Short communication: Biofilm production characterization of mecA and mecC methicillin-resistant Staphylococcus aureus isolated from bovine milk in Great Britain
Sitja, M.; Prenafeta, A.; Sitjà, M.; Holmes, M. A.; Paterson, G. K.
M. A. Holmes
G. K. Paterson
Staphylococcus aureus is an important cause of contagious intramammary infection in dairy cattle and the ability to produce biofilm is considered to be an important virulence property in the pathogenesis of mastitis. The aim of this study was to characterize the biofilm formation capacity of methicillin-resistant S. aureus (MRSA), encoding mecA or mecC, isolated from bulk tank milk in Great Britain. For this purpose, 20 MRSA isolates were grown on microtiter plates to determine the biofilm production. Moreover, the presence of the intercellular adhesion genes icaA and icaD were analyzed by PCR. All the tested MRSA isolates were PCR positive for the ica genes and 10 of them (50%) produced biofilm in the microtiter plate assay. This is also the first demonstration of biofilm production by mecC MRSA.
|Journal||Journal of dairy science|
|Publisher||American Dairy Science Association|
|Peer Reviewed||Peer Reviewed|
|Institution Citation||Prenafeta, A., Sitjà, M., Holmes, M. A., & Paterson, G. K. (2014). Short communication: Biofilm production characterization of mecA and mecC methicillin-resistant Staphylococcus aureus isolated from bovine milk in Great Britain. Journal of dairy science, 97(8), 4838-4841. https://doi.org/10.3168/jds.2014-7986|
|Keywords||Staphylococcus aureus; Methicillin-resistant; Staphylococcus aureus; Biofilm|
|Additional Information||Author's accepted manuscript of article published in: Journal of dairy science, 2014, v.97, issue 8|
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