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Differential cytotoxic activity of a novel palladium-based compound on prostate cell lines, primary prostate epithelial cells and prostate stem cells

Ulukaya, Engin; Frame, Fiona M.; Cevatemre, Buse; Pellacani, Davide; Walker, Hannah; Mann, Vincent M.; Simms, Matthew S.; Stower, Michael J.; Yilmaz, Veysel T.; Maitland, Norman J.

Authors

Engin Ulukaya

Buse Cevatemre

Davide Pellacani

Hannah Walker

Vincent M. Mann

Matthew S. Simms

Michael J. Stower

Veysel T. Yilmaz

Norman J. Maitland

Abstract

The outcome for patients with advanced metastatic and recurrent prostate cancer is still poor. Therefore, new chemotherapeutics are required, especially for killing cancer stem cells that are thought to be responsible for disease recurrence. In this study, we screened the effect of a novel palladium-based anticancer agent (Pd complex) against six different prostate cancer cell lines, and primary cultures from seven Gleason 6/7 prostate cancer, three Gleason 8/9 prostate cancer and four benign prostate hyperplasia patient samples, as well as cancer stem cells selected from primary cultures. MTT and ATP viability assays were used to assess cell growth and flow cytometry to assess cell cycle status. In addition, immunofluorescence was used to detect γH2AX nuclear foci, indicative of DNA damage, and Western blotting to assess the induction of apoptosis and autophagy. The Pd complex showed a powerful growth-inhibitory effect against both cell lines and primary cultures. More importantly, it successfully reduced the viability of cancer stem cells as first reported in this study. The Pd complex induced DNA damage and differentially induced evidence of cell death, as well as autophagy. In conclusion, this novel agent may be promising for use against the bulk of the tumour cell population as well as the prostate cancer stem cells, which are thought to be responsible for the resistance of metastatic prostate cancer to chemotherapy. This study also indicates that the combined use of the Pd complex with an autophagy modulator may be a more promising approach to treat prostate cancer. In addition, the differential effects observed between cell lines and primary cells emphasise the importance of the model used to test novel drugs including its genetic background, and indeed the necessity of using cells cultured from patient samples.

Journal Article Type Article
Publication Date May 10, 2013
Journal PLoS ONE
Print ISSN 1932-6203
Electronic ISSN 1932-6203
Publisher Public Library of Science
Peer Reviewed Peer Reviewed
Volume 8
Issue 5
Article Number e64278
Institution Citation Ulukaya, E., Frame, F. M., Cevatemre, B., Pellacani, D., Walker, H., Mann, V. M., …Maitland, N. J. (2013). Differential cytotoxic activity of a novel palladium-based compound on prostate cell lines, primary prostate epithelial cells and prostate stem cells. PloS one, 8(5), https://doi.org/10.1371/journal.pone.0064278
DOI https://doi.org/10.1371/journal.pone.0064278
Publisher URL https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0064278
Additional Information Copyright: © 2013 Ulukaya et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Copyright Statement
Copyright: © 2013 Ulukaya et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




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