madden, L. A., & Paunov, V. N. (in press). Bioimprint aided cell recognition and depletion of human leukemic HL60 cells from peripheral blood. Journal of materials chemistry B, Materials for biology and medicine /, https://doi.org/10.1039/c9tb00679f

We report a large scale preparation of bioimprints of layers of cultured human leukemic HL60 cells which can perform cell shape and size recognition from a mixture with peripheral blood mononuclear cells (PBMCs). We demonstrate that the bioimprint-ce... Read More

Gene expression and metabolic response of bovine oviduct epithelial cells to the early embryo (2019)
Journal Article
Hamdi, M., Sánchez-Calabuig, M. J., Rodríguez-Alonso, B., Bagés-Arnal, S., Roussi, K., Sturmey, R., …Rizos, D. (in press). Gene expression and metabolic response of bovine oviduct epithelial cells to the early embryo. Reproduction, https://doi.org/10.1530/REP-18-0561

During its journey through the oviduct, the bovine embryo may induce transcriptomic and metabolic responses, via direct or indirect contact, from bovine oviduct epithelial cells (BOEC). An in vitro model using polyester mesh was established, allowing... Read More

The enigmatic morula: mechanisms of development, cell fate determination, self-correction and implications for ART (2019)
Journal Article
Coticchio, G., Lagalla, C., Sturmey, R., Pennetta, F., & Borini, A. (in press). The enigmatic morula: mechanisms of development, cell fate determination, self-correction and implications for ART. Human Reproduction Update, https://doi.org/10.1093/humupd/dmz008

Background: Assisted reproduction technology offers the opportunity to observe the very early stages of human development. However, due to practical constraints, for decades morphological examination of embryo development has been undertaken at a few... Read More

N-cinnamoylanthranilates as human TRPA1 modulators: Structure-activity relationships and channel binding sites (2019)
Journal Article
Chandrabalan, A., McPhillie, M. J., Morice, A. H., Boa, A. N., & Sadofsky, L. R. (2019). N-cinnamoylanthranilates as human TRPA1 modulators: Structure-activity relationships and channel binding sites. European Journal of Medicinal Chemistry, doi:10.1016/j.ejmech.2019.02.074

The transient receptor potential ankyrin 1 (TRPA1) channel is a non-selective cation channel, which detects noxious stimuli leading to pain, itch and cough. However, the mechanism(s) of channel modulation by many of the known, non-reactive modulators... Read More

Regulation of the dystrophin-associated glycoprotein complex composition by the metabolic properties of muscle fibres (2019)
Journal Article
Omairi, S., Hau, K., Collins-Hooper, H., Scott, C., Vaiyapuri, S., Torelli, S., …Patel, K. (2019). Regulation of the dystrophin-associated glycoprotein complex composition by the metabolic properties of muscle fibres. Scientific reports, 9(1), doi:10.1038/s41598-019-39532-4

The dystrophin-glycoprotein complex (DGC) links the muscle cytoskeleton to the extracellular matrix and is responsible for force transduction and protects the muscle fibres from contraction induced damage. Mutations in components of the DGC are respo... Read More

Survey of registered nurses’ selection of compression systems for the treatment of venous leg ulcers in the UK (2019)
Journal Article
Oates, A., & Adderley, U. (2019). Survey of registered nurses’ selection of compression systems for the treatment of venous leg ulcers in the UK. Journal of tissue viability, doi:10.1016/j.jtv.2019.02.004

Background: Venous leg ulceration is common in older adults in the United Kingdom. The gold-standard treatment is compression therapy. There are several compression bandage and hosiery systems that can be prescribed or purchased, but it was unclear w... Read More

Alterations in platelet alpha granule secretion and adhesion on collagen under flow in mice lacking the atypical Rho GTPase RhoBTB3 (2019)
Journal Article
Berger, M., Riley, D. R. J., Lutz, J., Khalil, J. S., Aburima, A., Naseem, K. M., & Rivero, F. (2019). Alterations in platelet alpha granule secretion and adhesion on collagen under flow in mice lacking the atypical Rho GTPase RhoBTB3. Cells, 8(2), 149. doi:10.3390/cells8020149

