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Dr Leonid Nikitenko's Outputs (35)

Calcitonin gene-related peptide and intermedin induce phosphorylation of p44/42 MAPK in primary human lymphatic endothelial cells in vitro (2024)
Journal Article
Hasan, S. R., Manolis, D., Stephenson, E., Ryskiewicz-Sokalska, O. A., Maraveyas, A., & Nikitenko, L. L. (2024). Calcitonin gene-related peptide and intermedin induce phosphorylation of p44/42 MAPK in primary human lymphatic endothelial cells in vitro. Cellular Signalling, 121, Article 111261. https://doi.org/10.1016/j.cellsig.2024.111261

Calcitonin gene-related peptide (CGRP) and adrenomedullin 2/intermedin (AM2/IMD) play important roles in several pathologies, including cardiovascular disease, migraine and cancer. The efficacy of drugs targeting CGRP signalling axis for the treatmen... Read More about Calcitonin gene-related peptide and intermedin induce phosphorylation of p44/42 MAPK in primary human lymphatic endothelial cells in vitro.

Quantitative proteomics reveals CLR interactome in primary human cells (2024)
Journal Article
Manolis, D., Hasan, S., Maraveyas, A., O'Brien, D. P., Kessler, B. M., Kramer, H., & Nikitenko, L. L. (2024). Quantitative proteomics reveals CLR interactome in primary human cells. Journal of Biological Chemistry, 300(6), Article 107399. https://doi.org/10.1016/j.jbc.2024.107399

The G protein–coupled receptor (GPCR) calcitonin receptor–like receptor (CLR) mediates essential functions in several cell types and is implicated in cardiovascular pathologies, skin diseases, migraine, and cancer. To date, the network of proteins in... Read More about Quantitative proteomics reveals CLR interactome in primary human cells.

Investigation of Aged Human Lung Endothelial Cells in Idiopathic Pulmonary Fibrosis (2024)
Thesis
Faulkner, E. C. Investigation of Aged Human Lung Endothelial Cells in Idiopathic Pulmonary Fibrosis. (Thesis). University of Hull. https://hull-repository.worktribe.com/output/4866422

Introduction
Older age is the main risk factor for idiopathic pulmonary fibrosis (IPF). Ageing-related changes in lung blood (pulmonary and bronchial) and lymphatic vessels are implicated in IPF pathophysiology, based on reports of their altered den... Read More about Investigation of Aged Human Lung Endothelial Cells in Idiopathic Pulmonary Fibrosis.

Abstract A020: Calcitonin receptor-like receptor is expressed in blood vessels in clear cell renal cell carcinoma and upregulated in endothelial cells co-cultured with tumor cells (2023)
Presentation / Conference Contribution
Morfitt, M. A., Greenman, J., Maraveyas, A., Harris, A. L., & Nikitenko, L. L. Abstract A020: Calcitonin receptor-like receptor is expressed in blood vessels in clear cell renal cell carcinoma and upregulated in endothelial cells co-cultured with tumor cells. Presented at AACR Special Conference on Advances in Kidney Cancer Research, Austin, Texas, U.S.A

Introduction. Clear cell renal cell carcinoma (ccRCC) is a highly vascularized and clinically aggressive kidney cancer, with high rates of metastasis and recurrence after cytoreductive nephrectomy. ccRCC can be treated with anti-angiogenic drugs, how... Read More about Abstract A020: Calcitonin receptor-like receptor is expressed in blood vessels in clear cell renal cell carcinoma and upregulated in endothelial cells co-cultured with tumor cells.

