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Gene expression and metabolic response of bovine oviduct epithelial cells to the early embryo

Hamdi, Meriem; Sánchez-Calabuig, María J; Rodríguez-Alonso, Beatriz; Bagés-Arnal, Sandra; Roussi, Kalliopi; Sturmey, Roger; Gutiérrez-Adán, Alfonso; Lonergan, Patrick; Rizos, Dimitrios

Authors

Meriem Hamdi

María J Sánchez-Calabuig

Beatriz Rodríguez-Alonso

Sandra Bagés-Arnal

Kalliopi Roussi

Alfonso Gutiérrez-Adán

Patrick Lonergan

Dimitrios Rizos



Abstract

During its journey through the oviduct, the bovine embryo may induce transcriptomic and metabolic responses, via direct or indirect contact, from bovine oviduct epithelial cells (BOEC). An in vitro model using polyester mesh was established, allowing the study of the local contact during 48 h between a BOEC monolayer and early embryos (2- or 8-cell stage) or their respective conditioned media (CM). The transcriptomic response of BOEC to early embryos was assessed by analyzing the transcript abundance of SMAD6, TDGF1, ROCK1, ROCK2, SOCS3, PRELP and AGR3 selected from previous in vivo studies and GPX4, NFE2L2, SCN9A, EPSTI1, IGFBP3 selected from in vitro studies. Moreover, metabolic analyses were performed on the media obtained from the co-culture. Results revealed that presence of early embryos or their CM altered the BOEC expression of NFE2L2, GPX4, SMAD6, IGFBP3, ROCK2 and SCN9A. However, the response of BOEC to 2-cell embryos or their CM was different from that observed to 8-cell embryos or their CM. Analysis of energy substrates and amino acids revealed that BOEC metabolism was not affected by the presence of early embryos or by their CM. Interestingly, embryo metabolism before embryo genome activation (EGA) seems to be independent of exogenous sources of energy. In conclusion, this study confirms that early embryos affect BOEC transcriptome and BOEC response was embryo stage-specific. Moreover, embryo affects BOEC via a direct contact or via its secretions. However transcriptomic response of BOEC to the embryo did not manifest as an observable metabolic response.

Journal Article Type Article
Publication Date 2019-07
Journal Reproduction
Print ISSN 1470-1626
Electronic ISSN 1741-7899
Publisher BioScientifica
Peer Reviewed Peer Reviewed
Volume 158
Issue 1
Pages 85-94
Institution Citation Hamdi, M., Sánchez-Calabuig, M. J., Rodríguez-Alonso, B., Bagés-Arnal, S., Roussi, K., Sturmey, R., …Rizos, D. (2019). Gene expression and metabolic response of bovine oviduct epithelial cells to the early embryo. Reproduction, 158(1), 85-94. https://doi.org/10.1530/REP-18-0561
DOI https://doi.org/10.1530/REP-18-0561
Publisher URL https://rep.bioscientifica.com/view/journals/rep/aop/rep-18-0561.xml
Additional Information This manuscript has been accepted for publication in Reproduction, but the version presented here has not yet been copy-edited, formatted or proofed. Consequently, Bioscientifica accepts no responsibility for any errors or omissions it may contain. The definitive version is now freely available at https://doi.org/10.1530/REP-18-0561 2019