Modelling antisepsis using defined populations of facultative and anaerobic wound pathogens grown in a basally perfused biofilm model
Oates, Angela; Lindsay, Sharon; Mistry, Hitesh; Ortega, Fernando; McBain, Andrew J.
Andrew J. McBain
An in vitro model was developed to assess the effects of topical antimicrobials on taxonomically defined wound biofilms. Biofilms were exposed over seven days to povidone-iodine, silver acetate or polyhexamethylene biguanide (PHMB) at concentrations used in wound dressings. The rank order of tolerance in multi-species biofilms, based on an analysis of the average bacterial counts over time was P. aeruginosa > methicillin-resistant Staphylococcus aureus (MRSA) > B. fragilis > S. pyogenes. The rank order of effectiveness for the antimicrobials in the biofilm model was povidone-iodine > PHMB > silver acetate. None of the test compounds eradicated P. aeruginosa or MRSA from the biofilms although all compounds except silver acetate eliminated S. pyogenes. Antimicrobial effectiveness against bacteria grown in multi-species biofilms did not correlate with planktonic susceptibility. Defined biofilm populations of mixed-species wound pathogens could be maintained in the basal perfusion model, facilitating the efficacy testing of treatments regimens and potential dressings against multi-species biofilms composed of wound isolates.
|Journal Article Type||Article|
|Publication Date||May 28, 2018|
|Publisher||Taylor & Francis|
|Peer Reviewed||Peer Reviewed|
|APA6 Citation||Oates, A., Lindsay, S., Mistry, H., Ortega, F., & McBain, A. J. (2018). Modelling antisepsis using defined populations of facultative and anaerobic wound pathogens grown in a basally perfused biofilm model. Biofouling, 34(5), 507-518. https://doi.org/10.1080/08927014.2018.1466115|
|Keywords||Aquatic Science; Applied Microbiology and Biotechnology; Water Science and Technology|
|Related Public URLs||http://eprints.whiterose.ac.uk/129665/|
|Additional Information||Peer Review Statement: The publishing and review policy for this title is described in its Aims & Scope.; Aim & Scope: http://www.tandfonline....cope&journalCode=gbif20|
©2019 The authors
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