Gene expression and metabolic response of bovine oviduct epithelial cells to the early embryo

Hamdi, Meriem; Sánchez-Calabuig, María J; Rodríguez-Alonso, Beatriz; Bagés-Arnal, Sandra; Roussi, Kalliopi; Sturmey, Roger; Gutiérrez-Adán, Alfonso; Lonergan, Patrick; Rizos, Dimitrios

Abstract

© 2019 Society for Reproduction and Fertility During its journey through the oviduct, the bovine embryo may induce transcriptomic and metabolic responses, via direct or indirect contact, from bovine oviduct epithelial cells (BOECs). An in vitro model using polyester mesh was established, allowing the study of the local contact during 48 h between a BOEC monolayer and early embryos (2- or 8-cell stage) or their respective conditioned media (CM). The transcriptomic response of BOEC to early embryos was assessed by analyzing the transcript abundance of SMAD6, TDGF1, ROCK1, ROCK2, SOCS3, PRELP and AGR3 selected from previous in vivo studies and GPX4, NFE2L2, SCN9A, EPSTI1 and IGFBP3 selected from in vitro studies. Moreover, metabolic analyses were performed on the media obtained from the co-culture. Results revealed that presence of early embryos or their CM altered the BOEC expression of NFE2L2, GPX4, SMAD6, IGFBP3, ROCK2 and SCN9A. However, the response of BOEC to two-cell embryos or their CM was different from that observed to eight-cell embryos or their CM. Analysis of energy substrates and amino acids revealed that BOEC metabolism was not affected by the presence of early embryos or by their CM. Interestingly, embryo metabolism before embryo genome activation (EGA) seems to be independent of exogenous sources of energy. In conclusion, this study confirms that early embryos affect BOEC transcriptome and BOEC response was embryo stage specific. Moreover, embryo affects BOEC via a direct contact or via its secretions. However transcriptomic response of BOEC to the embryo did not manifest as an observable metabolic response.

Citation

Hamdi, M., Sánchez-Calabuig, M. J., Rodríguez-Alonso, B., Bagés-Arnal, S., Roussi, K., Sturmey, R., Gutiérrez-Adán, A., Lonergan, P., & Rizos, D. (2019). Gene expression and metabolic response of bovine oviduct epithelial cells to the early embryo. Reproduction, 158(1), 85-94. https://doi.org/10.1530/REP-18-0561

• Journal Article Type: Article
• Acceptance Date: Apr 25, 2019
• Online Publication Date: Apr 25, 2019
• Publication Date: 2019-07
• Deposit Date: Apr 30, 2019
• Publicly Available Date: Apr 26, 2020
• Journal: Reproduction
• Print ISSN: 1470-1626
• Publisher: BioScientifica
• Peer Reviewed: Peer Reviewed
• Volume: 158
• Issue: 1
• Pages: 85-94
• DOI: https://doi.org/10.1530/REP-18-0561
• Public URL: https://hull-repository.worktribe.com/output/1681298
• Publisher URL: https://rep.bioscientifica.com/view/journals/rep/aop/rep-18-0561.xml
• Additional Information: This manuscript has been accepted for publication in Reproduction, but the version presented here has not yet been copy-edited, formatted or proofed. Consequently, Bioscientifica accepts no responsibility for any errors or omissions it may contain. The definitive version is now freely available at https://doi.org/10.1530/REP-18-0561 2019
• Contract Date: Apr 30, 2019