Enhanced exercise and regenerative capacity in a mouse model that violates size constraints of oxidative muscle fibres

Omairi, Saleh; Matsakas, Antonios; Degens, Hans; Kretz, Oliver; Hansson, Kenth Arne; Solbrå, Andreas Våvang; Bruusgaard, Jo C; Joch, Barbara; Sartori, Roberta; Giallourou, Natasa; Mitchell, Robert; Collins-Hooper, Henry; Foster, Keith; Pasternack, Arja; Ritvos, Olli; Sandri, Marco; Narkar, Vihang; Swann, Jonathan R; Huber, Tobias B; Patel, Ketan

Abstract

A central tenet of skeletal muscle biology is the existence of an inverse relationship between the oxidative fibre capacity and its size. However, robustness of this relationship is unknown. We show that superimposition of Estrogen-related receptor gamma (Errγ) on the myostatin (Mtn) mouse null background (Mtn-/-/ErrγTg/+) results in hypertrophic muscle with a high oxidative capacity thus violating the inverse relationship between fibre size and oxidative capacity. We also examined the canonical view that oxidative muscle phenotype positively correlate with Satellite cell number, the resident stem cells of skeletal muscle. Surprisingly, hypertrophic fibres from Mtn-/-/ErrγTg/+ mouse showed satellite cell deficit which unexpectedly did not affect muscle regeneration. These observations 1) challenge the concept of a constraint between fibre size and oxidative capacity and 2) indicate the important role of the microcirculation in the regenerative capacity of a muscle even when satellite cell numbers are reduced.

Citation

Omairi, S., Matsakas, A., Degens, H., Kretz, O., Hansson, K. A., Solbrå, A. V., Bruusgaard, J. C., Joch, B., Sartori, R., Giallourou, N., Mitchell, R., Collins-Hooper, H., Foster, K., Pasternack, A., Ritvos, O., Sandri, M., Narkar, V., Swann, J. R., Huber, T. B., & Patel, K. (2016). Enhanced exercise and regenerative capacity in a mouse model that violates size constraints of oxidative muscle fibres. eLife, 5(AUGUST), Article 16940. https://doi.org/10.7554/elife.16940

• Journal Article Type: Article
• Acceptance Date: Jul 19, 2016
• Online Publication Date: Aug 5, 2016
• Publication Date: Aug 5, 2016
• Deposit Date: Jan 28, 2020
• Publicly Available Date: Mar 20, 2020
• Journal: eLife
• Electronic ISSN: 2050-084X
• Publisher: eLife Sciences Publications
• Peer Reviewed: Peer Reviewed
• Volume: 5
• Issue: AUGUST
• Article Number: 16940
• DOI: https://doi.org/10.7554/elife.16940
• Public URL: https://hull-repository.worktribe.com/output/3395161
• Publisher URL: https://elifesciences.org/articles/16940