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Transcriptomic Adjustments of Staphylococcus aureus COL (MRSA) Forming Biofilms Under Acidic and Alkaline Conditions

Efthimiou, Georgios; Tsiamis, Georgios ; Typas, Milton; Pappas, Katherine

Authors

Georgios Tsiamis

Milton Typas

Katherine Pappas



Abstract

© Copyright © 2019 Efthimiou, Tsiamis, Typas and Pappas. Methicillin-resistant Staphylococcus aureus (MRSA) strains are important human pathogens and a significant health hazard for hospitals and the food industry. They are resistant to β-lactam antibiotics including methicillin and extremely difficult to treat. In this study, we show that the Staphylococcus aureus COL (MRSA) strain, with a known complete genome, can easily survive and grow under acidic and alkaline conditions (pH5 and pH9, respectively), both planktonically and as a biofilm. A microarray-based analysis of both planktonic and biofilm cells was performed under acidic and alkaline conditions showing that several genes are up- or down-regulated under different environmental conditions and growth modes. These genes were coding for transcription regulators, ion transporters, cell wall biosynthetic enzymes, autolytic enzymes, adhesion proteins and antibiotic resistance factors, most of which are associated with biofilm formation. These results will facilitate a better understanding of the physiological adjustments occurring in biofilm-associated S. aureus COL cells growing in acidic or alkaline environments, which will enable the development of new efficient treatment or disinfection strategies.

Citation

Efthimiou, G., Tsiamis, G., Typas, M., & Pappas, K. (2019). Transcriptomic Adjustments of Staphylococcus aureus COL (MRSA) Forming Biofilms Under Acidic and Alkaline Conditions. Frontiers in Microbiology, 10, https://doi.org/10.3389/fmicb.2019.02393

Journal Article Type Article
Acceptance Date Oct 2, 2019
Publication Date Oct 18, 2019
Deposit Date Oct 7, 2019
Publicly Available Date Oct 8, 2019
Journal Frontiers in Microbiology
Print ISSN 1664-302X
Electronic ISSN 1664-302X
Publisher Frontiers Media
Peer Reviewed Peer Reviewed
Volume 10
Article Number 02393
DOI https://doi.org/10.3389/fmicb.2019.02393
Keywords MRSA; Biofilm (BF); Alkaline; Acidic; Microarray; Transcription 28
Public URL https://hull-repository.worktribe.com/output/2876246
Publisher URL https://www.frontiersin.org/articles/10.3389/fmicb.2019.02393/full

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Publisher Licence URL
https://creativecommons.org/licenses/by/4.0/

Copyright Statement
© 2019 Efthimiou, Tsiamis, Typas and Pappas. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.


Published article (563 Kb)
PDF

Publisher Licence URL
https://creativecommons.org/licenses/by/4.0/

Copyright Statement
© 2019 Efthimiou, Tsiamis, Typas and Pappas. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.







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