A systematic screen for proteing-lipid interactions in Saccharomyces cerevisiae

Gallego, Oriol; Betts, Matthew J.; Gvozdenovic-Jeremic, Jelena; Maeda, Kenji; Matetzki, Christian; Aguilar-Gurrieri, Carmen; Beltran-Alvarez, Pedro; Bonn, Stefan; Fernández-Tornero, Carlos; Jensen, Lars Juhl; Kuhn, Michael; Trott, Jamie; Rybin, Vladimir; Müller, Christoph W.; Bork, Peer; Kaksonen, Marko; Russell, Robert B.; Gavin, Anne Claude

Abstract

Proteing-metabolite networks are central to biological systems, but are incompletely understood. Here, we report a screen to catalog proteing-lipid interactions in yeast. We used arrays of 56 metabolites to measure lipid-binding fingerprints of 172 proteins, including 91 with predicted lipid-binding domains. We identified 530 proteing-lipid associations, the majority of which are novel. To show the data set's biological value, we studied further several novel interactions with sphingolipids, a class of conserved bioactive lipids with an elusive mode of action. Integration of live-cell imaging suggests new cellular targets for these molecules, including several with pleckstrin homology (PH) domains. Validated interactions with Slm1, a regulator of actin polarization, show that PH domains can have unexpected lipid-binding specificities and can act as coincidence sensors for both phosphatidylinositol phosphates and phosphorylated sphingolipids. © 2010 EMBO and Macmillan Publishers Limited All rights reserved.

Citation

Gallego, O., Betts, M. J., Gvozdenovic-Jeremic, J., Maeda, K., Matetzki, C., Aguilar-Gurrieri, C., Beltran-Alvarez, P., Bonn, S., Fernández-Tornero, C., Jensen, L. J., Kuhn, M., Trott, J., Rybin, V., Müller, C. W., Bork, P., Kaksonen, M., Russell, R. B., & Gavin, A. C. (2010). A systematic screen for proteing-lipid interactions in Saccharomyces cerevisiae. Molecular Systems Biology, 6(1), Article 430. https://doi.org/10.1038/msb.2010.87

• Journal Article Type: Article
• Acceptance Date: Oct 4, 2010
• Online Publication Date: Nov 30, 2010
• Publication Date: 2010-01
• Deposit Date: Apr 1, 2022
• Publicly Available Date: Apr 21, 2022
• Journal: Molecular Systems Biology
• Print ISSN: 1744-4292
• Electronic ISSN: 1744-4292
• Publisher: EMBO Press
• Peer Reviewed: Peer Reviewed
• Volume: 6
• Issue: 1
• Article Number: 430
• DOI: https://doi.org/10.1038/msb.2010.87
• Public URL: https://hull-repository.worktribe.com/output/3570117