Skip to main content

Microcrystalline testing used in combination with Raman micro-spectroscopy for absolute identification of novel psychoactive substances: Microcrystalline testing used in combination with Raman micro-spectroscopy

Elie, Leonie; Elie, Mathieu; Cave, Gareth; Vetter, Marleen; Croxton, Ruth; Baron, Mark

Authors

Leonie Elie

Mathieu Elie

Gareth Cave

Marleen Vetter

Mark Baron



Abstract

Two new psychoactive substances, namely 4-methylmethcathinone (mephedrone) and 5,6-methylenedioxy-2-aminoindane (MDAI) were analysed with a novel combination of microcrystalline tests followed by Raman micro-spectroscopy to facilitate their absolute identification. The discrimination power of the proposed combination was successfully demonstrated through the analysis of the positional isomers 2- and 3-methylmethcathinone. The addition of mercury dichloride as a microcrystalline test reagent produced specific microcrystals of each tested analyte. The robustness of the method was evaluated in the presence of common cutting agents (caffeine and benzocaine) as well as on street samples. The crystal lattice structures of mephedrone, 2methylmethcathinone and MDAI mercury dichloride microcrystals were determined by single crystal X-ray diffraction. This confirmed the presence of both drug and reagent together in the lattice and accounts for the distinct habit of the observed microcrystals. Raman spectra of the formed microcrystals differed from those obtained from their standard salt form by loss and/or gain of some vibrational modes. Particularly important was the appearance of the mercury chloride link to each tested drug molecule which showed as strong bands at low wavenumbers. Its presence was corroborated by its detection in the crystal lattice. It was therefore concluded that microcrystalline testing followed by Raman micro-spectroscopy satisfies the technique combination requirement for psychoactive substances recommended by the Scientific Working Group for the Analysis of Seized Drugs (SWGDRUG) and provides a rapid and cheap analysis route. The proposed technique combination also aids the development of new microcrystalline tests as it allows for confirmation of the uniqueness of the developed microcrystals almost in-situ rather than growing single crystals for often long periods of time needed for single crystal X-ray diffraction analysis.

Journal Article Type Article
Publication Date 2016-11
Journal Journal of Raman Spectroscopy
Print ISSN 0377-0486
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 47
Issue 11
Pages 1343-1350
APA6 Citation Elie, L., Elie, M., Cave, G., Vetter, M., Croxton, R., & Baron, M. (2016). Microcrystalline testing used in combination with Raman micro-spectroscopy for absolute identification of novel psychoactive substances: Microcrystalline testing used in combination with Raman micro-spectroscopy. Journal of Raman Spectroscopy, 47(11), 1343-1350. https://doi.org/10.1002/jrs.4957
DOI https://doi.org/10.1002/jrs.4957
Keywords Raman micro-spectroscopy; Forensic Science; microcrystalline test; new psychoactive substances
Publisher URL https://onlinelibrary.wiley.com/doi/abs/10.1002/jrs.4957
Related Public URLs http://eprints.lincoln.ac.uk/id/eprint/27905/

Files

Article (2.5 Mb)
PDF

Copyright Statement
©2020 The authors. All rights reserved. No part of this publication may be reproduced without the written permission of the copyright holder





You might also like



Downloadable Citations

;