Protein arginine methyl transferases-3 and -5 increase cell surface expression of cardiac sodium channel

Beltran-Alvarez, Pedro; Espejo, Alexsandra; Schmauder, Ralf; Beltran, Carlos; Mrowka, Ralf; Linke, Thomas; Batlle, Montserrat; Pérez-Villa, Félix; Pérez, Guillermo J.; Scornik, Fabiana S.; Benndorf, Klaus; Pagans, Sara; Zimmer, Thomas; Brugada, Ramon

doi:10.1016/j.febslet.2013.07.043

Protein arginine methyl transferases-3 and -5 increase cell surface expression of cardiac sodium channel

Beltran-Alvarez, Pedro; Espejo, Alexsandra; Schmauder, Ralf; Beltran, Carlos; Mrowka, Ralf; Linke, Thomas; Batlle, Montserrat; Pérez-Villa, Félix; Pérez, Guillermo J.; Scornik, Fabiana S.; Benndorf, Klaus; Pagans, Sara; Zimmer, Thomas; Brugada, Ramon

Authors

Dr Pedro Beltran-Alvarez P.Beltran-Alvarez@hull.ac.uk
Senior Lecturer in Health and Climate Change and Programme co-Director of the MSc Health and Climate Change

Alexsandra Espejo

Ralf Schmauder

Carlos Beltran

Ralf Mrowka

Thomas Linke

Montserrat Batlle

Félix Pérez-Villa

Guillermo J. Pérez

Fabiana S. Scornik

Klaus Benndorf

Sara Pagans

Thomas Zimmer

Ramon Brugada

Abstract

The α-subunit of the cardiac voltage-gated sodium channel (Na V1.5) plays a central role in cardiomyocyte excitability. We have recently reported that NaV1.5 is post-translationally modified by arginine methylation. Here, we aimed to identify the enzymes that methylate NaV1.5, and to describe the role of arginine methylation on Na V1.5 function. Our results show that protein arginine methyl transferase (PRMT)-3 and -5 methylate NaV1.5 in vitro, interact with NaV1.5 in human embryonic kidney (HEK) cells, and increase Na V1.5 current density by enhancing NaV1.5 cell surface expression. Our observations are the first evidence of regulation of a voltage-gated ion channel, including calcium, potassium, sodium and TRP channels, by arginine methylation. © 2013 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

Citation

Beltran-Alvarez, P., Espejo, A., Schmauder, R., Beltran, C., Mrowka, R., Linke, T., Batlle, M., Pérez-Villa, F., Pérez, G. J., Scornik, F. S., Benndorf, K., Pagans, S., Zimmer, T., & Brugada, R. (2013). Protein arginine methyl transferases-3 and -5 increase cell surface expression of cardiac sodium channel. FEBS Letters, 587(19), 3159-3165. https://doi.org/10.1016/j.febslet.2013.07.043

Journal Article Type	Article
Acceptance Date	Jul 16, 2013
Online Publication Date	Jul 31, 2013
Publication Date	Oct 1, 2013
Deposit Date	Apr 1, 2022
Journal	FEBS Letters
Print ISSN	0014-5793
Publisher	Wiley
Peer Reviewed	Peer Reviewed
Volume	587
Issue	19
Pages	3159-3165
DOI	https://doi.org/10.1016/j.febslet.2013.07.043
Public URL	https://hull-repository.worktribe.com/output/3570068