Abstract B001: Calcitonin receptor-like receptor agonists induce p44/42 MAPK phosphorylation in VEGF-A-stimulated human blood endothelial cells after bevacizumab treatment (2023)
Presentation / Conference Contribution
Morfitt, M. A., Greenman, J., Maraveyas, A., & Nikitenko, L. L. Abstract B001: Calcitonin receptor-like receptor agonists induce p44/42 MAPK phosphorylation in VEGF-A-stimulated human blood endothelial cells after bevacizumab treatment. Presented at AACR Special Conference: Advances in Kidney Cancer Research, Austin, Texas, U.S.A

Introduction. Clear cell renal cell carcinoma (ccRCC) is a highly vascularized and clinically aggressive cancer. In the UK, there are around 9000 diagnoses and 3000 deaths annually. Targeted therapies that inhibit angiogenic regulators, such as bevac... Read More about Abstract B001: Calcitonin receptor-like receptor agonists induce p44/42 MAPK phosphorylation in VEGF-A-stimulated human blood endothelial cells after bevacizumab treatment.

Biochemical characterisation of the interaction of the myosin light chain phosphatase with the regulatory subunits of protein kinase A in cells of the circulatory system (2023)
Thesis
Saldanha, P. A. C. B. D. S. Biochemical characterisation of the interaction of the myosin light chain phosphatase with the regulatory subunits of protein kinase A in cells of the circulatory system. (Thesis). Hull York Medical School. https://hull-repository.worktribe.com/output/4790706

Regulation of actomyosin-driven contraction is important for numerous cardiovascular cell functions, including smooth muscle tone, endothelial cell permeability and platelet shape change and spreading. These processes are primarily regulated by the p... Read More about Biochemical characterisation of the interaction of the myosin light chain phosphatase with the regulatory subunits of protein kinase A in cells of the circulatory system.

Quantitative proteomics of pancreatic cyst fluid for early diagnosis of cancer. (2023)
Presentation / Conference Contribution
Nikitenko, L. L., Manolis, D., O'Brien, D. P., Adekeye, A., Kessler, B. M., Collins, C., Kramer, H., Chang, D., & Maraveyas, A. Quantitative proteomics of pancreatic cyst fluid for early diagnosis of cancer. Presented at The London Pancreas Workshop 2023, London, United Kingdom

In our EARLY DIAgnosis of PAncreatic Cancer (EARLY DIAPAC) study, we aim to analyse molecular changes or “signatures” associated with early stages of pancreatic carcinogenesis by using the combination of: (i) Label-Free Quantitative Proteomics (LFQP)... Read More about Quantitative proteomics of pancreatic cyst fluid for early diagnosis of cancer..

Biological function and clinical implication of coagulation proteins during malignant transformation of pancreatic cells (2023)
Thesis
Haque, F. Biological function and clinical implication of coagulation proteins during malignant transformation of pancreatic cells. (Thesis). Hull York Medical School. https://hull-repository.worktribe.com/output/4371279

The premalignant pancreatic cellular genotype can remain stable for years before rapid malignant transformation, often associated with inflammation. Tissue factor (TF) is an inflammatory modulator regulated by factor VIIa (fVIIa) for its levels and a... Read More about Biological function and clinical implication of coagulation proteins during malignant transformation of pancreatic cells.

Abstract 224: Establishing de novo platform for whole genome sequencing of pancreatic cyst fluid for early detection and diagnosis of pancreatic cancer (2023)
Presentation / Conference Contribution
Adekeye, A., Haque, F., Ettelaie, C., Nikitenko, L., & Maraveyas, A. Abstract 224: Establishing de novo platform for whole genome sequencing of pancreatic cyst fluid for early detection and diagnosis of pancreatic cancer. Presented at AACR Annual Meeting 2023, Orlando, Florida

Background: Pancreatic cancer (PC) is a leading cause of cancer-related deaths globally. Accurate PC detection at early or premalignant stage, when surgery is effective, would increase survival rates and prevent unnecessary surgery or surveillance. A... Read More about Abstract 224: Establishing de novo platform for whole genome sequencing of pancreatic cyst fluid for early detection and diagnosis of pancreatic cancer.

