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Modelling antisepsis using defined populations of facultative and anaerobic wound pathogens grown in a basally perfused biofilm model

Oates, Angela; Lindsay, Sharon; Mistry, Hitesh; Ortega, Fernando; McBain, Andrew J.

Authors

Angela Oates

Sharon Lindsay

Hitesh Mistry

Fernando Ortega

Andrew J. McBain



Abstract

An in vitro model was developed to assess the effects of topical antimicrobials on taxonomically defined wound biofilms. Biofilms were exposed over seven days to povidone-iodine, silver acetate or polyhexamethylene biguanide (PHMB) at concentrations used in wound dressings. The rank order of tolerance in multi-species biofilms, based on an analysis of the average bacterial counts over time was P. aeruginosa > methicillin-resistant Staphylococcus aureus (MRSA) > B. fragilis > S. pyogenes. The rank order of effectiveness for the antimicrobials in the biofilm model was povidone-iodine > PHMB > silver acetate. None of the test compounds eradicated P. aeruginosa or MRSA from the biofilms although all compounds except silver acetate eliminated S. pyogenes. Antimicrobial effectiveness against bacteria grown in multi-species biofilms did not correlate with planktonic susceptibility. Defined biofilm populations of mixed-species wound pathogens could be maintained in the basal perfusion model, facilitating the efficacy testing of treatments regimens and potential dressings against multi-species biofilms composed of wound isolates.

Journal Article Type Article
Publication Date May 28, 2018
Journal Biofouling
Print ISSN 0892-7014
Electronic ISSN 1029-2454
Publisher Taylor & Francis
Peer Reviewed Peer Reviewed
Volume 34
Issue 5
Pages 507-518
APA6 Citation Oates, A., Lindsay, S., Mistry, H., Ortega, F., & McBain, A. J. (2018). Modelling antisepsis using defined populations of facultative and anaerobic wound pathogens grown in a basally perfused biofilm model. Biofouling, 34(5), 507-518. https://doi.org/10.1080/08927014.2018.1466115
DOI https://doi.org/10.1080/08927014.2018.1466115
Keywords Aquatic Science; Applied Microbiology and Biotechnology; Water Science and Technology
Publisher URL https://www.tandfonline.com/doi/full/10.1080/08927014.2018.1466115
Related Public URLs http://eprints.whiterose.ac.uk/129665/
Additional Information Peer Review Statement: The publishing and review policy for this title is described in its Aims & Scope.; Aim & Scope: http://www.tandfonline....cope&journalCode=gbif20

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