pH dependent binding in de novo hetero bimetallic coiled coils

Peacock, Anna F.A.; Jeuken, Lars J.C.; Ciani, Barbara; Anbu, Sellamuthu; Adams, Samuel J.; Smith, Caitlin F.; Teare, Paul

Abstract

© The Royal Society of Chemistry. Herein the first example of a bimetallic coiled coil featuring a lanthanide binding site is reported, opening opportunities to exploit the attractive NMR and photophysical properties of the lanthanides in multi metallo protein design. In our efforts to fully characterise the system we identified for the first time that lanthanide binding to such sites is pH dependent, with optimal binding at neutral pH, and that the double AsnAsp site is more versatile in this regard than the single Asp site. Our second site featured the structural HgCys3 site, the chemistry of which was essentially unaltered by the presence of the lanthanide site. In fact, both metal binding sites within the hetero bimetallic coiled coil displayed the same properties as their mononuclear single binding site controls, and operated independently of each other. Finally, pH can be used as an external trigger to control the binding of Hg(ii) and Tb(iii) to the two distinct sites within this coiled coil, and offers the opportunity to “activate” metal binding sites within complex multi metallo and multi-functional designs.

Citation

Peacock, A. F., Jeuken, L. J., Ciani, B., Anbu, S., Adams, S. J., Smith, C. F., & Teare, P. (2018). pH dependent binding in de novo hetero bimetallic coiled coils. Dalton Transactions : an international journal of inorganic chemistry, 47(31), 10784-10790. https://doi.org/10.1039/c8dt01568f

• Journal Article Type: Article
• Acceptance Date: Jul 5, 2018
• Online Publication Date: Jul 5, 2018
• Publication Date: Aug 21, 2018
• Deposit Date: Apr 9, 2021
• Publicly Available Date: May 26, 2021
• Journal: Dalton Transactions
• Print ISSN: 1477-9226
• Electronic ISSN: 1477-9234
• Publisher: Royal Society of Chemistry
• Peer Reviewed: Peer Reviewed
• Volume: 47
• Issue: 31
• Pages: 10784-10790
• DOI: https://doi.org/10.1039/c8dt01568f
• Public URL: https://hull-repository.worktribe.com/output/3739423
• Additional Information: : This document is Similarity Check deposited; : Supplementary Information; : Sellamuthu Anbu (ORCID); : Lars J. C. Jeuken (ORCID); : Anna F. A. Peacock (ORCID); : Single-blind; : Received 20 April 2018; Accepted 5 July 2018; Accepted Manuscript published 5 July 2018; Advance Article published 19 July 2018; Version of Record published 7 August 2018