Osama M. Alshehri
Activation of glycoprotein VI (GPVI) and C-type lectin-like receptor-2 (CLEC-2) underlies platelet activation by diesel exhaust particles and other charged/hydrophobic ligands
Alshehri, Osama M.; Montague, Samantha; Watson, Stephanie; Carter, Paul; Sarker, Najiat; Manne, Bhanu K.; Miller, Jeanette L.C.; Herr, Andrew B.; Pollitt, Alice Y.; O'Callaghan, Chris A.; Kunapuli, Satya; Arman, Monica; Hughes, Craig E.; Watson, Steve P.
Bhanu K. Manne
Jeanette L.C. Miller
Andrew B. Herr
Alice Y. Pollitt
Chris A. O'Callaghan
Craig E. Hughes
Steve P. Watson
Platelets are activated by a range of stimuli that share little or no resemblance in structure to each other or to recognized ligands, including diesel exhaust particles (DEP), small peptides [4N1-1, Champs (computed helical anti-membrane proteins), LSARLAF (Leu-Ser-Ala-Arg-Leu-Ala-Phe)], proteins (histones) and large polysaccharides (fucoidan, dextran sulfate). This miscellaneous group stimulate aggregation of human and mouse platelets through the glycoproteinVI (GPVI)-FcR γ -chain complex and/or C-type lectin-like receptor-2 (CLEC-2) as shown using platelets from mice deficient in either or both of these receptors. In addition, all of these ligands stimulate tyrosine phosphorylation in GPVI/CLEC-2-double-deficient platelets, indicating that they bind to additional surface receptors, although only in the case of dextran sulfate does this lead to activation. DEP, fucoidan and dextran sulfate, but not the other agonists, activate GPVI and CLEC-2 in transfected cell lines as shown using a sensitive reporter assay confirming a direct interaction with the two receptors. We conclude that this miscellaneous group of ligands bind to multiple proteins on the cell surface including GPVI and/or CLEC-2, inducing activation. These results have pathophysiological significance in a variety of conditions that involve exposure to activating charged/hydrophobic agents.
Alshehri, O. M., Montague, S., Watson, S., Carter, P., Sarker, N., Manne, B. K., …Watson, S. P. (2015). Activation of glycoprotein VI (GPVI) and C-type lectin-like receptor-2 (CLEC-2) underlies platelet activation by diesel exhaust particles and other charged/hydrophobic ligands. Biochemical Journal, 468(3), 459-473. https://doi.org/10.1042/BJ20150192
|Journal Article Type||Article|
|Acceptance Date||Apr 7, 2015|
|Online Publication Date||Jun 15, 2015|
|Publication Date||Jun 15, 2015|
|Deposit Date||May 22, 2019|
|Peer Reviewed||Peer Reviewed|
|Keywords||C-type lectin-like receptor-2 (CLEC-2); glycoprotein VI (GPVI); histones; Src; Syk; tyrosine kinase|
This file is under embargo due to copyright reasons.
Contact M.Arman-Albert@hull.ac.uk to request a copy for personal use.
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