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Simultaneous bioassays in a microfluidic channel on plugs of different magnetic particles

Bronzeau, Sandrine; Pamme, Nicole

Authors

Sandrine Bronzeau



Abstract

Magnetic particles coated with specific biomolecules are often used as solid supports for bioassays but conventional test tube based techniques are time consuming and labour intensive. An alternative is to work on magnetic particle plugs immobilised inside microfluidic channels. Most research so far has focussed on immobilising one type of particle to perform one type of assay. Here we demonstrate how several assays can be performed simultaneously by flushing a sample solution over several plugs of magnetic particles with different surface coatings. Within a microchannel, three plugs of magnetic particles were immobilised with external magnets. The particles featured surface coatings of glycine, streptavidin and protein A, respectively. Reagents were then flushed through the three plugs. Molecular binding occurred between matching antigens and antibodies in continuous flow and was detected by fluorescence. This first demonstration opens the door to a quicker and easier technique for simultaneous bioassays using magnetic particles. © 2007 Elsevier B.V. All rights reserved.

Citation

Bronzeau, S., & Pamme, N. (2008). Simultaneous bioassays in a microfluidic channel on plugs of different magnetic particles. Analytica Chimica Acta, 609(1), 105-112. https://doi.org/10.1016/j.aca.2007.11.035

Journal Article Type Article
Acceptance Date Nov 19, 2007
Online Publication Date Nov 28, 2007
Publication Date Feb 18, 2008
Journal ANALYTICA CHIMICA ACTA
Print ISSN 0003-2670
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 609
Issue 1
Pages 105-112
DOI https://doi.org/10.1016/j.aca.2007.11.035
Keywords Magnetic particles; Microfluidics; Assay; Simultaneous analysis
Public URL https://hull-repository.worktribe.com/output/395991
Publisher URL https://www.sciencedirect.com/science/article/pii/S0003267007019186?via%3Dihub