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Boosting the antimicrobial action of vancomycin formulated in shellac nanoparticles of dual-surface functionality

Al-Obaidy, Saba S.M.; Halbus, Ahmed F.; Greenway, Gillian M.; Paunov, Vesselin N.


Saba S.M. Al-Obaidy

Ahmed F. Halbus

Gillian M. Greenway

Vesselin N. Paunov


Saba A.M Al-Obaidy

Ahmed F. Halbus

Gillian M. Greenway

Vesselin N. Paunov


We report a strong amplification of the antimicrobial action of vancomycin (VCM) encapsulated in shellac nanoparticles (NPs) with dual surface functionalisation. These shellac nanocarriers for VCM were produced in two steps: (i) a pH drop from aqueous ammonium shellac solution containing Poloxamer 407 (P407) as a steric stabilising polymer in solution of vancomycin hydrochloride, and (ii) subsequent doping with the insoluble cationic surfactant octadecyltrimethylammonium bromide (ODTAB) though a solvent change to yield cationic surface functionality. We evaluated the encapsulation efficiency of VCM and its release profiles from these nanocarriers. This study explored the antibiotic action of these VCM nanocarriers at the various stages of their preparation which helped us to evaluate how they could be made to work efficiently, to adapt their design and demonstrate the role of the nanocarrier dual functionalisation on its antibiotic action and delivery. The antibiotic effect of VCM loaded in such versatile functionalised shellac nanocarriers was tested on three different proxy microorganisms, C. reinhardtii, S. cerevisiae and E. coli. We also compared the antibiotic effect of free VCM with non-coated VCM-loaded nanocarriers at the same overall concentrations. The ODTAB coating of the shellac NPs strongly enhanced the antibiotic action of the encapsulated VCM across all tested microorganisms. The enhanced VCM action is explained with the increased electrostatic adhesion between the ODTAB-coated VCM-loaded shellac NPs and the negatively charged surface of the microbial cell walls which allows local delivery of VCM with a high concentration directly on the cell membrane. This nanocarrier-mediated boost of the antibiotic action may potentially breathe new life into old antibiotics and help to fight off antibiotic resistance by making them more effective.


Al-Obaidy, S. S., Halbus, A. F., Greenway, G. M., & Paunov, V. N. (2019). Boosting the antimicrobial action of vancomycin formulated in shellac nanoparticles of dual-surface functionality. Journal of Materials Chemistry B, 7(19), 3119-3133.

Journal Article Type Article
Acceptance Date Apr 9, 2019
Online Publication Date Apr 15, 2019
Publication Date 2019
Deposit Date May 21, 2019
Publicly Available Date Oct 27, 2022
Journal Journal of Materials Chemistry B
Print ISSN 2050-750X
Electronic ISSN 2050-750X
Publisher Royal Society of Chemistry
Peer Reviewed Peer Reviewed
Volume 7
Issue 19
Pages 3119-3133
Keywords General Materials Science; General Chemistry; Biomedical Engineering; General Medicine
Public URL
Publisher URL
Additional Information This is the accepted manuscript of an article published in Journal of materials chemistry B, 2019. The version of record is available at the DOI link in this record.


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