Corrigendum: MEF2 transcription factors are key regulators of sprouting angiogenesis (2022)
Journal Article
Sacilotto, N., Chouliaras, K. M., Nikitenko, L. L., Lu, Y. W., Fritzsche, M., Wallace, M. D., Nornes, S., García-Moreno, F., Payne, S., Bridges, E., Liu, K., Biggs, D., Ratnayaka, I., Herbert, S. P., Molnár, Z., Harris, A. L., Davies, B., Bond, G. L., Bou-Gharios, G., Schwarz, J. J., & De Val, S. (2022). Corrigendum: MEF2 transcription factors are key regulators of sprouting angiogenesis. Genes & development, 36(19-20), 1096. https://doi.org/10.1101/gad.350249.122

The above-mentioned article contained three errors in the Supplemental Figures. In Supplemental Figure 3D, both bar graphs are missing labels for the X-axes due to an oversight during figure preparation.

Complex Transcriptional Profiles of the PPP1R12A Gene in Cells of the Circulatory System as Revealed by In Silico Analysis and Reverse Transcription PCR (2022)
Journal Article
Saldanha, P. A., Bolanle, I. O., Palmer, T. M., Nikitenko, L. L., & Rivero, F. (2022). Complex Transcriptional Profiles of the PPP1R12A Gene in Cells of the Circulatory System as Revealed by In Silico Analysis and Reverse Transcription PCR. Cells, 11(15), Article 2315. https://doi.org/10.3390/cells11152315

The myosin light chain phosphatase target subunit 1 (MYPT1), encoded by the PPP1R12A gene, is a key component of the myosin light chain phosphatase (MLCP) protein complex. MYPT1 isoforms have been described as products of the cassette-type alternativ... Read More about Complex Transcriptional Profiles of the PPP1R12A Gene in Cells of the Circulatory System as Revealed by In Silico Analysis and Reverse Transcription PCR.

Integrated single-cell RNA sequencing analysis reveals alterations of ageing human lung endothelium heterogeneity in idiopathic pulmonary fibrosis (2022)
Preprint / Working Paper
Faulkner, E. C., Moverley, A. A., Hart, S. P., & Nikitenko, L. L. Integrated single-cell RNA sequencing analysis reveals alterations of ageing human lung endothelium heterogeneity in idiopathic pulmonary fibrosis

Increasing age is the main risk factor for chronic lung diseases (CLD) including idiopathic pulmonary fibrosis (IPF). Halting or reversing progression of IPF remains an unmet clinical need due to limited knowledge of underlying mechanisms. In particu... Read More about Integrated single-cell RNA sequencing analysis reveals alterations of ageing human lung endothelium heterogeneity in idiopathic pulmonary fibrosis.

Low molecular weight heparin and direct oral anticoagulants influence tumour formation, growth, invasion and vascularisation by separate mechanisms (2019)
Journal Article
Featherby, S., Xiao, Y. P., Ettelaie, C., Nikitenko, L. L., Greenman, J., & Maraveyas, A. (2019). Low molecular weight heparin and direct oral anticoagulants influence tumour formation, growth, invasion and vascularisation by separate mechanisms. Scientific reports, 9(1), Article 6272. https://doi.org/10.1038/s41598-019-42738-1

The bidirectional association between coagulation and cancer has been established. However, anticoagulant therapies have been reported to have beneficial outcomes by influencing the vascularisation of the tumours. In this study the influence of a set... Read More about Low molecular weight heparin and direct oral anticoagulants influence tumour formation, growth, invasion and vascularisation by separate mechanisms.

MEF2 transcription factors are key regulators of sprouting angiogenesis (2016)
Journal Article
Sacilotto, N., Chouliaras, K. M., Nikitenko, L. L., Lu, Y. W., Fritzsche, M., Wallace, M. D., Nornes, S., García-Moreno, F., Payne, S., Bridges, E., Liu, K., Biggs, D., Ratnayaka, I., Herbert, S. P., Molnár, Z., Harris, A. L., Davies, B., Bond, G. L., Bou-Gharios, G., Schwarz, J. J., & De Val, S. (2016). MEF2 transcription factors are key regulators of sprouting angiogenesis. Genes & development, 30(20), 2297-2309. https://doi.org/10.1101/gad.290619.116

© 2016 Sacilotto et al. Angiogenesis, the fundamental process by which new blood vessels form from existing ones, depends on precise spatial and temporal gene expression within specific compartments of the endothelium. However, the molecular links be... Read More about MEF2 transcription factors are key regulators of sprouting angiogenesis.

