A feasibility study of a leaky waveguide aptasensor for thrombin

Alamrani, Nasser A.; Greenway, Gillian M.; Pamme, Nicole; Goddard, Nicholas J.; Gupta, Ruchi

Abstract

This proof-of-principle study demonstrates the feasibility of a leaky waveguide (LW) aptasensor, where aptamers were immobilised in a mesoporous chitosan waveguiding film for the detection of thrombin. This work has demonstrated that aptamers immobilised in hydrogels retain their affinity and selectivity towards their target and thus can be used as bioreceptors. The use of antibodies as bioreceptors for sensing thrombin is not viable because it is a serine protease, which will cleave the antibodies. Currently used assays based on clotting time and chromogenic/fluorogenic substrates have limited potential for thrombin measurement in whole blood. Using the initial binding rate over the first 5 min, the limit of detection of our LW aptasensor for thrombin was ∼22 nM. The sensor was tested with spiked serum samples, giving a reading of 46.1 ± 4.6 nM for a sample containing 50 nM thrombin. Our proposed sensor combines the robustness and low cost of aptamers as molecular recognition elements with the simple fabrication process of the chitosan-based leaky waveguide, making LW aptasensors highly attractive for applications in point-of-care diagnostics and healthcare monitoring.

Citation

Alamrani, N. A., Greenway, G. M., Pamme, N., Goddard, N. J., & Gupta, R. (2019). A feasibility study of a leaky waveguide aptasensor for thrombin. Analyst, 144(20), 6048-6054. https://doi.org/10.1039/c9an01421g

• Journal Article Type: Article
• Acceptance Date: Sep 6, 2019
• Online Publication Date: Sep 6, 2019
• Publication Date: Oct 21, 2019
• Deposit Date: Sep 22, 2019
• Publicly Available Date: Sep 7, 2020
• Journal: Analyst
• Print ISSN: 0003-2654
• Electronic ISSN: 1364-5528
• Publisher: Royal Society of Chemistry
• Peer Reviewed: Peer Reviewed
• Volume: 144
• Issue: 20
• Pages: 6048-6054
• DOI: https://doi.org/10.1039/c9an01421g
• Keywords: Analytical Chemistry; Spectroscopy; Electrochemistry; Biochemistry; Environmental Chemistry
• Public URL: https://hull-repository.worktribe.com/output/2720696
• Publisher URL: https://pubs.rsc.org/en/content/articlelanding/2019/an/c9an01421g/unauth#!divAbstract
• Additional Information: : This document is Similarity Check deposited; : Gillian M. Greenway (ORCID); : Nicole Pamme (ORCID); : Nicole Pamme (ResearcherID); : Ruchi Gupta (ORCID); : Ruchi Gupta (ResearcherID); : Single-blind; : Received 27 July 2019; Accepted 6 September 2019; Accepted Manuscript published 6 September 2019; Advance Article published 13 September 2019
• Contract Date: Sep 26, 2019