Typical Rho GTPases, like Rac1, Cdc42 and RhoA, act as molecular switches regulating various aspects of platelet cytoskeleton reorganization. The loss of these enzymes results in reduced platelet functionality. Atypical Rho GTPases of the RhoBTB su... Read More

Elevated local senescence in diabetic wound healing is linked to pathological repair via CXCR2. (2019)
Journal Article
Wilkinson, H. N., Clowes, C., Banyard, K. L., Matteuci, P., Mace, K., & Hardman, M. J. (in press). Elevated local senescence in diabetic wound healing is linked to pathological repair via CXCR2. Journal of Investigative Dermatology, doi:10.1016/j.jid.2019.01.005

Cellular senescence can be broadly defined as a stable, yet essentially irreversible, loss of proliferative capacity. Historically, senescence is described as a negative outcome of advanced cellular age. It is now clear, however, that senescence repr... Read More

Therapeutic targeting of the proinflammatory IL-6-JAK/STAT signalling pathways responsible for vascular restenosis in Type 2 diabetes mellitus (2019)
Journal Article
Florah, T., Moshapa, , Riches-Suman, K., Palmer, T. M., & Palmer, T. M. (2019). Therapeutic targeting of the proinflammatory IL-6-JAK/STAT signalling pathways responsible for vascular restenosis in Type 2 diabetes mellitus. Cardiology Research and Practice, 2019, doi:10.1155/2019/9846312

Type 2 diabetes mellitus (T2DM) is increasing worldwide, and it is associated with increased risk of coronary artery disease (CAD). For T2DM patients, the main surgical intervention for CAD is autologous saphenous vein grafting. However, T2DM patient... Read More

A769662 inhibits insulin-stimulated akt activation in human macrovascular endothelial cells independent of AMP-activated protein kinase (2018)
Journal Article
Strembitska, A., Mancini, S. J., Gamwell, J. M., Palmer, T. M., Baillie, G. S., & Salt, I. P. (2018). A769662 inhibits insulin-stimulated akt activation in human macrovascular endothelial cells independent of AMP-activated protein kinase. International Journal of Molecular Sciences, 19(12), 3886. doi:10.3390/ijms19123886

Protein kinase B (Akt) is a key enzyme in the insulin signalling cascade, required for insulin-stimulated NO production in endothelial cells (ECs). Previous studies have suggested that AMP-activated protein kinase (AMPK) activation stimulates NO synt... Read More

Platelet releasate promotes skeletal myogenesis by increasing muscle stem cell commitment to differentiation and accelerates muscle regeneration following acute injury (2018)
Journal Article
Scully, D., Sfyri, P., Verpoorten, S., Papadopoulos, P., Muñoz-Turrillas, M. C., Mitchell, R., …Matsakas, A. (2019). Platelet releasate promotes skeletal myogenesis by increasing muscle stem cell commitment to differentiation and accelerates muscle regeneration following acute injury. Acta Physiologica, 225(3), doi:10.1111/apha.13207

Aim The use of platelets as biomaterials has gained intense research interest. However, the mechanisms regarding platelet‐mediated skeletal myogenesis remain to be established. The aim of this study was to determine the role of platelet releasate in... Read More

Attenuation of oxidative stress-induced lesions in skeletal muscle in a mouse model of obesity-independent hyperlipidaemia and atherosclerosis through the inhibition of Nox2 activity (2018)
Journal Article
Sfyri, P. P., Yuldasheva, N. Y., Tzimou, A., Giallourou, N., Crispi, V., Aburima, A., …Matsakas, A. (2018). Attenuation of oxidative stress-induced lesions in skeletal muscle in a mouse model of obesity-independent hyperlipidaemia and atherosclerosis through the inhibition of Nox2 activity. Free radical biology & medicine, 129, 504-519. doi:10.1016/j.freeradbiomed.2018.10.422

Obesity leading to hyperlipidaemia and atherosclerosis is recognised to induce morphological and metabolic changes in many tissues. However, hyperlipidaemia can occur in the absence of obesity. The impact of the latter scenario on skeletal muscle and... Read More

