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Imaging immunoassay in negative: Surface-­‐catalysed chemiluminescence for the detection of pregnancy hormones in artificial saliva

Wright, Kevin J.; Oiaidha, Zeid O.; Love, Daniel P.; Aljohani, Mohammed; Greenway, Gillian M.; Wadhawan, Jay D.

Authors

Kevin J. Wright

Zeid O. Oiaidha

Daniel P. Love

Mohammed Aljohani

Gillian M. Greenway



Contributors

Abstract

A novel and rapid (45min), quantitative chemiluminescence-based, surface immunoassay is reported for the quantitative detection of progesterone and œstriol in artificial saliva. The detection limits for these pregnancy hormones are 2.3 and 2.5 pg mL-1, respectively. The assay is based on the use of ferrocene- tagged, monoclonal antibodies immobilised on a surface, so that the oxidised ferricenium catalyses the reaction between luminol and hydrogen peroxide. The immunoassay is performed in negative, such that increasing the antigen concentration gives rise to decreasing light intensity that is observed, and is unaffected by antibody orientation on the surface. This affords a method of calibration that is readily translated to pregnancy hormone detection in a primary point-of-care environment. Biomolecules with similar structures to these pregnancy hormones found in saliva are demonstrated not to interfere with the immunoassay.

Journal Article Type Article
Publication Date Oct 12, 2018
Print ISSN 1144-0546
Electronic ISSN 1369-9261
Publisher Royal Society of Chemistry
Peer Reviewed Peer Reviewed
Volume 42
Issue 23
Pages 18641-18648
APA6 Citation Wright, K. J., Oiaidha, Z. O., Love, D. P., Aljohani, M., Greenway, G. M., & Wadhawan, J. D. (2018). Imaging immunoassay in negative: Surface-­‐catalysed chemiluminescence for the detection of pregnancy hormones in artificial saliva. New journal of chemistry = Nouveau journal de chimie, 42(23), 18641-18648. https://doi.org/10.1039/c8nj02974a
DOI https://doi.org/10.1039/c8nj02974a
Keywords Chemiluminescence, visual immunoassay, pregnancy hormones, luminol/hydrogen peroxide.
Publisher URL https://pubs.rsc.org/en/Content/ArticleLanding/2018/NJ/C8NJ02974A

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