Harvesting human prostate tissue material and culturing primary prostate epithelial cells

Frame, Fiona M.; Pellacani, Davide; Collins, Anne T.; Maitland, Norman J.

Abstract

In order to fully explore the biology of a complex solid tumor such as prostate cancer, it is desirable to work with patient tissue. Only by working with cells from a tissue can we take into account patient variability and tumor heterogeneity. Cell lines have long been regarded as the workhorse of cancer research and it could be argued that they are of most use when considered within a panel of cell lines, thus taking into account specified mutations and variations in phenotype between different cell lines. However, often very different results are obtained when comparing cell lines to primary cells cultured from tissue. It stands to reason that cells cultured from patient tissue represents a close-to-patient model that should and does produce clinically relevant data. This chapter aims to illustrate the methods of processing, storing and culturing cells from prostate tissue, with a description of potential uses.

Citation

Frame, F. M., Pellacani, D., Collins, A. T., & Maitland, N. J. (2016). Harvesting human prostate tissue material and culturing primary prostate epithelial cells. In I. J. McEwan (Ed.), The nuclear receptor superfamily: methods and protocols (181-201). New York, NY: Humana Press. https://doi.org/10.1007/978-1-4939-3724-0_12

• Acceptance Date: Feb 23, 2015
• Online Publication Date: Jun 1, 2016
• Publication Date: 2016
• Deposit Date: Jun 20, 2019
• Journal: Methods in Molecular Biology; The Nuclear Receptor Superfamily
• Print ISSN: 1064-3745
• Electronic ISSN: 1940-6029
• Pages: 181-201
• Series Title: Methods in Molecular Biology
• Series Number: 1443
• Book Title: The nuclear receptor superfamily: methods and protocols
• ISBN: 978-1-4939-3722-6
• DOI: https://doi.org/10.1007/978-1-4939-3724-0_12
• Keywords: Prostate; Tissue; Primary epithelial cells; Cancer; Benign prostatic hyperplasia
• Public URL: https://hull-repository.worktribe.com/output/2020271
• Publisher URL: https://link.springer.com/protocol/10.1007%2F978-1-4939-3724-0_12