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Low-temperature plasma treatment induces DNA damage leading to necrotic cell death in primary prostate epithelial cells

Hirst, A M; Simms, M S; Mann, V M; Maitland, N J; O'Connell, D; Frame, F M

Authors

A M Hirst

M S Simms

V M Mann

N J Maitland

D O'Connell

F M Frame



Abstract

Background:
In recent years, the rapidly advancing field of low-temperature atmospheric pressure plasmas has shown considerable promise for future translational biomedical applications, including cancer therapy, through the generation of reactive oxygen and nitrogen species.

Method:
The cytopathic effect of low-temperature plasma was first verified in two commonly used prostate cell lines: BPH-1 and PC-3 cells. The study was then extended to analyse the effects in paired normal and tumour (Gleason grade 7) prostate epithelial cells cultured directly from patient tissue. Hydrogen peroxide (H2O2) and staurosporine were used as controls throughout.

Results:
Low-temperature plasma (LTP) exposure resulted in high levels of DNA damage, a reduction in cell viability, and colony-forming ability. H2O2 formed in the culture medium was a likely facilitator of these effects. Necrosis and autophagy were recorded in primary cells, whereas cell lines exhibited apoptosis and necrosis.

Conclusions:
This study demonstrates that LTP treatment causes cytotoxic insult in primary prostate cells, leading to rapid necrotic cell death. It also highlights the need to study primary cultures in order to gain more realistic insight into patient response.

Citation

Hirst, A. M., Simms, M. S., Mann, V. M., Maitland, N. J., O'Connell, D., & Frame, F. M. (2015). Low-temperature plasma treatment induces DNA damage leading to necrotic cell death in primary prostate epithelial cells. The British Journal of Cancer, 112(9), 1536-1545. https://doi.org/10.1038/bjc.2015.113

Journal Article Type Article
Acceptance Date Mar 3, 2015
Online Publication Date Apr 2, 2015
Publication Date Apr 28, 2015
Deposit Date Jun 25, 2019
Publicly Available Date Mar 28, 2024
Journal British Journal of Cancer
Print ISSN 0007-0920
Electronic ISSN 1532-1827
Publisher Nature Publishing Group
Peer Reviewed Peer Reviewed
Volume 112
Issue 9
Pages 1536-1545
DOI https://doi.org/10.1038/bjc.2015.113
Keywords Cancer Research; Oncology
Public URL https://hull-repository.worktribe.com/output/2037316
Publisher URL https://www.nature.com/articles/bjc2015113
Additional Information This work is licensed under the Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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