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Harvesting human prostate tissue material and culturing primary prostate epithelial cells

Frame, Fiona M.; Pellacani, Davide; Collins, Anne T.; Maitland, Norman J.

Authors

Fiona M. Frame

Davide Pellacani

Anne T. Collins

Norman J. Maitland



Contributors

Fiona Frame
Researcher

Iain J. McEwan
Editor

Abstract

In order to fully explore the biology of a complex solid tumor such as prostate cancer, it is desirable to work with patient tissue. Only by working with cells from a tissue can we take into account patient variability and tumor heterogeneity. Cell lines have long been regarded as the workhorse of cancer research and it could be argued that they are of most use when considered within a panel of cell lines, thus taking into account specified mutations and variations in phenotype between different cell lines. However, often very different results are obtained when comparing cell lines to primary cells cultured from tissue. It stands to reason that cells cultured from patient tissue represents a close-to-patient model that should and does produce clinically relevant data. This chapter aims to illustrate the methods of processing, storing and culturing cells from prostate tissue, with a description of potential uses.

Citation

Frame, F. M., Pellacani, D., Collins, A. T., & Maitland, N. J. (2016). Harvesting human prostate tissue material and culturing primary prostate epithelial cells. In I. J. McEwan (Ed.), The nuclear receptor superfamily: methods and protocols (181-201). Humana Press. https://doi.org/10.1007/978-1-4939-3724-0_12

Online Publication Date Jun 1, 2016
Publication Date 2016
Deposit Date Jun 20, 2019
Journal Methods in Molecular Biology; The Nuclear Receptor Superfamily
Print ISSN 1064-3745
Electronic ISSN 1940-6029
Pages 181-201
Series Title Methods in Molecular Biology
Series Number 1443
Book Title The nuclear receptor superfamily: methods and protocols
ISBN 978-1-4939-3722-6
DOI https://doi.org/10.1007/978-1-4939-3724-0_12
Keywords Prostate; Tissue; Primary epithelial cells; Cancer; Benign prostatic hyperplasia
Public URL https://hull-repository.worktribe.com/output/2020271
Publisher URL https://link.springer.com/protocol/10.1007%2F978-1-4939-3724-0_12
Contract Date Feb 23, 2015