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Development and limitations of lentivirus vectors as tools for tracking differentiation in prostate epithelial cells

Frame, Fiona M.; Hager, Stefanie; Pellacani, Davide; Stower, Mike J.; Walker, Hannah F.; Burns, Julie E.; Collins, Anne T.; Maitland, Norman J.

Authors

Stefanie Hager

Davide Pellacani

Mike J. Stower

Hannah F. Walker

Julie E. Burns

Anne T. Collins

Norman J. Maitland



Abstract

To investigate hierarchy in human prostate epithelial cells, we generated recombinant lentiviruses, infected primary cultures and cell lines, and followed their fate in vitro. The lentiviruses combined constitutive promoters including CMV and β-actin, or late-stage differentiation promoters including PSCA (prostate stem cell antigen) and PSAPb (prostate specific antigen/probasin) driving expression of monomeric, dimeric and tetrameric fluorescent proteins. Significantly, rare CD133+ cells from primary prostate epithelial cultures were successfully infected and activation of late-stage promoters was observed in basal epithelial cultures following induction of differentiation. Lentiviruses also infected CD133+ cells within the P4E6 cell line. However, promoter silencing was observed in several cell lines (P4E6, BPH-1, PC3). We examined the promoter methylation status of the lentiviral insertions in heterogeneously fluorescent cultures from PC3 clones and found that DNA methylation was not the primary mechanism of silencing of the CMV promoter. We also describe limitations to the lentivirus system including technical challenges due to low titers and low infection efficiency in primary cultures. However, we have identified a functional late-stage promoter that indicates differentiation from a basal to a luminal phenotype and demonstrate that this strategy for lineage tracking of prostate epithelial cells is valid with further optimisation.

Journal Article Type Article
Publication Date Nov 15, 2010
Journal Experimental Cell Research
Print ISSN 0014-4827
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 316
Issue 19
Pages 3161-3171
APA6 Citation Frame, F. M., Hager, S., Pellacani, D., Stower, M. J., Walker, H. F., Burns, J. E., …Maitland, N. J. (2010). Development and limitations of lentivirus vectors as tools for tracking differentiation in prostate epithelial cells. Experimental Cell Research, 316(19), 3161-3171. https://doi.org/10.1016/j.yexcr.2010.08.004
DOI https://doi.org/10.1016/j.yexcr.2010.08.004
Keywords Prostate; Epithelial cells; Lentivirus; Fluorescent proteins; Differentiation
Publisher URL https://www.sciencedirect.com/science/article/pii/S0014482710003988?via%3Dihub
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