The G-protein-coupled receptor CLR is upregulated in an autocrine loop with adrenomedullin in clear cell renal cell carcinoma and associated with poor prognosis (2013)
Journal Article
Nikitenko, L. L., Leek, R., Henderson, S., Pillay, N., Turley, H., Generali, D., Gunningham, S., Morrin, H. R., Pellagatti, A., Rees, M. C., Harris, A. L., & Fox, S. B. (2013). The G-protein-coupled receptor CLR is upregulated in an autocrine loop with adrenomedullin in clear cell renal cell carcinoma and associated with poor prognosis. Clinical cancer research : an official journal of the American Association for Cancer Research, 19(20), 5740-5748. https://doi.org/10.1158/1078-0432.CCR-13-1712

Purpose: The G-protein-coupled receptor (GPCR) calcitonin receptor-like receptor (CLR) and its ligand peptide adrenomedullin (encoded by ADM gene) are implicated in tumor angiogenesis in mouse models but poorly defined in human cancers. We therefore... Read More about The G-protein-coupled receptor CLR is upregulated in an autocrine loop with adrenomedullin in clear cell renal cell carcinoma and associated with poor prognosis.

Adrenomedullin haploinsufficiency predisposes to secondary lymphedema (2013)
Journal Article
Nikitenko, L. L., Shimosawa, T., Henderson, S., Mäkinen, T., Shimosawa, H., Qureshi, U., Pedley, R. B., Rees, M. C., Fujita, T., & Boshoff, C. (2013). Adrenomedullin haploinsufficiency predisposes to secondary lymphedema. Journal of Investigative Dermatology, 133(7), 1768-1776. https://doi.org/10.1038/jid.2013.47

Secondary lymphedema is a debilitating condition, and genetic factors predisposing to its development remain largely unknown. Adrenomedullin (AM) is peptide encoded, together with proadrenomedullin N-terminal peptide (PAMP), by the Adm gene (adrenome... Read More about Adrenomedullin haploinsufficiency predisposes to secondary lymphedema.

The expression and regulation of adrenomedullin in the human endometrium: A candidate for endometrial repair (2011)
Journal Article
Maybin, J. A., Battersby, S., Hirani, N., Nikitenko, L. L., Critchley, H. O., & Jabbour, H. N. (2011). The expression and regulation of adrenomedullin in the human endometrium: A candidate for endometrial repair. Endocrinology, 152(7), 2845-2856. https://doi.org/10.1210/en.2010-1256

After menstruation, the endometrium has a remarkable capacity for repair, but the factors involved remain undefined. We hypothesize adrenomedullin (AM) plays a role in this process. Premenstrually progesterone levels decline, stimulating prostaglandi... Read More about The expression and regulation of adrenomedullin in the human endometrium: A candidate for endometrial repair.

KSHV-encoded miRNAs target MAF to induce endothelial cell reprogramming (2010)
Journal Article
Hansen, A., Henderson, S., Lagos, D., Nikitenko, L., Coulter, E., Roberts, S., Gratrix, F., Plaisance, K., Renne, R., Bower, M., Kellam, P., & Boshoff, C. (2010). KSHV-encoded miRNAs target MAF to induce endothelial cell reprogramming. Genes & development, 24(2), 195-205. https://doi.org/10.1101/gad.553410

Kaposi sarcoma herpesvirus (KSHV) induces transcriptional reprogramming of endothelial cells. In particular, KSHV-infected lymphatic endothelial cells (LECs) show an up-regulation of genes associated with blood vessel endothelial cells (BECs). Conseq... Read More about KSHV-encoded miRNAs target MAF to induce endothelial cell reprogramming.