TSB Costs (Study 1, 2, 3 & 4): In vitro and in vivo assessment of polyphenol extracts on cardiovascular indices

Project Description

Evidence suggests dietary plant polyphenols are associated with reducing indices of cardiovascular disease such as endothelial dysfunction, insulin resistance and hypertension. The key objectives of this translational research proposal are to study and optimise the varietal activities of orally delivered polyphenol preparations, understand the influence of phase II metabolism on these activities, and link activity with cardiovascular tone, focusing on endogenous formation of three key reactive oxygen species (ROS). The proposal will define the optimum matrix for polyphenol bioavailability andbioactivity in vitro, looking at the influcence of circulating phase II metabolites on defined molecular mediators and markers of endothlial function. The bioavailability and pharmacokinetics of the preparations will then be characterised in vivo, and in an additional innovative step these parameters will be shown to be enhanced using pollen shell (exine) technology. A blinded clinical trial will be undertaken to evaluate the use of polyphenols in modyfying and improving glucose control and indices of endothelial function and cardiovascular risk in type 2 diabetes.