Simultaneous bioassays in a microfluidic channel on plugs of different magnetic particles

Bronzeau, Sandrine; Pamme, Nicole

Abstract

Magnetic particles coated with specific biomolecules are often used as solid supports for bioassays but conventional test tube based techniques are time consuming and labour intensive. An alternative is to work on magnetic particle plugs immobilised inside microfluidic channels. Most research so far has focussed on immobilising one type of particle to perform one type of assay. Here we demonstrate how several assays can be performed simultaneously by flushing a sample solution over several plugs of magnetic particles with different surface coatings. Within a microchannel, three plugs of magnetic particles were immobilised with external magnets. The particles featured surface coatings of glycine, streptavidin and protein A, respectively. Reagents were then flushed through the three plugs. Molecular binding occurred between matching antigens and antibodies in continuous flow and was detected by fluorescence. This first demonstration opens the door to a quicker and easier technique for simultaneous bioassays using magnetic particles. © 2007 Elsevier B.V. All rights reserved.

Citation

Bronzeau, S., & Pamme, N. (2008). Simultaneous bioassays in a microfluidic channel on plugs of different magnetic particles. Analytica Chimica Acta, 609(1), 105-112. https://doi.org/10.1016/j.aca.2007.11.035

• Journal Article Type: Article
• Acceptance Date: Nov 19, 2007
• Online Publication Date: Nov 28, 2007
• Publication Date: Feb 18, 2008
• Journal: ANALYTICA CHIMICA ACTA
• Print ISSN: 0003-2670
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 609
• Issue: 1
• Pages: 105-112
• DOI: https://doi.org/10.1016/j.aca.2007.11.035
• Keywords: Magnetic particles; Microfluidics; Assay; Simultaneous analysis
• Public URL: https://hull-repository.worktribe.com/output/395991
• Publisher URL: https://www.sciencedirect.com/science/article/pii/S0003267007019186?via%3Dihub