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Short communication: Biofilm production characterization of mecA and mecC methicillin-resistant Staphylococcus aureus isolated from bovine milk in Great Britain

Sitja, M.; Prenafeta, A.; Sitjà, M.; Holmes, M. A.; Paterson, G. K.

Authors

M. Sitja

A. Prenafeta

M. Sitjà

M. A. Holmes

G. K. Paterson



Abstract

Staphylococcus aureus is an important cause of contagious intramammary infection in dairy cattle and the ability to produce biofilm is considered to be an important virulence property in the pathogenesis of mastitis. The aim of this study was to characterize the biofilm formation capacity of methicillin-resistant S. aureus (MRSA), encoding mecA or mecC, isolated from bulk tank milk in Great Britain. For this purpose, 20 MRSA isolates were grown on microtiter plates to determine the biofilm production. Moreover, the presence of the intercellular adhesion genes icaA and icaD were analyzed by PCR. All the tested MRSA isolates were PCR positive for the ica genes and 10 of them (50%) produced biofilm in the microtiter plate assay. This is also the first demonstration of biofilm production by mecC MRSA.

Citation

Prenafeta, A., Sitjà, M., Holmes, M. A., & Paterson, G. K. (2014). Short communication: Biofilm production characterization of mecA and mecC methicillin-resistant Staphylococcus aureus isolated from bovine milk in Great Britain. Journal of dairy science, 97(8), 4838-4841 . https://doi.org/10.3168/jds.2014-7986

Acceptance Date Apr 17, 2014
Online Publication Date Jun 2, 2014
Publication Date 2014-08
Deposit Date Jun 23, 2015
Publicly Available Date Jun 23, 2015
Journal Journal of dairy science
Print ISSN 0022-0302
Electronic ISSN 1525-3198
Publisher American Dairy Science Association
Peer Reviewed Peer Reviewed
Volume 97
Issue 8
Pages 4838-4841
DOI https://doi.org/10.3168/jds.2014-7986
Keywords Staphylococcus aureus; Methicillin-resistant; Staphylococcus aureus; Biofilm
Public URL https://hull-repository.worktribe.com/output/375621
Publisher URL http://www.sciencedirect.com/science/article/pii/S0022030214003877
Additional Information Author's accepted manuscript of article published in: Journal of dairy science, 2014, v.97, issue 8

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Publisher Licence URL
http://creativecommons.org/licenses/by-nc-nd/4.0/

Copyright Statement
© 2014, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/



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