A comprehensive targeted next-generation sequencing panel for genetic diagnosis of patients with suspected inherited thrombocytopenia (2018)
Journal Article
Johnson, B., Doak, R., Allsup, D., Astwood, E., Evans, G., Grimley, C., …the UK GAPP Study Group, . (2018). A comprehensive targeted next-generation sequencing panel for genetic diagnosis of patients with suspected inherited thrombocytopenia. Research and Practice in Thrombosis and Haemostasis, 2(4), 640-652. doi:10.1002/rth2.12151

Background Inherited thrombocytopenias (ITs) are a heterogeneous group of disorders characterized by low platelet counts and often disproportionate bleeding with over 30 genes currently implicated. Previously the UK‐GAPP study using whole exome sequ... Read More

Glucose metabolism and metabolic flexibility in blood platelets (2018)
Journal Article
Aibibula, M., Naseem, K. M., & Sturmey, R. (2018). Glucose metabolism and metabolic flexibility in blood platelets. Journal of thrombosis and haemostasis : JTH, 16(11), 2300-2314. doi:10.1111/jth.14274

The metabolic integration processes required for platelet activation are unclear. The metabolic plasticity of human platelets were investigated. Activated platelets exhibit a glycolytic phenotype while preserving mitochondrial function. Platelets can... Read More

Attenuation of autophagy impacts on muscle fibre development, starvation induced stress and fibre regeneration following acute injury (2018)
Journal Article
Paolini, A., Omairi, S., Mitchell, R., Vaughan, D., Matsakas, A., Vaiyapuri, S., …Patel, K. (in press). Attenuation of autophagy impacts on muscle fibre development, starvation induced stress and fibre regeneration following acute injury. Scientific reports, 8(1), doi:10.1038/s41598-018-27429-7

Autophagy has been implicated as a major factor in the development of a number of diseases of skeletal muscle. However, its role in skeletal muscle homeostasis is still evolving. We examined skeletal muscle architecture in a mouse model, Atg16L1, whe... Read More

Effect of metabolic status on conceptus–maternal interactions on day 19 in dairy cattle: II. Effects on the endometrial transcriptome (2017)
Journal Article
Bauersachs, S., Simintiras, C. A., Sturmey, R. G., Krebs, S., Bick, J., Blum, H., …Forde, N. (2017). Effect of metabolic status on conceptus–maternal interactions on day 19 in dairy cattle: II. Effects on the endometrial transcriptome. Biology of Reproduction, 97(3), 413-425. doi:10.1093/biolre/iox095

The aim of this study was to test the hypothesis that the metabolic stresses associated with lactation alter the ability of the endometrium to respond appropriately to the conceptus by examining endometrial gene expression on Day 19 of pregnancy. Imm... Read More

Crossroads between peripheral atherosclerosis, western-type diet and skeletal muscle pathophysiology: emphasis on apolipoprotein E deficiency and peripheral arterial disease (2017)
Journal Article
Sfyri, P., & Matsakas, A. (2017). Crossroads between peripheral atherosclerosis, western-type diet and skeletal muscle pathophysiology: emphasis on apolipoprotein E deficiency and peripheral arterial disease. Journal of Biomedical Science, 24(1), doi:10.1186/s12929-017-0346-8

Atherosclerosis is a chronic inflammatory process that, in the presence of hyperlipidaemia, promotes the formation of atheromatous plaques in large vessels of the cardiovascular system. It also affects peripheral arteries with major implications for... Read More

Bleomycin increases neutrophil adhesion to human vascular endothelial cells independently of upregulation of ICAM-1 and E-selectin (2016)
Journal Article
Crooks, M. G., Greenman, J., Hart, S. P., Sadofsky, L. R., & Williamson, J. D. (2016). Bleomycin increases neutrophil adhesion to human vascular endothelial cells independently of upregulation of ICAM-1 and E-selectin. Experimental Lung Research, 42(8-10), 397-407. doi:10.1080/01902148.2016.1243742

© 2016 Taylor & Francis. Aim of the Study: Bleomycin-induced lung disease is a serious complication of therapy characterized by alveolar injury, cytokine release, inflammatory cell recruitment, and eventually pulmonary fibrosis. The mechanisms unde... Read More