Skip to main content

Research Repository

Advanced Search

Isolated tumour microparticles induce endothelial microparticle release in vitro

Faulkner, Lucy G.; Alqarni, Saeed; Maraveyas, Anthony; Madden, Leigh A.

Authors

Lucy G. Faulkner

Saeed Alqarni

Anthony Maraveyas



Abstract

© 2019 Wolters Kluwer Health, Inc. All rights reserved. Cancer induces a hypercoagulable state, resulting in an increased risk of venous thromboembolism. One of the mechanisms driving this is tissue factor (TF) production by the tumour, released in small lipid bound microparticles. We have previously demonstrated that tumour cell line media-induced procoagulant changes in HUVEC. The aim of this study was to investigate the effect of tumour microparticles and recombinant human TF (rhTF) on the endothelium. Procoagulant microparticles from the PANC-1 cell line were harvested by ultrafiltration. HUVEC were then incubated with these procoagulant microparticles or rhTF. Flow cytometry was used to investigate the effect of endothelial cell surface protein expression and microparticle release. Microparticles but not soluble TF was responsible for the procoagulant activity of cell-free tumour media. We also demonstrated an increase in endothelial microparticle release with exposure to tumour microparticles, with a positive linear relationship observed (R2 = 0.6630 P ≤ 0.0001). rhTF did not induce any of the changes observed with microparticles. Here we demonstrate that procoagulant activity of tumour cell line media is dependent on microparticles, and that exposure of endothelial cells to these microparticles results in an increase in microparticle release from HUVEC. This suggests a mechanism of transfer of procoagulant potential from the cancer to the remote endothelium.

Citation

Faulkner, L. G., Alqarni, S., Maraveyas, A., & Madden, L. A. (2020). Isolated tumour microparticles induce endothelial microparticle release in vitro. Blood Coagulation and Fibrinolysis, 31(1), 35-42. https://doi.org/10.1097/MBC.0000000000000876

Journal Article Type Article
Acceptance Date Nov 5, 2019
Online Publication Date Nov 26, 2019
Publication Date Jan 1, 2020
Deposit Date Nov 25, 2019
Publicly Available Date Nov 27, 2020
Journal Blood Coagulation and Fibrinolysis
Print ISSN 0957-5235
Electronic ISSN 1473-5733
Publisher Lippincott, Williams & Wilkins
Peer Reviewed Peer Reviewed
Volume 31
Issue 1
Pages 35-42
DOI https://doi.org/10.1097/MBC.0000000000000876
Keywords Microparticles; Microvesicles; Venous thromboembolism; Endothelium; Cancer.
Public URL https://hull-repository.worktribe.com/output/3155923

Files





You might also like



Downloadable Citations