Effects of antagonist of retinoid X receptor (UVI3003) on morphology and gene profile of Xenopus tropicalis embryos

Zhu, Jingmin; Shi, Huahong; Zhu, Pan; Hu, Lingling; Wu, Lijiao; Yang, Yi; Rotchell, Jeanette M.

Abstract

We exposed Xenopus tropicalis embryos to a selective antagonist of retinoid X receptor (UVI3003). UVI3003 induced multiple malformations at the concentrations of 200-1000. μg/L after 48. h exposure. The most prominent malformations affected brains, eyes, cement gland and fins. UVI3003 also induced variable and divergent malformations at 250-1500. μg/L after 0-24 and 24-48. h exposure. Microarray analysis showed that seven genes (rps15, serp2, fmr1, cyp2e1, lrrc9, ugtla6 and LOC100490188) were differentially regulated in all three treatment groups after 0-24. h exposure. The most significantly affected pathway was galactose metabolism. In 24-48. h exposure groups, 18 genes were differentially regulated, mainly comprising components of the PPAR signaling pathway. These results suggested that UVI3003 is teratogenic in amphibian embryos. Differential gene expression suggests that galactose metabolism and PPAR signaling pathways may provide underlying mechanistic detail accounting for the observed malformations. © 2014 Elsevier B.V.

Citation

Zhu, J., Shi, H., Zhu, P., Hu, L., Wu, L., Yang, Y., & Rotchell, J. M. (2014). Effects of antagonist of retinoid X receptor (UVI3003) on morphology and gene profile of Xenopus tropicalis embryos. Environmental Toxicology and Pharmacology, 38(1), 153-162. https://doi.org/10.1016/j.etap.2014.05.010

• Journal Article Type: Article
• Acceptance Date: May 19, 2014
• Online Publication Date: May 27, 2014
• Publication Date: 2014-07
• Deposit Date: Apr 2, 2022
• Journal: Environmental Toxicology and Pharmacology
• Print ISSN: 1382-6689
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 38
• Issue: 1
• Pages: 153-162
• DOI: https://doi.org/10.1016/j.etap.2014.05.010
• Keywords: Amphibian; Retinoid X receptor; Antagonist; UVI3003; Teratogenicity
• Public URL: https://hull-repository.worktribe.com/output/3621128